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Hypoxia/Hypercapnia-Induced Adaptation Maintains Functional Capacity of Cord Blood Stem and Progenitor Cells at 4 degrees C
dc.creator | Vlaški, Marija | |
dc.creator | Negroni, Luc | |
dc.creator | Kovačević-Filipović, Milica | |
dc.creator | Guibert, Christelle | |
dc.creator | de la Grange, Philippe Brunet | |
dc.creator | Rossignol, Rodrigue | |
dc.creator | Chevaleyre, Jean | |
dc.creator | Duchez, Pascale | |
dc.creator | Lafarge, Xavier | |
dc.creator | Praloran, Vincent | |
dc.creator | Schmitter, Jean-Marie | |
dc.creator | Ivanović, Zoran | |
dc.date.accessioned | 2020-06-03T13:49:39Z | |
dc.date.available | 2020-06-03T13:49:39Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 0021-9541 | |
dc.identifier.uri | https://vet-erinar.vet.bg.ac.rs/handle/123456789/1118 | |
dc.description.abstract | We analyzed the effect of exposure to hypoxic/hypercapnic (HH) gas mixture (5% O2/9% CO2) on the maintenance of functional cord blood CD34+ hematopoietic stem and progenitor cells in severe hypothermia (4 degrees C) employing the physiological and proteomic approaches. Ten-day exposure to HH maintained the Day 0 (D-0) level of hematopoietic stem cells as detected in vivo on the basis of hematopoietic repopulation of immunodeficient miceshort-term scid repopulating cells (SRC). Conversely, in the atmospheric air (20% O2/0.05% CO2), usual condition used for cell storage at 4 degrees C, stem cell activity was significantly decreased. Also, HH doubled the survival of CD34+ cells and committed progenitors (CFCs) with respect to the atmospheric air (60% vs. 30%, respectively). Improved cell maintenance in HH was associated with higher proportion of aldehyde dehydrogenase (ALDH) positive cells. Cell-protective effects are associated with an improved maintenance of the plasma and mitochondrial membrane potential and with a conversion to the glycolytic energetic state. We also showed that HH decreased apoptosis, despite a sustained ROS production and a drop of ATP amount per viable cell. The proteomic study revealed that the global protein content was better preserved in HH. This analysis identified: (i) proteins sensitive or insensitive to hypothermia irrespective of the gas phase, and (ii) proteins related to the HH cell-protective effect. Among them are some protein families known to be implicated in the prolonged survival of hibernating animals in hypothermia. These findings suggest a way to optimize short-term cell conservation without freezing. J. Cell. Physiol. 229: 2153-2165, 2014. | en |
dc.publisher | Wiley, Hoboken | |
dc.relation | Fondation Jerome Lejeune/Novussanguis Consortium | |
dc.relation | International Research Group on Hematopoietic Cell Transplantation (IRGHET) | |
dc.relation | French Blood Institute [APR 2013] | |
dc.relation | Regional R&D Aquitaine-Limousin Budget | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175061/RS// | |
dc.rights | restrictedAccess | |
dc.source | Journal of Cellular Physiology | |
dc.title | Hypoxia/Hypercapnia-Induced Adaptation Maintains Functional Capacity of Cord Blood Stem and Progenitor Cells at 4 degrees C | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Ивановић, Зоран; де ла Гранге, Пхилиппе Брунет; Россигнол, Родригуе; Сцхмиттер, Јеан-Марие; Негрони, Луц; Влашки, Марија; Гуиберт, Цхристелле; Пралоран, Винцент; Цхевалеyре, Јеан; Ковачевић-Филиповић, Милица; Дуцхез, Пасцале; Лафарге, Xавиер; | |
dc.citation.volume | 229 | |
dc.citation.issue | 12 | |
dc.citation.spage | 2153 | |
dc.citation.epage | 2165 | |
dc.citation.other | 229(12): 2153-2165 | |
dc.citation.rank | M21 | |
dc.identifier.wos | 000341647400031 | |
dc.identifier.doi | 10.1002/jcp.24678 | |
dc.identifier.pmid | 24912010 | |
dc.identifier.scopus | 2-s2.0-84904961282 | |
dc.type.version | publishedVersion |