Приказ основних података о документу
Interaction of carvacrol with the Ascaris suum nicotinic acetylcholine receptors and gamma-aminobutyric acid receptors, potential mechanism of antinematodal action
dc.creator | Trailović, Saša | |
dc.creator | Marjanović, Đorđe | |
dc.creator | Nedeljković-Trailović, Jelena | |
dc.creator | Robertson, Alan P. | |
dc.creator | Martin, Richard J. | |
dc.date.accessioned | 2020-06-03T14:01:40Z | |
dc.date.available | 2020-06-03T14:01:40Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 0932-0113 | |
dc.identifier.uri | https://vet-erinar.vet.bg.ac.rs/handle/123456789/1290 | |
dc.description.abstract | Essential plant oils (or their active principles) are safe to use and a potentially attractive alternative to current antiparasitic drugs. In the present study, we tested the effects of carvacrol on the isolated tissues of Ascaris suum and investigated potential interactions with other antiparasitic drugs. We used somatic muscle flaps for contraction assays, as well as for electrophysiological investigations. Carvacrol 300 mu M highly significantly inhibited contractions caused by 1, 3, 10, 30, and 100 mu M of ACh (p = 0.0023, p = 0.0002, p = 0.0002, p < 0.0001, and p < 0.0001). The control EC50 for acetylcholine was 8.87 mu M (log EC50 = 0.95 +/- 0.26), while R (max) was 2.53 +/- 0.24 g. The EC50 of acetylcholine in the presence of 300 mu M of carvacrol was 27.71 mu M (log EC50 = 1.44 +/- 0.28) and the R (max) decreased to 1.63 +/- 0.32 g. Furthermore, carvacrol highly significant potentiates inhibitory effect of GABA and piperazine on the contractions induced by ACh. However, carvacrol (100 and 300 mu M), did not produce any changes in the membrane potential or conductance of the A. suum muscle cell. While, 300 mu M of carvacrol showed a significant inhibitory effect on ACh-induced depolarization response. The mean control depolarization was 13.58 +/- 0.66 mV and decreased in presence of carvacrol to 4.50 +/- 1.02 mV (p < 0.0001). Mean control Delta g was 0.168 +/- 0.017 mu S, while in the presence of 300 mu M of carvacrol, Delta g significantly decreased to 0.060 +/- 0.018 Delta S (p = 0.0017). The inhibitory effect on contractions may be the explanation of the antinematodal potential of carvacrol. Moreover, inhibition of depolarizations caused by ACh and reduction of conductance changes directly points to an interaction with the nAChR in A. suum. | en |
dc.publisher | Springer, New York | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31087/RS// | |
dc.rights | openAccess | |
dc.source | Parasitology Research | |
dc.subject | A. suum | en |
dc.subject | Carvacrol | en |
dc.subject | GABA | en |
dc.title | Interaction of carvacrol with the Ascaris suum nicotinic acetylcholine receptors and gamma-aminobutyric acid receptors, potential mechanism of antinematodal action | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Мартин, Рицхард Ј.; Робертсон, Aлан П.; Марјановић, Ђорђе; Траиловић, Саша; Недељковић-Траиловић, Јелена; | |
dc.citation.volume | 114 | |
dc.citation.issue | 8 | |
dc.citation.spage | 3059 | |
dc.citation.epage | 3068 | |
dc.citation.other | 114(8): 3059-3068 | |
dc.citation.rank | M22 | |
dc.identifier.wos | 000358410600028 | |
dc.identifier.doi | 10.1007/s00436-015-4508-x | |
dc.identifier.pmid | 25944741 | |
dc.identifier.scopus | 2-s2.0-84938956124 | |
dc.identifier.fulltext | https://vet-erinar.vet.bg.ac.rs/bitstream/id/259/1289.pdf | |
dc.type.version | publishedVersion |