The investigation of the presence of Clostridium botulinum spores in honey in Serbia
2015
Authors
Matović, KazimirBaltić, Milan Ž.
Nedić, Nebojša
Dmitrić, Marko
Nenadić, Dragan
Vasković, Nikola
Jevtić, Goran
Mišić, Dušan
Conference object (Published version)
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The presence of Clostridium botulinum spores in 59 honey samples originating from different regions of the Republic of Serbia was studied. In addition to microbiological methods, after enrichment, centrifugation and membrane filtration, molecular methods (PCR methods) were utilized. The number of spores in PCR positive samples was estimated by the most probable number (MPN) method. PCR confirmed C. botulinum spores in 5 (8.47%) honey samples. MPN of spores varied from 20/kg to 204/kg honey. PCR was more sensitive than cultural methods. Natural honey contamination with C. botulinum spores is low-level and not homogeneous, and therefore, PCR methods require multiple sub-sampling.
Keywords:
Honey / Clostridium botulinum / microbiological methods / PCR / MPNSource:
58th International Meat Industry Conference (MEATCON2015), 2015, 5, 180-183Publisher:
- Elsevier Science Bv, Amsterdam
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Institution/Community
Fakultet veterinarske medicineTY - CONF AU - Matović, Kazimir AU - Baltić, Milan Ž. AU - Nedić, Nebojša AU - Dmitrić, Marko AU - Nenadić, Dragan AU - Vasković, Nikola AU - Jevtić, Goran AU - Mišić, Dušan PY - 2015 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1267 AB - The presence of Clostridium botulinum spores in 59 honey samples originating from different regions of the Republic of Serbia was studied. In addition to microbiological methods, after enrichment, centrifugation and membrane filtration, molecular methods (PCR methods) were utilized. The number of spores in PCR positive samples was estimated by the most probable number (MPN) method. PCR confirmed C. botulinum spores in 5 (8.47%) honey samples. MPN of spores varied from 20/kg to 204/kg honey. PCR was more sensitive than cultural methods. Natural honey contamination with C. botulinum spores is low-level and not homogeneous, and therefore, PCR methods require multiple sub-sampling. PB - Elsevier Science Bv, Amsterdam C3 - 58th International Meat Industry Conference (MEATCON2015) T1 - The investigation of the presence of Clostridium botulinum spores in honey in Serbia VL - 5 SP - 180 EP - 183 DO - 10.1016/j.profoo.2015.09.051 ER -
@conference{ author = "Matović, Kazimir and Baltić, Milan Ž. and Nedić, Nebojša and Dmitrić, Marko and Nenadić, Dragan and Vasković, Nikola and Jevtić, Goran and Mišić, Dušan", year = "2015", abstract = "The presence of Clostridium botulinum spores in 59 honey samples originating from different regions of the Republic of Serbia was studied. In addition to microbiological methods, after enrichment, centrifugation and membrane filtration, molecular methods (PCR methods) were utilized. The number of spores in PCR positive samples was estimated by the most probable number (MPN) method. PCR confirmed C. botulinum spores in 5 (8.47%) honey samples. MPN of spores varied from 20/kg to 204/kg honey. PCR was more sensitive than cultural methods. Natural honey contamination with C. botulinum spores is low-level and not homogeneous, and therefore, PCR methods require multiple sub-sampling.", publisher = "Elsevier Science Bv, Amsterdam", journal = "58th International Meat Industry Conference (MEATCON2015)", title = "The investigation of the presence of Clostridium botulinum spores in honey in Serbia", volume = "5", pages = "180-183", doi = "10.1016/j.profoo.2015.09.051" }
Matović, K., Baltić, M. Ž., Nedić, N., Dmitrić, M., Nenadić, D., Vasković, N., Jevtić, G.,& Mišić, D.. (2015). The investigation of the presence of Clostridium botulinum spores in honey in Serbia. in 58th International Meat Industry Conference (MEATCON2015) Elsevier Science Bv, Amsterdam., 5, 180-183. https://doi.org/10.1016/j.profoo.2015.09.051
Matović K, Baltić MŽ, Nedić N, Dmitrić M, Nenadić D, Vasković N, Jevtić G, Mišić D. The investigation of the presence of Clostridium botulinum spores in honey in Serbia. in 58th International Meat Industry Conference (MEATCON2015). 2015;5:180-183. doi:10.1016/j.profoo.2015.09.051 .
Matović, Kazimir, Baltić, Milan Ž., Nedić, Nebojša, Dmitrić, Marko, Nenadić, Dragan, Vasković, Nikola, Jevtić, Goran, Mišić, Dušan, "The investigation of the presence of Clostridium botulinum spores in honey in Serbia" in 58th International Meat Industry Conference (MEATCON2015), 5 (2015):180-183, https://doi.org/10.1016/j.profoo.2015.09.051 . .