Detection of equine arteritis virus in the semen of stallions in the republic of Serbia
2015
Authors
Lazić, SavaLupulović, Diana
Polaček, Vladimir
Valčić, Miroslav
Lazić, Gospava
Pasagić, Enisa
Petrović, Tamaš
Article (Published version)
Metadata
Show full item recordAbstract
The results on serological testing of blood sera from stallions and mares used for breeding and the presence of the viral genome of Equine Arteritis Virus (EAV) in stallion semen are presented. The blood and semen samples were taken from a horse stable on the territory of the Republic of Serbia during 2012, 2013 and 2014. Detection of anti-EAV specific antibodies in blood sera was performed by the virus neutralization test (VNT), and identification of EAV genome RNA in stallion semen was done by reverse-transcriptase polymerase chain reaction (RT-PCR). In 2012, high seroprevalence of EAV was detected in the investigated stable. In total, 45% and 65 % of stallions and mares reacted positive, respectively, and the antibody titre values ranged between 2 and 10 log 2. High seroprevalence was confirmed in the same animals again in 2013. Out of two stallions tested semen samples in 2013, the viral genome was detected by RT-PCR in 3 examined semen samples from a seropositive stallion, while E...AV was not detected in 3 semen samples of a seronegative stallion. During 2014, 11 semen samples were collected from two seropositive stallions. Again, the presence of EAV was confirmed by RT-PCR in all 8 semen samples originating from the same stallion with the EAV genome positive semen result in 2013, whereas the virus was not detected in semen samples originating from the second anti-EAV antibody positive stallion. The presence of EAV-specific antibodies was confirmed in the blood sera of the mares inseminated with the semen of seropositive stallions in 2012 and 2013.
Keywords:
breeding stallions / semen / Equine arteritis virus / RT-PCR / seroprevalence / virus neutralizationSource:
Acta Veterinaria-Beograd, 2015, 65, 4, 557-567Publisher:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Funding / projects:
- Wild animal health monitoring and introduction of new biotechnology procedures in detection of infectious and zoonotic agents - risk analysis for human health, domestic and wild animal health and for environmental contamination (RS-MESTD-Technological Development (TD or TR)-31084)
- Ecological and viral investigation on the presents of emerging zoonoses in national parks in republic of Serbia (RS-MESTD-Technological Development (TD or TR)-37015)
DOI: 10.1515/acve-2015-0047
ISSN: 0567-8315
WoS: 000367467000011
Scopus: 2-s2.0-84955505773
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Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Lazić, Sava AU - Lupulović, Diana AU - Polaček, Vladimir AU - Valčić, Miroslav AU - Lazić, Gospava AU - Pasagić, Enisa AU - Petrović, Tamaš PY - 2015 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1180 AB - The results on serological testing of blood sera from stallions and mares used for breeding and the presence of the viral genome of Equine Arteritis Virus (EAV) in stallion semen are presented. The blood and semen samples were taken from a horse stable on the territory of the Republic of Serbia during 2012, 2013 and 2014. Detection of anti-EAV specific antibodies in blood sera was performed by the virus neutralization test (VNT), and identification of EAV genome RNA in stallion semen was done by reverse-transcriptase polymerase chain reaction (RT-PCR). In 2012, high seroprevalence of EAV was detected in the investigated stable. In total, 45% and 65 % of stallions and mares reacted positive, respectively, and the antibody titre values ranged between 2 and 10 log 2. High seroprevalence was confirmed in the same animals again in 2013. Out of two stallions tested semen samples in 2013, the viral genome was detected by RT-PCR in 3 examined semen samples from a seropositive stallion, while EAV was not detected in 3 semen samples of a seronegative stallion. During 2014, 11 semen samples were collected from two seropositive stallions. Again, the presence of EAV was confirmed by RT-PCR in all 8 semen samples originating from the same stallion with the EAV genome positive semen result in 2013, whereas the virus was not detected in semen samples originating from the second anti-EAV antibody positive stallion. The presence of EAV-specific antibodies was confirmed in the blood sera of the mares inseminated with the semen of seropositive stallions in 2012 and 2013. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Detection of equine arteritis virus in the semen of stallions in the republic of Serbia VL - 65 IS - 4 SP - 557 EP - 567 DO - 10.1515/acve-2015-0047 ER -
@article{ author = "Lazić, Sava and Lupulović, Diana and Polaček, Vladimir and Valčić, Miroslav and Lazić, Gospava and Pasagić, Enisa and Petrović, Tamaš", year = "2015", abstract = "The results on serological testing of blood sera from stallions and mares used for breeding and the presence of the viral genome of Equine Arteritis Virus (EAV) in stallion semen are presented. The blood and semen samples were taken from a horse stable on the territory of the Republic of Serbia during 2012, 2013 and 2014. Detection of anti-EAV specific antibodies in blood sera was performed by the virus neutralization test (VNT), and identification of EAV genome RNA in stallion semen was done by reverse-transcriptase polymerase chain reaction (RT-PCR). In 2012, high seroprevalence of EAV was detected in the investigated stable. In total, 45% and 65 % of stallions and mares reacted positive, respectively, and the antibody titre values ranged between 2 and 10 log 2. High seroprevalence was confirmed in the same animals again in 2013. Out of two stallions tested semen samples in 2013, the viral genome was detected by RT-PCR in 3 examined semen samples from a seropositive stallion, while EAV was not detected in 3 semen samples of a seronegative stallion. During 2014, 11 semen samples were collected from two seropositive stallions. Again, the presence of EAV was confirmed by RT-PCR in all 8 semen samples originating from the same stallion with the EAV genome positive semen result in 2013, whereas the virus was not detected in semen samples originating from the second anti-EAV antibody positive stallion. The presence of EAV-specific antibodies was confirmed in the blood sera of the mares inseminated with the semen of seropositive stallions in 2012 and 2013.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Detection of equine arteritis virus in the semen of stallions in the republic of Serbia", volume = "65", number = "4", pages = "557-567", doi = "10.1515/acve-2015-0047" }
Lazić, S., Lupulović, D., Polaček, V., Valčić, M., Lazić, G., Pasagić, E.,& Petrović, T.. (2015). Detection of equine arteritis virus in the semen of stallions in the republic of Serbia. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(4), 557-567. https://doi.org/10.1515/acve-2015-0047
Lazić S, Lupulović D, Polaček V, Valčić M, Lazić G, Pasagić E, Petrović T. Detection of equine arteritis virus in the semen of stallions in the republic of Serbia. in Acta Veterinaria-Beograd. 2015;65(4):557-567. doi:10.1515/acve-2015-0047 .
Lazić, Sava, Lupulović, Diana, Polaček, Vladimir, Valčić, Miroslav, Lazić, Gospava, Pasagić, Enisa, Petrović, Tamaš, "Detection of equine arteritis virus in the semen of stallions in the republic of Serbia" in Acta Veterinaria-Beograd, 65, no. 4 (2015):557-567, https://doi.org/10.1515/acve-2015-0047 . .