A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from Serbia using real-time RT-PCR
Ispitivanje prisustva virusa deformacije krila i virusa akutne paralize pčela u pčelinjim zajednicama iz Srbije upotrebom 'real-time RT-PCR' metode
2014
Аутори
Simeunović, PredragStevanović, Jevrosima
Vidanović, Dejan
Nišavić, Jakov
Radović, Dejan
Stanišić, Ljubodrag
Stanimirović, Zoran
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In this study 55 honey bee colonies from different Serbian regions were monitored for the presence of Deformed Wing Virus (DWV) and Acute Bee Paralysis Virus (ABPV) using TaqMan-based real-time RT-PCR assay. The results revealed the presence of DWV in each sampling location, and ABPV in 10 out of 11 apiaries. High frequency of DWV (76.4%) and ABPV (61.8%) positive samples in asymptomatic colonies can be the consequence of inefficient and postponed Varroa treatment concerning the role of this mite in the transmission and activation of honey bee viruses. The real-time RTPCR technique described in this paper is proved to be the most reliable method for this kind of investigation.
Ovo istraživanje je obuhvatilo utvrđivanje prisustva virusa deformacije krila (DWV) i virusa akutne paralize pčela (ABPV) u 55 pčelinjih društava poreklom iz različitih delova teritorije Srbije, upotrebom 'real-time RT-PCR' metode bazirane na 'TaqMan' probama. Rezultati pokazuju prisustvo DWV na svakom ispitivanom lokalitetu, dok je ABPV bio prisutan u 10 od 11 ispitivanih pčelinjaka. Imajući u vidu ulogu ektoparazita Varroa destructor u prenošenju i aktivaciji pčelinjih virusa, visoka učestalost uzoraka pozitivnih na DWV (76,4%) i ABPV (61,8%) u asimptomatskim pčelinjim društvima može biti posledica nedovoljno efikasnog i zakasnelog tretmana protiv ove grinje. 'Realtime RT-PCR' metoda, opisana u ovom radu se pokazala kao najpogodnija metoda za ovakvu vrstu istraživanja.
Кључне речи:
DWV / ABPV / Varroa destructor / Serbian apiariesИзвор:
Acta Veterinaria-Beograd, 2014, 64, 1, 81-92Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Финансирање / пројекти:
- Молекуларно-генетичка и екофизиолошка истраживања у заштити аутохтоних анималних генетичких ресурса, очувању добробити, здравља и репродукције гајених животиња и производњи безбедне хране (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
DOI: 10.2478/acve-2014-0009
ISSN: 0567-8315
WoS: 000341341000009
Scopus: 2-s2.0-84902511509
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Simeunović, Predrag AU - Stevanović, Jevrosima AU - Vidanović, Dejan AU - Nišavić, Jakov AU - Radović, Dejan AU - Stanišić, Ljubodrag AU - Stanimirović, Zoran PY - 2014 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1144 AB - In this study 55 honey bee colonies from different Serbian regions were monitored for the presence of Deformed Wing Virus (DWV) and Acute Bee Paralysis Virus (ABPV) using TaqMan-based real-time RT-PCR assay. The results revealed the presence of DWV in each sampling location, and ABPV in 10 out of 11 apiaries. High frequency of DWV (76.4%) and ABPV (61.8%) positive samples in asymptomatic colonies can be the consequence of inefficient and postponed Varroa treatment concerning the role of this mite in the transmission and activation of honey bee viruses. The real-time RTPCR technique described in this paper is proved to be the most reliable method for this kind of investigation. AB - Ovo istraživanje je obuhvatilo utvrđivanje prisustva virusa deformacije krila (DWV) i virusa akutne paralize pčela (ABPV) u 55 pčelinjih društava poreklom iz različitih delova teritorije Srbije, upotrebom 'real-time RT-PCR' metode bazirane na 'TaqMan' probama. Rezultati pokazuju prisustvo DWV na svakom ispitivanom lokalitetu, dok je ABPV bio prisutan u 10 od 11 ispitivanih pčelinjaka. Imajući u vidu ulogu ektoparazita Varroa destructor u prenošenju i aktivaciji pčelinjih virusa, visoka učestalost uzoraka pozitivnih na DWV (76,4%) i ABPV (61,8%) u asimptomatskim pčelinjim društvima može biti posledica nedovoljno efikasnog i zakasnelog tretmana protiv ove grinje. 