The influence of bovine seminal plasma on BHV -1, EHV-1, BVD and Morbus Aujeszky virus replication in vitro

Abstract

The aim of this investigation was to estimate the possible influence of bovine seminal plasma (BSP) on the replication in vitro of some sexually transmitted viruses: BHV - 1 (bovine herpes virus - 1) and BVD (bovine viral diarrhea) significant for bovine reproduction. In addition, we tested the influence of the on EHV-1 (equine herpes virus - 1) and Morbus Aujeszcky virus replication in the same model. Our results showed that BSP has an inhibitory effect on the replication in vitro of all tested viruses and their strains. This activity was lost following dialysis suggesting that the active inhibitory factor is of small molecular veight (less than 12 kDa).Furthermore, inhibition was preserved after repeated freezing and thawing and after inactivation for 30 min at 56 °C indicating that the inhibitory factor is heat stable.