Effects of estrogen and luteinizing-releasing hormone on chromatin in CA3 region of male rat hippocampus

Abstract

The structure of heterochromatin protects genome from undesirable recombinations, while estrogen and luteinizing-releasing hormone (LHRH) cause changes in structure of chromatin. The objective of this study was to prove the connection of neonatally applied estro­gen and LHRH, with shown effects on chromatin in hippocampus of adult male rats. Stereological results have shown that total surface of neuron area in estrogen treated animals significantly reduced in regard to the control group as well as groups treated with LHRH. The number of heterochromatin granules in either estrogen or LHRH treated groups significantly increased, while in the last fourth group treated with both estrogen and LHRH the difference is even more expressed, both in regard to the control group and oth­er groups.

Struktura heterohromatina štiti genom od neželjenih rekombinacija, dok estrogen i luteinizirajući-oslobađajući hormon (LHRH) uzrokuju promene u samoj strukturi hromatina. Studija je napravljena sa ciljem dokazivanja povezanosti neonatalno aplikovanog estrogena i LHRH, sa ispoljenim efektima na hromatinu u hipokampusu odraslih mužjaka pacova. Stereološki rezultati su pokazali da je ukupna površina neurona kod životinja tretiranih estrogenom značajno smanjena u odnosu na kontrolnu i grupe tretirane LHRH. Broj granula heterohromatina u grupama tretiranim estrogenom i LHRH je primetno povećan, dok je u poslednjoj, četvrtoj grupi tretiranoj i estrogenom i LHRH ova razlika još izraženija, kako u odnosu na kontrolnu, tako i u odnosu na ostale grupe.