Nosema ceranae DNA in honey bee haemolymph and honey bee mite Varroa destructor
DNK Nosema ceranae u hemolimfi pčela i pčelinjem krpelju Varroa destructor
2014
Authors
Glavinić, UrošStevanović, Jevrosima
Gajić, Bojan
Simeunović, Predrag
Đurić, Spomenka
Vejnović, Branislav
Stanimirović, Zoran
Article (Published version)
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Honey bee mite Varroa destructor and microsporidium Nosema ceranae are currently considered the most important threats to honey bees and beekeeping. It has been believed that both N. apis and N. ceranae invade exclusively epithelial cells of the honey bee ventriculus. However, some findings suggest that these microsporidia may infect other tissues of honey bees. There are indications that these pathogens could be found in honey bee haemolymph, as the medium for its distribution to anatomically distant tissues. Knowing that V. destructor being an ectoparasitic mite feeds on the honey bee's haemolymph, the aim of this study was to investigate if DNA of Nosema spp. microsporidia could be found in honey bee haemolymph and in V. destructor. The study was conducted on bee haemolymph and V. destructor mites from 44 Apis mellifera colonies. From each hive five mite individuals and 10 μL of haemolymph (from 4-5 bees) were used as samples for DNA isolation and PCR detection of Nosema spp. The DN...A of N. ceranae was confirmed in 61.36% of V. destructor mites and 68.18% of haemolymph samples. This is the first report of N. ceranae DNA in honey bee haemolymph and in V. destructor mites. The finding of DNA of N. ceranae in V. destructor could be interpreted as the result of mite feeding on N. ceranae infected bee haemolymph. However, for a full confirmation of the vector role of V. destructor in spreading of nosemosis, further microscopy investigations are required for the detection of spores in both investigated matrices (haemolymph and V. destructor internal tissues).
Pčelinji krpelj Varroa destructor i mikrosporidija Nosema ceranae su najznačajniji faktori rizika po zdravlje pčela i pčelarstvo. Dugo se smatralo da N. apis i N. ceranae inficiraju isključivo ćelije crevnog epitela pčela, međutim neki nalazi ukazuju na prisustvo ovih mikrosporidija i u drugim tkivima. Postoje indikacije da se ovi patogeni mogu naći i u hemolimfi pčela, kao medijumu za njihovu distribuciju do anatomski udaljenih tkiva. S obzirom na to da se V. destructor kao ektoparazit hrani hemolimfom pčela, cilj ovog rada bio je ispitivanje prisustva DNK mikrosporidija roda Nosema u hemolimfi pčela i pčelinjem krpelju V. destructor. Istraživanje je obavljeno na uzorcima pčelinje hemolimfe i pčelinjim krpeljima V. destructor iz 44 društva Apis mellifera. Iz svake košnice je uzorkovano pet krpelja i 10 μL hemolimfe (sakupljene iz 4-5 pčela) koji su korišćeni kao uzorci za izolaciju DNK i ispitivanje prisustva Nosema spp. putem PCR metode. Prisustvo DNK N. ceranae je potvrđeno u 61,36%... analiziranih krpelja V. destructor i 68,18% uzoraka hemolimfe pčela, što predstavlja prvu detekciju prisustva DNK N. ceranae u hemolimfi pčela i krpeljima V. destructor. Nalaz DNK N. ceranae u V. destructor može se tumačiti kao rezultat ishrane V. destructor hemolimfom pčela koja je inficirana sa N. ceranae. Međutim, da bi se potvrdila vektorska uloga V. destructor u širenju nozemoze, potrebna su dalja mikroskopska istraživanja radi detekcije spora u oba ispitivana medijuma (hemolimfi i unutrašnjim tkivima V. destructor krpelja).
