Proliferation and differentiation potential of canine synovial fluid cells
2015
Autori
Francuski, JelenaDebeljak-Martačić, Jasmina
Radovanović, Anita
Andrić, Nenad
Sourice-Petit, Sophie
Guicheux, Jerome
Mojsilović, Slavko
Kovačević-Filipović, Milica
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The aim of this study was to determine whether synovial fluid (SF) of dogs contains cells that have characteristics of MSCs and to describe their differentiation potential. SF adherent cells from 5 young German shepherd dogs (average 3.8 +/- 0.9 years) were expanded (37 degrees C, 5% CO2, humidified atmosphere) three weeks before their phenotype was characterized by flow-cytometry for the presence of CD90 and CD34. Population doubling time (PDT), number of CFU-F and adipogenic, osteogenic and chondrogenic potentials have been determined in vitro. In early passages PTD was 31 +/- 10 hours and expansion fold after 3 sub cultivations (9 days) theoretically could be 372 +/- 134. At P1, 0.55 +/- 0.05% of SF cells had the ability to form CFU-F. Sixty-six percent of cells expressed CD90 and none of the cells expressed markers of hematopoietic cells. Oil Red O staining has shown accumulation of fat droplets in cells grown in adipogenic medium, while deposits of calcium in the osteogenic medium... were evidenced with Alizarin red staining. SF cultured in hondrogenic and control medium in three-dimensional conditions formed a cartilage-like tissue. Alcian blue staining of pellets slides have shown a significant amount of glycosaminoglycans (GAGs) and immunohistochemistry analysis documented collagen type II expression. The amount of GAGs in pellets grown in both conditions showed no difference. SF cells in vitro exhibited osteogenic, adipogenic and chondrogenic differentiation potentials, suggesting the presence of different mesenchymal progenitors. These results also demonstrated that SF cells have a spontaneous chondrogenic potential that should be further explored for possible tissue engineering protocols.
Ključne reči:
chondrogenesis / dog / mesenchymal stem cell / synovial fluidIzvor:
Acta Veterinaria-Beograd, 2015, 65, 1, 66-78Izdavač:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Finansiranje / projekti:
- Antioksidativna zaštita i potencijali za diferencijaciju i regeneraciju mezenhimalnih matičnih ćelija iz različitih tkiva tokom procesa starenja (RS-MESTD-Basic Research (BR or ON)-175061)
- Bilateral Serbian-French Co-operation "Pavle Savic"
- Short Term Scientific Mission COST Action MP1005 - NAMABIO
- ANR Tecsan "Chondrograft"French National Research Agency (ANR)
DOI: 10.1515/acve-2015-0005
ISSN: 0567-8315
WoS: 000351737400005
Scopus: 2-s2.0-84927614465
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Francuski, Jelena AU - Debeljak-Martačić, Jasmina AU - Radovanović, Anita AU - Andrić, Nenad AU - Sourice-Petit, Sophie AU - Guicheux, Jerome AU - Mojsilović, Slavko AU - Kovačević-Filipović, Milica PY - 2015 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1171 AB - The aim of this study was to determine whether synovial fluid (SF) of dogs contains cells that have characteristics of MSCs and to describe their differentiation potential. SF adherent cells from 5 young German shepherd dogs (average 3.8 +/- 0.9 years) were expanded (37 degrees C, 5% CO2, humidified atmosphere) three weeks before their phenotype was characterized by flow-cytometry for the presence of CD90 and CD34. Population doubling time (PDT), number of CFU-F and adipogenic, osteogenic and chondrogenic potentials have been determined in vitro. In early passages PTD was 31 +/- 10 hours and expansion fold after 3 sub cultivations (9 days) theoretically could be 372 +/- 134. At P1, 0.55 +/- 0.05% of SF cells had the ability to form CFU-F. Sixty-six percent of cells expressed CD90 and none of the cells expressed markers of hematopoietic cells. Oil Red O staining has shown accumulation of fat droplets in cells grown in adipogenic medium, while deposits of calcium in the osteogenic medium were