Antibiotic resistance and molecular studies on Salmonella enterica subspecies enterica serovar Infantis isolated in human cases and broiler carcasses
Antibiotska rezistencija i molekularna ispitivanja Salmonella enterica subspecies enterica serovar Infantis izolovanih u slučajevima oboljenja ljudi i sa trupova brojlera
2014
Authors
Rašeta, MladenTeodorović, Vlado
Bunčić, Olivera
Katić, Vera
Branković-Lazić, Ivana
Polaček, Vladimir
Vidanović, Dejan
Article (Published version)
Metadata
Show full item recordAbstract
During the year 2012 a study was conducted on the hygiene of the production of broiler carcasses at three abattoirs in the Republic of Serbia. A total of 150 samples of broiler neck skin were examined and 17 salmonella isolates were recorded. Isolates were, by using the corresponding monovalent and polyvalent sera, determined according to type as Salmonella enterica subspecies enterica serovar Infantis (S. Infantis 6, 7, r, 1, 5). In the case of the disease in humans, 5 Salmonella samples of identical serovars were isolated. After that, 22 samples were tested for antibiotic resistance by the disk diffusion test. Isolates showed resistance to ampicillin and nalidixic acid (95.5%), tetracycline (91%), cefotaxime/clavulanic acid (68.2%), but not to ciprofl oxacin, gentamicin, and trimethoprim/sulfamethoxazole. The degree of genetic similarity of isolates from diseased humans and broiler carcasses was determined at a molecular level. Cluster analysis revealed the presence of 7 profiles, wh...ile all isolates have 92% genetic similarity. Although there are differences in the antimicrobial resistance of isolates originating from diseased humans and neck skin of tested broilers, can not be excluded an epidemiological link, because in the dominant genotype SINFXB0001, established in 8 isolates from diseased humans (3 isolates), and the neck skin of broilers (5 isolates), a genetic similarity of 100% was recorded. Based on these results, the presence of S. Infantis on broiler carcasses can be considered a hazard to human health.
Tokom 2012. godine sprovedena su istraživanja higijene procesa proizvodnje trupova brojlera, na tri klanice u Republici Srbiji. Ispitano je 150 uzoraka kožica vrata brojlera i utvrđeno je 17 izolata salmonela. Izolati su, korišćenjem odgovarajućih monovalentnih i polivalentnih seruma, tipizovani kao Salmonella enterica subspecies enterica serovar infantis (S. Infantis 6, 7, r, 1, 5). U slučajevima oboljenja ljudi, izolovano je 5 uzoraka salmonele identičnog serovarijeteta. Nakon toga, na 22 uzorka, rađeno je ispitivanje antibiotske rezistencije izolata disk difuzionim testom. Izolati su pokazali rezistentnost prema: ampicilinu i nalidiksičnoj kiselini (95,5%), tetraciklinu (91%), cefotaksim/klavulanskoj kiselini (68,2%), ali ne i prema ciprofl oksacinu, gentamicinu i trimetoprim/sulfametoksazolu (0%). Stepen genetske sličnosti izolata poreklom od obolelih ljudi i sa trupova brojlera je određen molekularnim metodama. Analiza klastera je pokazala prisustvo 7 profi la, dok svi izolati ima...ju 92% genetske sličnosti. Iako postoje razlike u antimikrobnoj rezistenciji izolata poreklom od obolelih ljudi i sa kožica vrata brojlera, ne može se isključiti epidemiološka povezanost, jer kod dominantnog genotipa SINFXB0001, utvrđenog kod 8 izolata poreklom od obolelih ljudi (3 izolata) i sa kožica vrata brojlera (5 izolata), postoji genetska sličnost od 100%. Na osnovu dobijenih rezultata, istraživanje je pokazalo da prisustvo S. Infantis na trupovima brojlera predstavlja hazard po zdravlje ljudi.