Keywords:
Nosema ceranae / Varroa destructor / haemolymph / Apis mellifera / PCRSource:
Acta Veterinaria-Beograd, 2014, 64, 3, 349-357Publisher:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Funding / projects:
- Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
DOI: 10.2478/acve-2014-0033
ISSN: 0567-8315
WoS: 000343403500007
Scopus: 2-s2.0-84907690709
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Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Glavinić, Uroš AU - Stevanović, Jevrosima AU - Gajić, Bojan AU - Simeunović, Predrag AU - Đurić, Spomenka AU - Vejnović, Branislav AU - Stanimirović, Zoran PY - 2014 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1148 AB - Honey bee mite Varroa destructor and microsporidium Nosema ceranae are currently considered the most important threats to honey bees and beekeeping. It has been believed that both N. apis and N. ceranae invade exclusively epithelial cells of the honey bee ventriculus. However, some findings suggest that these microsporidia may infect other tissues of honey bees. There are indications that these pathogens could be found in honey bee haemolymph, as the medium for its distribution to anatomically distant tissues. Knowing that V. destructor being an ectoparasitic mite feeds on the honey bee's haemolymph, the aim of this study was to investigate if DNA of Nosema spp. microsporidia could be found in honey bee haemolymph and in V. destructor. The study was conducted on bee haemolymph and V. destructor mites from 44 Apis mellifera colonies. From each hive five mite individuals and 10 μL of haemolymph (from 4-5 bees) were used as samples for DNA isolation and PCR detection of Nosema spp. The DNA of N. ceranae was confirmed in 61.36% of V. destructor mites and 68.18% of haemolymph samples. This is the first report of N. ceranae DNA in honey bee haemolymph and in V. destructor mites. The finding of DNA of N. ceranae in V. destructor could be interpreted as the result of mite feeding on N. ceranae infected bee haemolymph. However, for a full confirmation of the vector role of V. destructor in spreading of nosemosis, further microscopy investigations are required for the detection of spores in both investigated matrices (haemolymph and V. destructor internal tissues). AB - Pčelinji krpelj Varroa destructor i mikrosporidija Nosema ceranae su najznačajniji faktori rizika po zdravlje pčela i pčelarstvo. Dugo se smatralo da N. apis i N. ceranae inficiraju isključivo ćelije crevnog epitela pčela, međutim neki nalazi ukazuju na prisustvo ovih mikrosporidija i u drugim tkivima. Postoje indikacije da se ovi patogeni mogu naći i u hemolimfi pčela, kao medijumu za njihovu distribuciju do anatomski udaljenih tkiva. S obzirom na to da se V. destructor kao ektoparazit hrani hemolimfom pčela, cilj ovog rada bio je ispitivanje prisustva DNK mikrosporidija roda Nosema u hemolimfi pčela i pčelinjem krpelju V. destructor. Istraživanje je obavljeno na uzorcima pčelinje hemolimfe i pčelinjim krpeljima V. destructor iz 44 društva Apis mellifera. Iz svake košnice je uzorkovano pet krpelja i 10 μL hemolimfe (sakupljene iz 4-5 pčela) koji su korišćeni kao uzorci za izolaciju DNK i ispitivanje prisustva Nosema spp. putem PCR metode. Prisustvo DNK N. ceranae je potvrđeno u 61,36% analiziranih krpelja V. destructor i 68,18% uzoraka hemolimfe pčela, što predstavlja prvu detekciju prisustva DNK N. ceranae u hemolimfi pčela i krpeljima V. destructor. Nalaz DNK N. ceranae u V. destructor može se tumačiti kao rezultat ishrane V. destructor hemolimfom pčela koja je inficirana sa N. ceranae. Međutim, da bi se potvrdila vektorska uloga V. destructor u širenju nozemoze, potrebna su dalja mikroskopska istraživanja radi detekcije spora u oba ispitivana medijuma (hemolimfi i unutrašnjim tkivima V. destructor krpelja). PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Nosema ceranae DNA in honey bee haemolymph and honey bee mite Varroa destructor T1 - DNK Nosema ceranae u hemolimfi pčela i pčelinjem krpelju Varroa destructor VL - 64 IS - 3 SP - 349 EP - 357 DO - 10.2478/acve-2014-0033 ER -
