Morphological features and kit receptor expression in canine cutaneous mast cell tumor and systemic mastocytosis
2015
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Authors
Marinković, DarkoMilčić-Matić, Natalija
Jovanović, Milan
Vučićević, Ivana
Nešić, Slađan
Aničić, Milan
Aleksić-Kovačević, Sanja
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Mast cell neoplasia in dogs can occur in two different forms: common as cutaneous tumor, or less common as a systemic form of neoplastic mast cell proliferation - systemic mastocytosis. The aim of this study was to compare the histological and cytological features, KIT receptor expression and presence of c-KIT proto-oncogene mutations in neoplastic cells of dogs with canine cutaneous mast cell tumor (CMCT) and systemic mastocytosis. Microscopical examination of the cytological smears obtained from all selected dogs revealed that cellular specimens were constituted mostly of round cells with a central nuclei and fine to coarse purple cytoplasmic granules. Histopathological examination of skin samples of dogs with CMCT and a dog with systemic mastocytosis showed proliferation of the neoplastic mast cells in the superficial and/or deep dermis. Similar findings were observed in tissue samples derived from lymph nodes, spleen, liver, myocardium and kidneys of a dog with systemic mastocytosi...s. Three dogs with high grade CMCT as well as one dog with systemic mastocytosis showed cytoplasmic CD117 expression, while 3 dogs with low grade CMCT, had membranous expression of CD117. Based on our study, histological features and cytoplasmic CD117 expression in neoplastic cells of dogs with systemic mastocytosis are similar to those in dogs with high grade CMCTs. Nevertheless, mutations of c-KIT proto-oncogene were not found in tumor samples either from dogs with CMCT or dog with systemic mastocytosis.
Keywords:
dog / systemic mastocytosis / cutaneous mast cell tumor / KIT receptor expression / histopathology / cytologySource:
Acta Veterinaria-Beograd, 2015, 65, 2, 226-237Publisher:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Funding / projects:
- Development of herbal medicines and biocides on the basis of Carvacrol, Thymol and Cinnamaldehyde for use in veterinary medicine, animal husbandry and food production without harmful residues (RS-MESTD-Technological Development (TD or TR)-31087)
- Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
DOI: 10.1515/acve-2015-0019
ISSN: 0567-8315
WoS: 000356832100007
Scopus: 2-s2.0-84933518837
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Fakultet veterinarske medicineTY - JOUR AU - Marinković, Darko AU - Milčić-Matić, Natalija AU - Jovanović, Milan AU - Vučićević, Ivana AU - Nešić, Slađan AU - Aničić, Milan AU - Aleksić-Kovačević, Sanja PY - 2015 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1199 AB - Mast cell neoplasia in dogs can occur in two different forms: common as cutaneous tumor, or less common as a systemic form of neoplastic mast cell proliferation - systemic mastocytosis. The aim of this study was to compare the histological and cytological features, KIT receptor expression and presence of c-KIT proto-oncogene mutations in neoplastic cells of dogs with canine cutaneous mast cell tumor (CMCT) and systemic mastocytosis. Microscopical examination of the cytological smears obtained from all selected dogs revealed that cellular specimens were constituted mostly of round cells with a central nuclei and fine to coarse purple cytoplasmic granules. Histopathological examination of skin samples of dogs with CMCT and a dog with systemic mastocytosis showed proliferation of the neoplastic mast cells in the superficial and/or deep dermis. Similar findings were observed in tissue samples derived from lymph nodes, spleen, liver, myocardium and kidneys of a dog with systemic mastocytosis. Three dogs with high grade CMCT as well as one dog with systemic mastocytosis showed cytoplasmic CD117 expression, while 3 dogs with low grade CMCT, had membranous expression of CD117. Based on our study, histological features and cytoplasmic CD117 expression in neoplastic cells of dogs with systemic mastocytosis are similar to those in dogs with high grade CMCTs. Nevertheless, mutations of c-KIT proto-oncogene were not found in tumor samples either from dogs with CMCT or dog with systemic mastocytosis. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Morphological features and kit receptor expression in canine cutaneous mast cell tumor and systemic mastocytosis VL - 65 IS - 2 SP - 226 EP - 237 DO - 10.1515/acve-2015-0019 ER -
@article{ author = "Marinković, Darko and Milčić-Matić, Natalija and Jovanović, Milan and Vučićević, Ivana and Nešić, Slađan and Aničić, Milan and Aleksić-Kovačević, Sanja", year = "2015", abstract = "Mast cell neoplasia in dogs can occur in two different forms: common as cutaneous tumor, or less common as a systemic form of neoplastic mast cell proliferation - systemic mastocytosis. The aim of this study was to compare the histological and cytological features, KIT receptor expression and presence of c-KIT proto-oncogene mutations in neoplastic cells of dogs with canine cutaneous mast cell tumor (CMCT) and systemic mastocytosis. Microscopical examination of the cytological smears obtained from all selected dogs revealed that cellular specimens were constituted mostly of round cells with a central nuclei and fine to coarse purple cytoplasmic granules. Histopathological examination of skin samples of dogs with CMCT and a dog with systemic mastocytosis showed proliferation of the neoplastic mast cells in the superficial and/or deep dermis. Similar findings were observed in tissue samples derived from lymph nodes, spleen, liver, myocardium and kidneys of a dog with systemic mastocytosis. Three dogs with high grade CMCT as well as one dog with systemic mastocytosis showed cytoplasmic CD117 expression, while 3 dogs with low grade CMCT, had membranous expression of CD117. Based on our study, histological features and cytoplasmic CD117 expression in neoplastic cells of dogs with systemic mastocytosis are similar to those in dogs with high grade CMCTs. Nevertheless, mutations of c-KIT proto-oncogene were not found in tumor samples either from dogs with CMCT or dog with systemic mastocytosis.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Morphological features and kit receptor expression in canine cutaneous mast cell tumor and systemic mastocytosis", volume = "65", number = "2", pages = "226-237", doi = "10.1515/acve-2015-0019" }
Marinković, D., Milčić-Matić, N., Jovanović, M., Vučićević, I., Nešić, S., Aničić, M.,& Aleksić-Kovačević, S.. (2015). Morphological features and kit receptor expression in canine cutaneous mast cell tumor and systemic mastocytosis. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(2), 226-237. https://doi.org/10.1515/acve-2015-0019
Marinković D, Milčić-Matić N, Jovanović M, Vučićević I, Nešić S, Aničić M, Aleksić-Kovačević S. Morphological features and kit receptor expression in canine cutaneous mast cell tumor and systemic mastocytosis. in Acta Veterinaria-Beograd. 2015;65(2):226-237. doi:10.1515/acve-2015-0019 .
Marinković, Darko, Milčić-Matić, Natalija, Jovanović, Milan, Vučićević, Ivana, Nešić, Slađan, Aničić, Milan, Aleksić-Kovačević, Sanja, "Morphological features and kit receptor expression in canine cutaneous mast cell tumor and systemic mastocytosis" in Acta Veterinaria-Beograd, 65, no. 2 (2015):226-237, https://doi.org/10.1515/acve-2015-0019 . .