Comparison of two analytical methods (ELISA and LC-MS/MS) for determination of aflatoxin B-1 in corn and aflatoxin M-1 in milk
2015
Authors
Stefanović, SrđanSpirić, Danka
Petronijević, Radivoj
Nedeljković-Trailović, Jelena
Milićević, Dragan
Nikolić, Dragica M.
Janković, Saša
Conference object (Published version)
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The aim of this paper is to assess the closeness of agreement between results of ELISA and LC-MS/MS methods for determination of aflatoxin B-1 in corn and aflatoxin M-1 in milk. Samples of corn (n=100) and milk (n=250) were simultaneously analyzed using ELISA and LC-MS/MS methods, after the severe drought that affected Serbia in summer 2012 resulting in occurrence of aflatoxin B1 in corn and aflatoxin M-1 in milk. Regression analysis showed higher level of agreement between aflatoxin B-1 samples (R2=0.994), compared to aflatoxin M-1 samples (r(2)=0.920). However, both techniques were satisfactory in meeting the requirements for official control purposes.
Keywords:
aflatoxin B1 / aflatoxin M1 / corn / milk / ELISA / LC-MS/MSSource:
58th International Meat Industry Conference (MEATCON2015), 2015, 5, 270-273Publisher:
- Elsevier Science Bv, Amsterdam
Funding / projects:
- Improvement and development of hygienic and technological procedures in production of animal originating foodstuffs with the aim of producing high-quality and safe products competetive on the global market (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46009)
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Fakultet veterinarske medicineTY - CONF AU - Stefanović, Srđan AU - Spirić, Danka AU - Petronijević, Radivoj AU - Nedeljković-Trailović, Jelena AU - Milićević, Dragan AU - Nikolić, Dragica M. AU - Janković, Saša PY - 2015 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1252 AB - The aim of this paper is to assess the closeness of agreement between results of ELISA and LC-MS/MS methods for determination of aflatoxin B-1 in corn and aflatoxin M-1 in milk. Samples of corn (n=100) and milk (n=250) were simultaneously analyzed using ELISA and LC-MS/MS methods, after the severe drought that affected Serbia in summer 2012 resulting in occurrence of aflatoxin B1 in corn and aflatoxin M-1 in milk. Regression analysis showed higher level of agreement between aflatoxin B-1 samples (R2=0.994), compared to aflatoxin M-1 samples (r(2)=0.920). However, both techniques were satisfactory in meeting the requirements for official control purposes. PB - Elsevier Science Bv, Amsterdam C3 - 58th International Meat Industry Conference (MEATCON2015) T1 - Comparison of two analytical methods (ELISA and LC-MS/MS) for determination of aflatoxin B-1 in corn and aflatoxin M-1 in milk VL - 5 SP - 270 EP - 273 DO - 10.1016/j.profoo.2015.09.077 ER -
@conference{ author = "Stefanović, Srđan and Spirić, Danka and Petronijević, Radivoj and Nedeljković-Trailović, Jelena and Milićević, Dragan and Nikolić, Dragica M. and Janković, Saša", year = "2015", abstract = "The aim of this paper is to assess the closeness of agreement between results of ELISA and LC-MS/MS methods for determination of aflatoxin B-1 in corn and aflatoxin M-1 in milk. Samples of corn (n=100) and milk (n=250) were simultaneously analyzed using ELISA and LC-MS/MS methods, after the severe drought that affected Serbia in summer 2012 resulting in occurrence of aflatoxin B1 in corn and aflatoxin M-1 in milk. Regression analysis showed higher level of agreement between aflatoxin B-1 samples (R2=0.994), compared to aflatoxin M-1 samples (r(2)=0.920). However, both techniques were satisfactory in meeting the requirements for official control purposes.", publisher = "Elsevier Science Bv, Amsterdam", journal = "58th International Meat Industry Conference (MEATCON2015)", title = "Comparison of two analytical methods (ELISA and LC-MS/MS) for determination of aflatoxin B-1 in corn and aflatoxin M-1 in milk", volume = "5", pages = "270-273", doi = "10.1016/j.profoo.2015.09.077" }
Stefanović, S., Spirić, D., Petronijević, R., Nedeljković-Trailović, J., Milićević, D., Nikolić, D. M.,& Janković, S.. (2015). Comparison of two analytical methods (ELISA and LC-MS/MS) for determination of aflatoxin B-1 in corn and aflatoxin M-1 in milk. in 58th International Meat Industry Conference (MEATCON2015) Elsevier Science Bv, Amsterdam., 5, 270-273. https://doi.org/10.1016/j.profoo.2015.09.077
Stefanović S, Spirić D, Petronijević R, Nedeljković-Trailović J, Milićević D, Nikolić DM, Janković S. Comparison of two analytical methods (ELISA and LC-MS/MS) for determination of aflatoxin B-1 in corn and aflatoxin M-1 in milk. in 58th International Meat Industry Conference (MEATCON2015). 2015;5:270-273. doi:10.1016/j.profoo.2015.09.077 .
Stefanović, Srđan, Spirić, Danka, Petronijević, Radivoj, Nedeljković-Trailović, Jelena, Milićević, Dragan, Nikolić, Dragica M., Janković, Saša, "Comparison of two analytical methods (ELISA and LC-MS/MS) for determination of aflatoxin B-1 in corn and aflatoxin M-1 in milk" in 58th International Meat Industry Conference (MEATCON2015), 5 (2015):270-273, https://doi.org/10.1016/j.profoo.2015.09.077 . .