'Realtime RT-PCR' metoda, opisana u ovom radu se pokazala kao najpogodnija metoda za ovakvu vrstu istraživanja. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from Serbia using real-time RT-PCR T1 - Ispitivanje prisustva virusa deformacije krila i virusa akutne paralize pčela u pčelinjim zajednicama iz Srbije upotrebom 'real-time RT-PCR' metode VL - 64 IS - 1 SP - 81 EP - 92 DO - 10.2478/acve-2014-0009 ER -
@article{ author = "Simeunović, Predrag and Stevanović, Jevrosima and Vidanović, Dejan and Nišavić, Jakov and Radović, Dejan and Stanišić, Ljubodrag and Stanimirović, Zoran", year = "2014", abstract = "In this study 55 honey bee colonies from different Serbian regions were monitored for the presence of Deformed Wing Virus (DWV) and Acute Bee Paralysis Virus (ABPV) using TaqMan-based real-time RT-PCR assay. The results revealed the presence of DWV in each sampling location, and ABPV in 10 out of 11 apiaries. High frequency of DWV (76.4%) and ABPV (61.8%) positive samples in asymptomatic colonies can be the consequence of inefficient and postponed Varroa treatment concerning the role of this mite in the transmission and activation of honey bee viruses. The real-time RTPCR technique described in this paper is proved to be the most reliable method for this kind of investigation., Ovo istraživanje je obuhvatilo utvrđivanje prisustva virusa deformacije krila (DWV) i virusa akutne paralize pčela (ABPV) u 55 pčelinjih društava poreklom iz različitih delova teritorije Srbije, upotrebom 'real-time RT-PCR' metode bazirane na 'TaqMan' probama. Rezultati pokazuju prisustvo DWV na svakom ispitivanom lokalitetu, dok je ABPV bio prisutan u 10 od 11 ispitivanih pčelinjaka. Imajući u vidu ulogu ektoparazita Varroa destructor u prenošenju i aktivaciji pčelinjih virusa, visoka učestalost uzoraka pozitivnih na DWV (76,4%) i ABPV (61,8%) u asimptomatskim pčelinjim društvima može biti posledica nedovoljno efikasnog i zakasnelog tretmana protiv ove grinje. 'Realtime RT-PCR' metoda, opisana u ovom radu se pokazala kao najpogodnija metoda za ovakvu vrstu istraživanja.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from Serbia using real-time RT-PCR, Ispitivanje prisustva virusa deformacije krila i virusa akutne paralize pčela u pčelinjim zajednicama iz Srbije upotrebom 'real-time RT-PCR' metode", volume = "64", number = "1", pages = "81-92", doi = "10.2478/acve-2014-0009" }
Simeunović, P., Stevanović, J., Vidanović, D., Nišavić, J., Radović, D., Stanišić, L.,& Stanimirović, Z.. (2014). A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from Serbia using real-time RT-PCR. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 64(1), 81-92. https://doi.org/10.2478/acve-2014-0009
Simeunović P, Stevanović J, Vidanović D, Nišavić J, Radović D, Stanišić L, Stanimirović Z. A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from Serbia using real-time RT-PCR. in Acta Veterinaria-Beograd. 2014;64(1):81-92. doi:10.2478/acve-2014-0009 .
Simeunović, Predrag, Stevanović, Jevrosima, Vidanović, Dejan, Nišavić, Jakov, Radović, Dejan, Stanišić, Ljubodrag, Stanimirović, Zoran, "A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from Serbia using real-time RT-PCR" in Acta Veterinaria-Beograd, 64, no. 1 (2014):81-92, https://doi.org/10.2478/acve-2014-0009 . .