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Ispitivanje nekih bioloških karakteristika glikoproteinskih subjedinica soja PHY-LMV.42 virusa Newcastle bolesti živine

dc.creatorMilić, Nenad
dc.creatorNišavić, Jakov
dc.creatorBorozan, Sunčica
dc.creatorZorić, Andrea
dc.creatorLazić, Sava
dc.creatorPetrović, Tamaš
dc.creatorRašić, Zoran
dc.date.accessioned2020-06-03T13:56:46Z
dc.date.available2020-06-03T13:56:46Z
dc.date.issued2015
dc.identifier.issn0350-2457
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1221
dc.description.abstractThe objective of our work was to investigate some biological characteristics of purified glycoprotein subunits of Newcastle disease virus strain PHY-LMV.42 isolated from pigeons for the purpose of vaccine production. PHY-LMV.42 strain of Newcastle disease virus was multiplied by successive passages in embryonated eggs and identified by the methods of Reverse transcriptase PCR and Real-Time PCR along with F gene sequencing. Proving the presence of HN and F antigene in the virus subunits samples was carried out by hemagglutination inhibition method with referent immune sera. Biochemical characterization of glycoprotein subunits was performed by SDS-PAGE method as well as liquid chromatography with mass spectrometry (LC ESI-TOF-MS/MS). Testing for the virus subunits immunogenicity was carried out in biological experiment on 75 laying hens Tetra-SSL and 25 chickens Isa Brown by inducing an artificial infection with Hertz 33 strain of the virus. Low concentrations of the virus antigens of 0.36 mg/ml along with glycoprotein fractions of 77 i 58 kDa manifested a strong hemagglutination activity of 4096 HJ/0,1ml. The subunit vaccines of 256 and 128 HJ/0.5 ml induced a protective immune response in all the vaccinated animals. Based on the obtained results it can be concluded that low concentrations of purified virus subunits of PHY-LMV.42 strain can be used for preparing of effective vaccines.en
dc.description.abstractCilj našeg istraživanja je bilo ispitivanje bioloških karakteristika prečišćenih glikoproteinskih subjedinica soja PHY-LMV.42 virusa Newcastle bolesti izolovanog iz golubova radi njihovog korišćenja za pripremanje vakcine. Soj PHY-LMV.42 virusa Newcastle bolesti je umnožavan sukcesivnim pasažama u kokošijim embrionima i identifikovan metodama Reverse transcriptase PCR i Real- Time PCR uz sekvenciranje F gena. Dokazivanje prisustva HN i F antigena u uzorcima virusnih subjedinica vršeno je metodom inhibicije hemaglutinacije sa referentnim imunim serumima. Biohemijska karakterizacija glikoproteinskih subjedinica izvršena je primenom metoda SDS-PAGE i tečne hromatografije sa masenom spektrometrijom (LC ESI-TOF-MS/MS). Ispitivanje imunogenosti virusnih subjedinica sprovedeno je u biološkom ogledu na ukupno 75 kokoši nosilja Tetra-SSL i 25 pilića Isa Brown uz izvođenje veštačke infekcije sojem Hertz 33 navedenog virusa. Niske koncentracije virusnih antigena od 0,36 mg/ml sa glikoproteinskim frakcijama od 77 i 58 kDa su ispoljavale snažnu hemaglutinacionu aktivnost od 4096 HJ/0,1ml. Subjedinične vakcine od 256 i 128 HJ/0,5 ml, indukovale su imunološki odgovor zaštitnog karaktera kod svih vakcinisanih životinja. Na osnovu dobijenih rezultata može se zaključiti da se niske koncentracije prečišćenih virusnih subjedinica soja PHY-LMV.42 mogu koristiti za pripremanje efikasne vakcine. PR Projekat Ministarstva nauke Republike Srbije, br. TR 31008.sr
dc.publisherUniverzitet u Beogradu - Fakultet veterinarske medicine, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31008/RS//
dc.rightsopenAccess
dc.sourceVeterinarski glasnik
dc.subjectNewcastle disease virusen
dc.subjectglycoprotein subunitsen
dc.subjectSDS-PAGEen
dc.subjectLC ESI-TOF-MS/MSen
dc.subjectimmunizationen
dc.subjectvirus Newcastle bolestisr
dc.subjectglikoproteinske subjedinicesr
dc.subjectSDS-PAGEsr
dc.subjectLC ESI-TOF-MS/MSsr
dc.subjectimunizacijasr
dc.titleExamination of some biological properties of glycoprotein subunits of PHY-LMV.42 strain of Newcastle disease virusen
dc.titleIspitivanje nekih bioloških karakteristika glikoproteinskih subjedinica soja PHY-LMV.42 virusa Newcastle bolesti živinesr
dc.typearticle
dc.rights.licenseBY
dcterms.abstractЗорић, Aндреа; Борозан, Сунчица; Лазић, Сава; Рашић, Зоран; Петровић, Тамаш; Милић, Ненад; Нишавић, Јаков;
dc.citation.volume69
dc.citation.issue5-6
dc.citation.spage337
dc.citation.epage355
dc.citation.other69(5-6): 337-355
dc.citation.rankM51
dc.identifier.doi10.2298/VETGL1506337M
dc.identifier.fulltexthttp://veterinar.vet.bg.ac.rs/bitstream/id/200/1220.pdf
dc.identifier.rcubconv_362
dc.type.versionpublishedVersion


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