Show simple item record

dc.creatorKovačević, Milica
dc.creatorBozić, T
dc.creatorIvanović, Z
dc.date.accessioned2020-06-03T12:39:29Z
dc.date.available2020-06-03T12:39:29Z
dc.date.issued1999
dc.identifier.issn1090-0233
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/116
dc.description.abstractWith the intention of using the pig as a large animal model in haematopoietic research, a clonal assay in methylcellulose was developed and the optimal conditions for raising erythroid progenitors from adult pig bone marrow (BM) and peripheral blood (PB) have been established. Progenitor cells were stimulated to proliferate and differentiate in vitro by growth factors containing leucocyte condition medium (LCM), and with recombinant human erythropoietin (rhEpo). The number of PB BFU-E (burst forming units - erythroid) directly depended on the concentration of LCM, but BM BFU-E were not dependent on LCM. Both CFU-E (colony forming units - erythroid) and BFU-E were rhEpo, dependent. Despite relatively high but expected individual variations, the mean number of colonies, as well as the functional characteristics of progenitor cells investigated, were similar to those of miniature pigs and some other mammals.en
dc.publisherElsevier Sci Ltd, Oxford
dc.rightsrestrictedAccess
dc.sourceVeterinary Journal
dc.subjecterythropoietic progenitorsen
dc.subjectbone marrowen
dc.subjectperipheral blooden
dc.subjectpigen
dc.subjectin vitroen
dc.titleErythroid progenitor cells from pig bone marrow and peripheral blooden
dc.typearticle
dc.rights.licenseARR
dcterms.abstractКовачевић, Милица; Ивановић, З; Бозић, Т;
dc.citation.volume158
dc.citation.issue3
dc.citation.spage196
dc.citation.epage203
dc.citation.other158(3): 196-203
dc.citation.rankM21
dc.identifier.wos000083807600006
dc.identifier.doi10.1053/tvjl.1999.0382
dc.identifier.pmid10558839
dc.identifier.scopus2-s2.0-0033227040
dc.identifier.rcubconv_1320
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record