Show simple item record

DNK Nosema ceranae u hemolimfi pčela i pčelinjem krpelju Varroa destructor

dc.creatorGlavinić, Uroš
dc.creatorStevanović, Jevrosima
dc.creatorGajić, Bojan
dc.creatorSimeunović, Predrag
dc.creatorĐurić, Spomenka
dc.creatorVejnović, Branislav
dc.creatorStanimirović, Zoran
dc.date.accessioned2020-06-03T13:51:44Z
dc.date.available2020-06-03T13:51:44Z
dc.date.issued2014
dc.identifier.issn0567-8315
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1148
dc.description.abstractHoney bee mite Varroa destructor and microsporidium Nosema ceranae are currently considered the most important threats to honey bees and beekeeping. It has been believed that both N. apis and N. ceranae invade exclusively epithelial cells of the honey bee ventriculus. However, some findings suggest that these microsporidia may infect other tissues of honey bees. There are indications that these pathogens could be found in honey bee haemolymph, as the medium for its distribution to anatomically distant tissues. Knowing that V. destructor being an ectoparasitic mite feeds on the honey bee's haemolymph, the aim of this study was to investigate if DNA of Nosema spp. microsporidia could be found in honey bee haemolymph and in V. destructor. The study was conducted on bee haemolymph and V. destructor mites from 44 Apis mellifera colonies. From each hive five mite individuals and 10 μL of haemolymph (from 4-5 bees) were used as samples for DNA isolation and PCR detection of Nosema spp. The DNA of N. ceranae was confirmed in 61.36% of V. destructor mites and 68.18% of haemolymph samples. This is the first report of N. ceranae DNA in honey bee haemolymph and in V. destructor mites. The finding of DNA of N. ceranae in V. destructor could be interpreted as the result of mite feeding on N. ceranae infected bee haemolymph. However, for a full confirmation of the vector role of V. destructor in spreading of nosemosis, further microscopy investigations are required for the detection of spores in both investigated matrices (haemolymph and V. destructor internal tissues).en
dc.description.abstractPčelinji krpelj Varroa destructor i mikrosporidija Nosema ceranae su najznačajniji faktori rizika po zdravlje pčela i pčelarstvo. Dugo se smatralo da N. apis i N. ceranae inficiraju isključivo ćelije crevnog epitela pčela, međutim neki nalazi ukazuju na prisustvo ovih mikrosporidija i u drugim tkivima. Postoje indikacije da se ovi patogeni mogu naći i u hemolimfi pčela, kao medijumu za njihovu distribuciju do anatomski udaljenih tkiva. S obzirom na to da se V. destructor kao ektoparazit hrani hemolimfom pčela, cilj ovog rada bio je ispitivanje prisustva DNK mikrosporidija roda Nosema u hemolimfi pčela i pčelinjem krpelju V. destructor. Istraživanje je obavljeno na uzorcima pčelinje hemolimfe i pčelinjim krpeljima V. destructor iz 44 društva Apis mellifera. Iz svake košnice je uzorkovano pet krpelja i 10 μL hemolimfe (sakupljene iz 4-5 pčela) koji su korišćeni kao uzorci za izolaciju DNK i ispitivanje prisustva Nosema spp. putem PCR metode. Prisustvo DNK N. ceranae je potvrđeno u 61,36% analiziranih krpelja V. destructor i 68,18% uzoraka hemolimfe pčela, što predstavlja prvu detekciju prisustva DNK N. ceranae u hemolimfi pčela i krpeljima V. destructor. Nalaz DNK N. ceranae u V. destructor može se tumačiti kao rezultat ishrane V. destructor hemolimfom pčela koja je inficirana sa N. ceranae. Međutim, da bi se potvrdila vektorska uloga V. destructor u širenju nozemoze, potrebna su dalja mikroskopska istraživanja radi detekcije spora u oba ispitivana medijuma (hemolimfi i unutrašnjim tkivima V. destructor krpelja).sr
dc.publisherUniverzitet u Beogradu - Fakultet veterinarske medicine, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46002/RS//
dc.rightsopenAccess
dc.sourceActa veterinaria - Beograd
dc.subjectNosema ceranaeen
dc.subjectVarroa destructoren
dc.subjecthaemolymphen
dc.subjectApis melliferaen
dc.subjectPCRen
dc.titleNosema ceranae DNA in honey bee haemolymph and honey bee mite Varroa destructoren
dc.titleDNK Nosema ceranae u hemolimfi pčela i pčelinjem krpelju Varroa destructorsr
dc.typearticle
dc.rights.licenseARR
dcterms.abstractСтанимировић, Зоран; Гајић, Бојан; Симеуновић, Предраг; Главинић, Урош; Стевановић, Јевросима; Вејновић, Бранислав; Ђурић, Споменка; ДНК Носема церанае у хемолимфи пчела и пчелињем крпељу Варроа деструцтор; ДНК Носема церанае у хемолимфи пчела и пчелињем крпељу Варроа деструцтор;
dc.citation.volume64
dc.citation.issue3
dc.citation.spage349
dc.citation.epage357
dc.citation.other64(3): 349-357
dc.citation.rankM23
dc.identifier.wos000343403500007
dc.identifier.doi10.2478/acve-2014-0033
dc.identifier.scopus2-s2.0-84907690709
dc.identifier.fulltexthttp://veterinar.vet.bg.ac.rs/bitstream/id/134/1147.pdf
dc.identifier.rcubconv_833
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record