Keywords:
Salmonellosis / prevalence of Salmonella on broiler carcasses / Salmonella Infantis / antibiotic resistance / PFGESource:
Acta Veterinaria-Beograd, 2014, 64, 2, 257-268Publisher:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Funding / projects:
- Improvement and development of hygienic and technological procedures in production of animal originating foodstuffs with the aim of producing high-quality and safe products competetive on the global market (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46009)
DOI: 10.2478/acve-2014-0024
ISSN: 0567-8315
WoS: 000341341200009
Scopus: 2-s2.0-84904996083
Collections
Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Rašeta, Mladen AU - Teodorović, Vlado AU - Bunčić, Olivera AU - Katić, Vera AU - Branković-Lazić, Ivana AU - Polaček, Vladimir AU - Vidanović, Dejan PY - 2014 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1140 AB - During the year 2012 a study was conducted on the hygiene of the production of broiler carcasses at three abattoirs in the Republic of Serbia. A total of 150 samples of broiler neck skin were examined and 17 salmonella isolates were recorded. Isolates were, by using the corresponding monovalent and polyvalent sera, determined according to type as Salmonella enterica subspecies enterica serovar Infantis (S. Infantis 6, 7, r, 1, 5). In the case of the disease in humans, 5 Salmonella samples of identical serovars were isolated. After that, 22 samples were tested for antibiotic resistance by the disk diffusion test. Isolates showed resistance to ampicillin and nalidixic acid (95.5%), tetracycline (91%), cefotaxime/clavulanic acid (68.2%), but not to ciprofl oxacin, gentamicin, and trimethoprim/sulfamethoxazole. The degree of genetic similarity of isolates from diseased humans and broiler carcasses was determined at a molecular level. Cluster analysis revealed the presence of 7 profiles, while all isolates have 92% genetic similarity. Although there are differences in the antimicrobial resistance of isolates originating from diseased humans and neck skin of tested broilers, can not be excluded an epidemiological link, because in the dominant genotype SINFXB0001, established in 8 isolates from diseased humans (3 isolates), and the neck skin of broilers (5 isolates), a genetic similarity of 100% was recorded. Based on these results, the presence of S. Infantis on broiler carcasses can be considered a hazard to human health. AB - Tokom 2012. godine sprovedena su istraživanja higijene procesa proizvodnje trupova brojlera, na tri klanice u Republici Srbiji. Ispitano je 150 uzoraka kožica vrata brojlera i utvrđeno je 17 izolata salmonela. Izolati su, korišćenjem odgovarajućih monovalentnih i polivalentnih seruma, tipizovani kao Salmonella enterica subspecies enterica serovar infantis (S. Infantis 6, 7, r, 1, 5). U slučajevima oboljenja ljudi, izolovano je 5 uzoraka salmonele identičnog serovarijeteta. Nakon toga, na 22 uzorka, rađeno je ispitivanje antibiotske rezistencije izolata disk difuzionim testom. Izolati su pokazali rezistentnost prema: ampicilinu i nalidiksičnoj kiselini (95,5%), tetraciklinu (91%), cefotaksim/klavulanskoj kiselini (68,2%), ali ne i prema ciprofl oksacinu, gentamicinu i trimetoprim/sulfametoksazolu (0%). Stepen genetske sličnosti izolata poreklom od obolelih ljudi i sa trupova brojlera je određen molekularnim metodama. Analiza klastera je pokazala prisustvo 7 profi la, dok svi izolati imaju 92% genetske sličnosti. Iako postoje razlike u antimikrobnoj rezistenciji izolata poreklom od obolelih ljudi i sa kožica vrata brojlera, ne može se isključiti epidemiološka povezanost, jer kod dominantnog genotipa SINFXB0001, utvrđenog kod 8 izolata poreklom od obolelih ljudi (3 izolata) i sa kožica vrata brojlera (5 izolata), postoji genetska sličnost od 100%. Na osnovu dobijenih rezultata, istraživanje je pokazalo da prisustvo S. Infantis na trupovima brojlera predstavlja hazard po zdravlje ljudi. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Antibiotic resistance and molecular studies on Salmonella enterica subspecies enterica serovar Infantis isolated in human cases and broiler carcasses T1 - Antibiotska rezistencija i molekularna ispitivanja Salmonella enterica subspecies enterica serovar Infantis izolovanih u slučajevima oboljenja ljudi i sa trupova brojlera VL - 64 IS - 2 SP - 257 EP - 268 DO - 10.2478/acve-2014-0024 ER -