@article{ author = "Glavinić, Uroš and Stevanović, Jevrosima and Gajić, Bojan and Simeunović, Predrag and Đurić, Spomenka and Vejnović, Branislav and Stanimirović, Zoran", year = "2014", abstract = "Honey bee mite Varroa destructor and microsporidium Nosema ceranae are currently considered the most important threats to honey bees and beekeeping. It has been believed that both N. apis and N. ceranae invade exclusively epithelial cells of the honey bee ventriculus. However, some findings suggest that these microsporidia may infect other tissues of honey bees. There are indications that these pathogens could be found in honey bee haemolymph, as the medium for its distribution to anatomically distant tissues. Knowing that V. destructor being an ectoparasitic mite feeds on the honey bee's haemolymph, the aim of this study was to investigate if DNA of Nosema spp. microsporidia could be found in honey bee haemolymph and in V. destructor. The study was conducted on bee haemolymph and V. destructor mites from 44 Apis mellifera colonies. From each hive five mite individuals and 10 μL of haemolymph (from 4-5 bees) were used as samples for DNA isolation and PCR detection of Nosema spp. The DNA of N. ceranae was confirmed in 61.36% of V. destructor mites and 68.18% of haemolymph samples. This is the first report of N. ceranae DNA in honey bee haemolymph and in V. destructor mites. The finding of DNA of N. ceranae in V. destructor could be interpreted as the result of mite feeding on N. ceranae infected bee haemolymph. However, for a full confirmation of the vector role of V. destructor in spreading of nosemosis, further microscopy investigations are required for the detection of spores in both investigated matrices (haemolymph and V. destructor internal tissues)., Pčelinji krpelj Varroa destructor i mikrosporidija Nosema ceranae su najznačajniji faktori rizika po zdravlje pčela i pčelarstvo. Dugo se smatralo da N. apis i N. ceranae inficiraju isključivo ćelije crevnog epitela pčela, međutim neki nalazi ukazuju na prisustvo ovih mikrosporidija i u drugim tkivima. Postoje indikacije da se ovi patogeni mogu naći i u hemolimfi pčela, kao medijumu za njihovu distribuciju do anatomski udaljenih tkiva. S obzirom na to da se V. destructor kao ektoparazit hrani hemolimfom pčela, cilj ovog rada bio je ispitivanje prisustva DNK mikrosporidija roda Nosema u hemolimfi pčela i pčelinjem krpelju V. destructor. Istraživanje je obavljeno na uzorcima pčelinje hemolimfe i pčelinjim krpeljima V. destructor iz 44 društva Apis mellifera. Iz svake košnice je uzorkovano pet krpelja i 10 μL hemolimfe (sakupljene iz 4-5 pčela) koji su korišćeni kao uzorci za izolaciju DNK i ispitivanje prisustva Nosema spp. putem PCR metode. Prisustvo DNK N. ceranae je potvrđeno u 61,36% analiziranih krpelja V. destructor i 68,18% uzoraka hemolimfe pčela, što predstavlja prvu detekciju prisustva DNK N. ceranae u hemolimfi pčela i krpeljima V. destructor. Nalaz DNK N. ceranae u V. destructor može se tumačiti kao rezultat ishrane V. destructor hemolimfom pčela koja je inficirana sa N. ceranae. Međutim, da bi se potvrdila vektorska uloga V. destructor u širenju nozemoze, potrebna su dalja mikroskopska istraživanja radi detekcije spora u oba ispitivana medijuma (hemolimfi i unutrašnjim tkivima V. destructor krpelja).", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Nosema ceranae DNA in honey bee haemolymph and honey bee mite Varroa destructor, DNK Nosema ceranae u hemolimfi pčela i pčelinjem krpelju Varroa destructor", volume = "64", number = "3", pages = "349-357", doi = "10.2478/acve-2014-0033" }
Glavinić, U., Stevanović, J., Gajić, B., Simeunović, P., Đurić, S., Vejnović, B.,& Stanimirović, Z.. (2014). Nosema ceranae DNA in honey bee haemolymph and honey bee mite Varroa destructor. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 64(3), 349-357. https://doi.org/10.2478/acve-2014-0033
Glavinić U, Stevanović J, Gajić B, Simeunović P, Đurić S, Vejnović B, Stanimirović Z. Nosema ceranae DNA in honey bee haemolymph and honey bee mite Varroa destructor. in Acta Veterinaria-Beograd. 2014;64(3):349-357. doi:10.2478/acve-2014-0033 .
Glavinić, Uroš, Stevanović, Jevrosima, Gajić, Bojan, Simeunović, Predrag, Đurić, Spomenka, Vejnović, Branislav, Stanimirović, Zoran, "Nosema ceranae DNA in honey bee haemolymph and honey bee mite Varroa destructor" in Acta Veterinaria-Beograd, 64, no. 3 (2014):349-357, https://doi.org/10.2478/acve-2014-0033 . .