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dc.creatorVlaški, Marija
dc.creatorNegroni, Luc
dc.creatorKovačević-Filipović, Milica
dc.creatorGuibert, Christelle
dc.creatorde la Grange, Philippe Brunet
dc.creatorRossignol, Rodrigue
dc.creatorChevaleyre, Jean
dc.creatorDuchez, Pascale
dc.creatorLafarge, Xavier
dc.creatorPraloran, Vincent
dc.creatorSchmitter, Jean-Marie
dc.creatorIvanović, Zoran
dc.date.accessioned2020-06-03T13:49:39Z
dc.date.available2020-06-03T13:49:39Z
dc.date.issued2014
dc.identifier.issn0021-9541
dc.identifier.urihttps://vet-erinar.vet.bg.ac.rs/handle/123456789/1118
dc.description.abstractWe analyzed the effect of exposure to hypoxic/hypercapnic (HH) gas mixture (5% O2/9% CO2) on the maintenance of functional cord blood CD34+ hematopoietic stem and progenitor cells in severe hypothermia (4 degrees C) employing the physiological and proteomic approaches. Ten-day exposure to HH maintained the Day 0 (D-0) level of hematopoietic stem cells as detected in vivo on the basis of hematopoietic repopulation of immunodeficient miceshort-term scid repopulating cells (SRC). Conversely, in the atmospheric air (20% O2/0.05% CO2), usual condition used for cell storage at 4 degrees C, stem cell activity was significantly decreased. Also, HH doubled the survival of CD34+ cells and committed progenitors (CFCs) with respect to the atmospheric air (60% vs. 30%, respectively). Improved cell maintenance in HH was associated with higher proportion of aldehyde dehydrogenase (ALDH) positive cells. Cell-protective effects are associated with an improved maintenance of the plasma and mitochondrial membrane potential and with a conversion to the glycolytic energetic state. We also showed that HH decreased apoptosis, despite a sustained ROS production and a drop of ATP amount per viable cell. The proteomic study revealed that the global protein content was better preserved in HH. This analysis identified: (i) proteins sensitive or insensitive to hypothermia irrespective of the gas phase, and (ii) proteins related to the HH cell-protective effect. Among them are some protein families known to be implicated in the prolonged survival of hibernating animals in hypothermia. These findings suggest a way to optimize short-term cell conservation without freezing. J. Cell. Physiol. 229: 2153-2165, 2014.en
dc.publisherWiley, Hoboken
dc.relationFondation Jerome Lejeune/Novussanguis Consortium
dc.relationInternational Research Group on Hematopoietic Cell Transplantation (IRGHET)
dc.relationFrench Blood Institute [APR 2013]
dc.relationRegional R&D Aquitaine-Limousin Budget
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175061/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Cellular Physiology
dc.titleHypoxia/Hypercapnia-Induced Adaptation Maintains Functional Capacity of Cord Blood Stem and Progenitor Cells at 4 degrees Cen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractИвановић, Зоран; де ла Гранге, Пхилиппе Брунет; Россигнол, Родригуе; Сцхмиттер, Јеан-Марие; Негрони, Луц; Влашки, Марија; Гуиберт, Цхристелле; Пралоран, Винцент; Цхевалеyре, Јеан; Ковачевић-Филиповић, Милица; Дуцхез, Пасцале; Лафарге, Xавиер;
dc.citation.volume229
dc.citation.issue12
dc.citation.spage2153
dc.citation.epage2165
dc.citation.other229(12): 2153-2165
dc.citation.rankM21
dc.identifier.wos000341647400031
dc.identifier.doi10.1002/jcp.24678
dc.identifier.pmid24912010
dc.identifier.scopus2-s2.0-84904961282
dc.type.versionpublishedVersion


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