The investigation of the presence of Clostridium botulinum spores in honey in Serbia
Baltić, Milan Ž.
Conference object (Published version)
MetadataShow full item record
The presence of Clostridium botulinum spores in 59 honey samples originating from different regions of the Republic of Serbia was studied. In addition to microbiological methods, after enrichment, centrifugation and membrane filtration, molecular methods (PCR methods) were utilized. The number of spores in PCR positive samples was estimated by the most probable number (MPN) method. PCR confirmed C. botulinum spores in 5 (8.47%) honey samples. MPN of spores varied from 20/kg to 204/kg honey. PCR was more sensitive than cultural methods. Natural honey contamination with C. botulinum spores is low-level and not homogeneous, and therefore, PCR methods require multiple sub-sampling.
Keywords:Honey / Clostridium botulinum / microbiological methods / PCR / MPN
Source:58th International Meat Industry Conference (MEATCON2015), 2015, 5, 180-183
- Elsevier Science Bv, Amsterdam