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dc.creatorĐelić, Ninoslav
dc.creatorRadaković, Milena
dc.creatorSpremo-Potparević, Biljana
dc.creatorŽivković, Lada
dc.creatorBajić, Vladan
dc.creatorStevanović, Jevrosima
dc.creatorStanimirović, Zoran
dc.date.accessioned2020-06-03T13:55:07Z
dc.date.available2020-06-03T13:55:07Z
dc.date.issued2015
dc.identifier.issn0887-2333
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1197
dc.description.abstractCatechol groups can be involved in redox cycling accompanied by generation of reactive oxygen species (ROS) which may lead to oxidative damage of cellular macromolecules including DNA. The objective of this investigation was to evaluate possible genotoxic effects of a natural catecholamine adrenaline in cultured human lymphocytes using cytogenetic (sister chromatid exchange and micronuclei) and the single cell gel electrophoresis (Comet) assay. In cytogenetic tests, six experimental concentrations of adrenaline were used in a range from 0.01-500 mu M. There were no indications of genotoxic effects of adrenaline in sister chromatid exchange and micronucleus tests. However, at four highest concentrations of adrenaline (5 mu M, 50 mu M, 150 mu M and 300 mu M) we observed a decreased mitotic index and cell-cycle delay. In addition, in the Comet assay we used adrenaline in a range from 0.0005-500 mu M, at two treatment times: 15 min or 60 min. In contrast to cytogenetic analysis, there was a dose-dependent increase of DNA damage detected in the Comet assay. These effects were significantly reduced by concomitant treatment with quercetin or catalase. Therefore, the obtained results indicate that adrenaline may exhibit genotoxic effects in cultured human lymphocytes, most likely due to production of reactive oxygen species.en
dc.publisherPergamon-Elsevier Science Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46002/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173034/RS//
dc.rightsrestrictedAccess
dc.sourceToxicology in Vitro
dc.subjectSister chromatid exchangeen
dc.subjectMicronucleien
dc.subjectDNA damageen
dc.subjectOxidative stressen
dc.titleEvaluation of cytogenetic and DNA damage in human lymphocytes treated with adrenaline in vitroen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractБајић, Владан; Стевановић, Јевросима; Живковић, Лада; Ђелић, Нинослав; Спремо-Потпаревић, Биљана; Радаковић, Милена; Станимировић, Зоран;
dc.citation.volume29
dc.citation.issue1
dc.citation.spage27
dc.citation.epage33
dc.citation.other29(1): 27-33
dc.citation.rankM21
dc.identifier.wos000347746600004
dc.identifier.doi10.1016/j.tiv.2014.08.001
dc.identifier.pmid25172298
dc.identifier.scopus2-s2.0-84907661875
dc.identifier.rcubconv_2036
dc.type.versionpublishedVersion


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