evidenced with Alizarin red staining. SF cultured in hondrogenic and control medium in three-dimensional conditions formed a cartilage-like tissue. Alcian blue staining of pellets slides have shown a significant amount of glycosaminoglycans (GAGs) and immunohistochemistry analysis documented collagen type II expression. The amount of GAGs in pellets grown in both conditions showed no difference. SF cells in vitro exhibited osteogenic, adipogenic and chondrogenic differentiation potentials, suggesting the presence of different mesenchymal progenitors. These results also demonstrated that SF cells have a spontaneous chondrogenic potential that should be further explored for possible tissue engineering protocols. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Proliferation and differentiation potential of canine synovial fluid cells VL - 65 IS - 1 SP - 66 EP - 78 DO - 10.1515/acve-2015-0005 ER -
@article{ author = "Francuski, Jelena and Debeljak-Martačić, Jasmina and Radovanović, Anita and Andrić, Nenad and Sourice-Petit, Sophie and Guicheux, Jerome and Mojsilović, Slavko and Kovačević-Filipović, Milica", year = "2015", abstract = "The aim of this study was to determine whether synovial fluid (SF) of dogs contains cells that have characteristics of MSCs and to describe their differentiation potential. SF adherent cells from 5 young German shepherd dogs (average 3.8 +/- 0.9 years) were expanded (37 degrees C, 5% CO2, humidified atmosphere) three weeks before their phenotype was characterized by flow-cytometry for the presence of CD90 and CD34. Population doubling time (PDT), number of CFU-F and adipogenic, osteogenic and chondrogenic potentials have been determined in vitro. In early passages PTD was 31 +/- 10 hours and expansion fold after 3 sub cultivations (9 days) theoretically could be 372 +/- 134. At P1, 0.55 +/- 0.05% of SF cells had the ability to form CFU-F. Sixty-six percent of cells expressed CD90 and none of the cells expressed markers of hematopoietic cells. Oil Red O staining has shown accumulation of fat droplets in cells grown in adipogenic medium, while deposits of calcium in the osteogenic medium were evidenced with Alizarin red staining. SF cultured in hondrogenic and control medium in three-dimensional conditions formed a cartilage-like tissue. Alcian blue staining of pellets slides have shown a significant amount of glycosaminoglycans (GAGs) and immunohistochemistry analysis documented collagen type II expression. The amount of GAGs in pellets grown in both conditions showed no difference. SF cells in vitro exhibited osteogenic, adipogenic and chondrogenic differentiation potentials, suggesting the presence of different mesenchymal progenitors. These results also demonstrated that SF cells have a spontaneous chondrogenic potential that should be further explored for possible tissue engineering protocols.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Proliferation and differentiation potential of canine synovial fluid cells", volume = "65", number = "1", pages = "66-78", doi = "10.1515/acve-2015-0005" }
Francuski, J., Debeljak-Martačić, J., Radovanović, A., Andrić, N., Sourice-Petit, S., Guicheux, J., Mojsilović, S.,& Kovačević-Filipović, M.. (2015). Proliferation and differentiation potential of canine synovial fluid cells. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(1), 66-78. https://doi.org/10.1515/acve-2015-0005
Francuski J, Debeljak-Martačić J, Radovanović A, Andrić N, Sourice-Petit S, Guicheux J, Mojsilović S, Kovačević-Filipović M. Proliferation and differentiation potential of canine synovial fluid cells. in Acta Veterinaria-Beograd. 2015;65(1):66-78. doi:10.1515/acve-2015-0005 .
Francuski, Jelena, Debeljak-Martačić, Jasmina, Radovanović, Anita, Andrić, Nenad, Sourice-Petit, Sophie, Guicheux, Jerome, Mojsilović, Slavko, Kovačević-Filipović, Milica, "Proliferation and differentiation potential of canine synovial fluid cells" in Acta Veterinaria-Beograd, 65, no. 1 (2015):66-78, https://doi.org/10.1515/acve-2015-0005 . .