@article{ author = "Rašeta, Mladen and Teodorović, Vlado and Bunčić, Olivera and Katić, Vera and Branković-Lazić, Ivana and Polaček, Vladimir and Vidanović, Dejan", year = "2014", abstract = "During the year 2012 a study was conducted on the hygiene of the production of broiler carcasses at three abattoirs in the Republic of Serbia. A total of 150 samples of broiler neck skin were examined and 17 salmonella isolates were recorded. Isolates were, by using the corresponding monovalent and polyvalent sera, determined according to type as Salmonella enterica subspecies enterica serovar Infantis (S. Infantis 6, 7, r, 1, 5). In the case of the disease in humans, 5 Salmonella samples of identical serovars were isolated. After that, 22 samples were tested for antibiotic resistance by the disk diffusion test. Isolates showed resistance to ampicillin and nalidixic acid (95.5%), tetracycline (91%), cefotaxime/clavulanic acid (68.2%), but not to ciprofl oxacin, gentamicin, and trimethoprim/sulfamethoxazole. The degree of genetic similarity of isolates from diseased humans and broiler carcasses was determined at a molecular level. Cluster analysis revealed the presence of 7 profiles, while all isolates have 92% genetic similarity. Although there are differences in the antimicrobial resistance of isolates originating from diseased humans and neck skin of tested broilers, can not be excluded an epidemiological link, because in the dominant genotype SINFXB0001, established in 8 isolates from diseased humans (3 isolates), and the neck skin of broilers (5 isolates), a genetic similarity of 100% was recorded. Based on these results, the presence of S. Infantis on broiler carcasses can be considered a hazard to human health., Tokom 2012. godine sprovedena su istraživanja higijene procesa proizvodnje trupova brojlera, na tri klanice u Republici Srbiji. Ispitano je 150 uzoraka kožica vrata brojlera i utvrđeno je 17 izolata salmonela. Izolati su, korišćenjem odgovarajućih monovalentnih i polivalentnih seruma, tipizovani kao Salmonella enterica subspecies enterica serovar infantis (S. Infantis 6, 7, r, 1, 5). U slučajevima oboljenja ljudi, izolovano je 5 uzoraka salmonele identičnog serovarijeteta. Nakon toga, na 22 uzorka, rađeno je ispitivanje antibiotske rezistencije izolata disk difuzionim testom. Izolati su pokazali rezistentnost prema: ampicilinu i nalidiksičnoj kiselini (95,5%), tetraciklinu (91%), cefotaksim/klavulanskoj kiselini (68,2%), ali ne i prema ciprofl oksacinu, gentamicinu i trimetoprim/sulfametoksazolu (0%). Stepen genetske sličnosti izolata poreklom od obolelih ljudi i sa trupova brojlera je određen molekularnim metodama. Analiza klastera je pokazala prisustvo 7 profi la, dok svi izolati imaju 92% genetske sličnosti. Iako postoje razlike u antimikrobnoj rezistenciji izolata poreklom od obolelih ljudi i sa kožica vrata brojlera, ne može se isključiti epidemiološka povezanost, jer kod dominantnog genotipa SINFXB0001, utvrđenog kod 8 izolata poreklom od obolelih ljudi (3 izolata) i sa kožica vrata brojlera (5 izolata), postoji genetska sličnost od 100%. Na osnovu dobijenih rezultata, istraživanje je pokazalo da prisustvo S. Infantis na trupovima brojlera predstavlja hazard po zdravlje ljudi.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Antibiotic resistance and molecular studies on Salmonella enterica subspecies enterica serovar Infantis isolated in human cases and broiler carcasses, Antibiotska rezistencija i molekularna ispitivanja Salmonella enterica subspecies enterica serovar Infantis izolovanih u slučajevima oboljenja ljudi i sa trupova brojlera", volume = "64", number = "2", pages = "257-268", doi = "10.2478/acve-2014-0024" }
Rašeta, M., Teodorović, V., Bunčić, O., Katić, V., Branković-Lazić, I., Polaček, V.,& Vidanović, D.. (2014). Antibiotic resistance and molecular studies on Salmonella enterica subspecies enterica serovar Infantis isolated in human cases and broiler carcasses. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 64(2), 257-268. https://doi.org/10.2478/acve-2014-0024
Rašeta M, Teodorović V, Bunčić O, Katić V, Branković-Lazić I, Polaček V, Vidanović D. Antibiotic resistance and molecular studies on Salmonella enterica subspecies enterica serovar Infantis isolated in human cases and broiler carcasses. in Acta Veterinaria-Beograd. 2014;64(2):257-268. doi:10.2478/acve-2014-0024 .
Rašeta, Mladen, Teodorović, Vlado, Bunčić, Olivera, Katić, Vera, Branković-Lazić, Ivana, Polaček, Vladimir, Vidanović, Dejan, "Antibiotic resistance and molecular studies on Salmonella enterica subspecies enterica serovar Infantis isolated in human cases and broiler carcasses" in Acta Veterinaria-Beograd, 64, no. 2 (2014):257-268, https://doi.org/10.2478/acve-2014-0024 . .