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Klasična kuga svinja - aktivna imunizacija prasadi subjediničnom (E2) vakcinom u prisustvu različitog nivoa kolostralnog imuniteta

dc.creatorProdanov-Radulović, Jasna
dc.creatorDošen, Radoslav
dc.creatorPolaček, Vladimir
dc.creatorPetrović, Tamaš
dc.creatorStojanov, Igor
dc.creatorRatajac, Radomir
dc.creatorValčić, Miroslav
dc.date.accessioned2020-06-03T13:50:58Z
dc.date.available2020-06-03T13:50:58Z
dc.date.issued2014
dc.identifier.issn0567-8315
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1137
dc.description.abstractThe aim of this study was to investigate the efficacy of the subunit vaccine against virulent CSF infection in piglets deriving from sows vaccinated with China strain. The experimental research included 34 piglets aged 45 days (13 naïve and 21 piglets originating from sows immunized with China strain CSFV). Three experimental groups consisting of seven animals each were formed based on serological examination of piglets aged 40 days. At the age of 45 days, the piglets were vaccinated with a subunit vaccine. After revaccination, the piglets were challenged with a virulent CSFV strain. With the aim of controlling virus shedding, two susceptible piglets were introduced into each group. After challenge, clinical monitoring of animals was performed, and blood samples were obtained to detect viremia and the presence of antibodies against CSF. The control of CSFV shedding by vaccinated, artificially infected piglets was performed by RT-PCR analysis of oropharyngeal and rectal swabs. After death or sacrifice of the animals, autopsy was performed along with the gross pathological examination and tissue sampling with the purpose of determining the presence and distribution of CSFV (ELISA and RT-PCR). Application of the subunit vaccine in piglets originating from sows vaccinated with China-strain resulted in a good active immune response. Following challenge virus shedding was confirmed, but without contact infection in susceptible animals in cohabitation. The results indicate that the subunit vaccine may have a potential application in the control of CSF in enzootic regions.en
dc.description.abstractCilj istraživanja je bio da se utvrdi efikasnost vakcinacije subjedičnom vakcinom protiv infekcije virulentnim virusom KKS kod prasadi poreklom od krmača vakcinisanih Kina sojem. Eksperimenatalna ispitivanja su obavljena na 34 praseta uzrasta 45 dana (13 neimunih i 21 prase poreklom od krmača vakcinisanih Kina sojem). Na osnovu rezultata seroloških ispitivanja prasadi uzrasta 40 dana, formirane su tri eksperimentalne grupe od po sedam prasadi u svakoj. Prasad su vakcinisana subjediničnom vakcinom u uzrastu od 45 dana. Nakon revakcinacije, jedinke su veštački inficirane virulentnim sojem virusa KKS. U cilju kontrole izlučivanja virusa, u svaku eksperimentalnu grupu su dodana po dva neimuna praseta. Nakon veštačke infekcije, vršen je klinički pregled prasadi i uzorkovanje krvi od svake životinje u cilju utvrđivanja prisustva antitela protiv virusa KKS i viremije. Kontrola izlučivanja virusa KKS iz organizma vakcinisanih, veštački inficiranih prasadi je vršena tehnikom RT-PCR na uzorcima orofaringealnih i rektalnih briseva. Nakon uginuća ili žrtvovanja, vršen je patomorfološki pregled i uzorkovanje tkiva u cilju utvrđivanje prisustva i distribucije antigena virusa KKS u uzorcima tkiva (ELISA test i RT-PCR). Vakcinacijom prasadi poreklom od krmača vakcinisanih Kina-sojem utvrđen je solidan aktivni imunski odgovor. Nakon veštačke infekcije utvrđeno je izlučivanje virusa ali bez kontaktne infekcije prijemčivih jedinki u kohabitaciji. Rezultati ukazuju da subjedinična vakcina može imati potencijalnu primenu u kontroli KKS u endemski inficiranim područjima.sr
dc.publisherUniverzitet u Beogradu - Fakultet veterinarske medicine, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31084/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31071/RS//
dc.rightsopenAccess
dc.sourceActa veterinaria - Beograd
dc.subjectclassical swine feveren
dc.subjectcolostral immunityen
dc.subjectsubunit E2 vaccinesen
dc.titleClassical swine fever: Active immunisation of piglets with subunit (E2) vaccine in the presence of different levels of colostral immunity: China strainen
dc.titleKlasična kuga svinja - aktivna imunizacija prasadi subjediničnom (E2) vakcinom u prisustvu različitog nivoa kolostralnog imunitetasr
dc.typearticle
dc.rights.licenseARR
dcterms.abstractРатајац, Радомир; Полачек, Владимир; Дошен, Радослав; Проданов-Радуловић, Јасна; Петровић, Тамаш; Стојанов, Игор; Валчић, Мирослав; Класична куга свиња - активна имунизација прасади субјединичном (Е2) вакцином у присуству различитог нивоа колостралног имунитета; Класична куга свиња - активна имунизација прасади субјединичном (Е2) вакцином у присуству различитог нивоа колостралног имунитета;
dc.citation.volume64
dc.citation.issue4
dc.citation.spage493
dc.citation.epage509
dc.citation.other64(4): 493-509
dc.citation.rankM23
dc.identifier.wos000348509200009
dc.identifier.doi10.2478/acve-2014-0046
dc.identifier.scopus2-s2.0-84921403671
dc.identifier.fulltexthttp://veterinar.vet.bg.ac.rs/bitstream/id/124/1136.pdf
dc.identifier.rcubconv_839
dc.type.versionpublishedVersion


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