Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro
2015
Аутори
Maletić, JelenaĐelić, Ninoslav
Radaković, Milena
Maletić, Milan
Lakić, Nada
Kukolj, Vladimir
Aleksić, Nevenka
Anđelković, Marko
Stanimirović, Zoran
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Tulathromycin is a relatively new semi-synthetic macrolide antibiotic, a member of the triamilide group, approved primarly for the treatment of respiratory diseases in cattle and swine. Various genotoxicological studies indicated that tulathromycin is not genotoxic, but no available published data originate from the single-cell gel electrophoresis (Comet) assay. Therefore, the objective of this study was to examine whether it can induce primary DNA damage using in vitro Comet assay in isolated rat lymphocytes. Lymphocytes were treated with a broad spectrum of tulathromycin concentrations (from 1 to 100 mu M) and co-treatment with an antioxidant, catalase (100 IU/mL and 500 IU/mL) was performed. The highest concentrations of tulathromycin (50 and 100 mu M) caused significant increase of DNA damage in rat lymphocytes and catalase did not significantly reduce the DNA-damaging effect of tulathromycin. The results of this study indicate that tulathromycin induces genotoxic effects at high c...oncentrations, that catalase does not exert protective effect in this case.
Кључне речи:
comet assay / DNA damage / rat lymphocytes / tulathromycinИзвор:
Genetika, 2015, 47, 1, 339-348Издавач:
- Društvo genetičara Srbije, Beograd
Финансирање / пројекти:
- Молекуларно-генетичка и екофизиолошка истраживања у заштити аутохтоних анималних генетичких ресурса, очувању добробити, здравља и репродукције гајених животиња и производњи безбедне хране (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
DOI: 10.2298/GENSR1501339M
ISSN: 0534-0012
WoS: 000355248900030
Scopus: 2-s2.0-84929301003
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Maletić, Jelena AU - Đelić, Ninoslav AU - Radaković, Milena AU - Maletić, Milan AU - Lakić, Nada AU - Kukolj, Vladimir AU - Aleksić, Nevenka AU - Anđelković, Marko AU - Stanimirović, Zoran PY - 2015 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1183 AB - Tulathromycin is a relatively new semi-synthetic macrolide antibiotic, a member of the triamilide group, approved primarly for the treatment of respiratory diseases in cattle and swine. Various genotoxicological studies indicated that tulathromycin is not genotoxic, but no available published data originate from the single-cell gel electrophoresis (Comet) assay. Therefore, the objective of this study was to examine whether it can induce primary DNA damage using in vitro Comet assay in isolated rat lymphocytes. Lymphocytes were treated with a broad spectrum of tulathromycin concentrations (from 1 to 100 mu M) and co-treatment with an antioxidant, catalase (100 IU/mL and 500 IU/mL) was performed. The highest concentrations of tulathromycin (50 and 100 mu M) caused significant increase of DNA damage in rat lymphocytes and catalase did not significantly reduce the DNA-damaging effect of tulathromycin. The results of this study indicate that tulathromycin induces genotoxic effects at high concentrations, that catalase does not exert protective effect in this case. PB - Društvo genetičara Srbije, Beograd T2 - Genetika T1 - Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro VL - 47 IS - 1 SP - 339 EP - 348 DO - 10.2298/GENSR1501339M ER -
@article{ author = "Maletić, Jelena and Đelić, Ninoslav and Radaković, Milena and Maletić, Milan and Lakić, Nada and Kukolj, Vladimir and Aleksić, Nevenka and Anđelković, Marko and Stanimirović, Zoran", year = "2015", abstract = "Tulathromycin is a relatively new semi-synthetic macrolide antibiotic, a member of the triamilide group, approved primarly for the treatment of respiratory diseases in cattle and swine. Various genotoxicological studies indicated that tulathromycin is not genotoxic, but no available published data originate from the single-cell gel electrophoresis (Comet) assay. Therefore, the objective of this study was to examine whether it can induce primary DNA damage using in vitro Comet assay in isolated rat lymphocytes. Lymphocytes were treated with a broad spectrum of tulathromycin concentrations (from 1 to 100 mu M) and co-treatment with an antioxidant, catalase (100 IU/mL and 500 IU/mL) was performed. The highest concentrations of tulathromycin (50 and 100 mu M) caused significant increase of DNA damage in rat lymphocytes and catalase did not significantly reduce the DNA-damaging effect of tulathromycin. The results of this study indicate that tulathromycin induces genotoxic effects at high concentrations, that catalase does not exert protective effect in this case.", publisher = "Društvo genetičara Srbije, Beograd", journal = "Genetika", title = "Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro", volume = "47", number = "1", pages = "339-348", doi = "10.2298/GENSR1501339M" }
Maletić, J., Đelić, N., Radaković, M., Maletić, M., Lakić, N., Kukolj, V., Aleksić, N., Anđelković, M.,& Stanimirović, Z.. (2015). Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro. in Genetika Društvo genetičara Srbije, Beograd., 47(1), 339-348. https://doi.org/10.2298/GENSR1501339M
Maletić J, Đelić N, Radaković M, Maletić M, Lakić N, Kukolj V, Aleksić N, Anđelković M, Stanimirović Z. Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro. in Genetika. 2015;47(1):339-348. doi:10.2298/GENSR1501339M .
Maletić, Jelena, Đelić, Ninoslav, Radaković, Milena, Maletić, Milan, Lakić, Nada, Kukolj, Vladimir, Aleksić, Nevenka, Anđelković, Marko, Stanimirović, Zoran, "Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro" in Genetika, 47, no. 1 (2015):339-348, https://doi.org/10.2298/GENSR1501339M . .