Clinical babesiosis and molecular identification of babesia canis and babesia gibsoni infections in dogs from Serbia
2015
Аутори
Davitkov, DarkoVučićević, Miloš
Stevanović, Jevrosima
Krstić, Vanja
Tomanović, Snežana
Glavinić, Uroš
Stanimirović, Zoran
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Canine babesiosis is a frequent and clinically significant tick-borne disease. Sixty symptomatic dogs with clinical findings compatible with babesiosis were included in this study conducted in Serbia. After clinical examination, blood samples were taken for microscopic examination, complete blood count (CBC), Canine SNAP 4Dx Test, DNA analyses and sequencing. The main clinical signs included apathy, anorexia, fever, brown/red discoloration of urine, pale mucous membranes, icterus, splenomegaly, and vomiting. The main clinicopathological findings in Babesia infections were a slight to severe thrombocytopenia and a mild to very severe normocytic normochromic anaemia. Microscopic evaluation revealed 58 positive samples with the presence of large and small intraerythrocytic piroplasms in 57 and 1 sample(s), respectively. No co-infections were found using SNAP test. Two Babesia species, B. canis (58/60) and B. gibsoni (2/60), were differentiated by polymerase chain reaction-restriction frag...ment length polymorphism (PCR-RFLP). Species identification was further confirmed by sequencing PCR products of B. gibsoni samples and six randomly selected B. canis samples. All dogs were treated with imidocarb dipropionate (6.6 mg/kg of body weight), given intramuscularly twice at an interval of 14 days. This report presents the first molecular evidence of the occurrence of B. gibsoni and B. canis, confirmed by DNA sequencing, in sick dogs from Serbia.
Кључне речи:
Babesia canis/gibsoni / dogs / clinical signs / PCR-RFLP / sequencing / SerbiaИзвор:
Acta Veterinaria Hungarica, 2015, 63, 2, 199-208Издавач:
- Akademiai Kiado Zrt, Budapest
Финансирање / пројекти:
- Молекуларно-генетичка и екофизиолошка истраживања у заштити аутохтоних анималних генетичких ресурса, очувању добробити, здравља и репродукције гајених животиња и производњи безбедне хране (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
DOI: 10.1556/AVet.2015.017
ISSN: 0236-6290
PubMed: 26051258
WoS: 000357079000006
Scopus: 2-s2.0-84946127446
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Davitkov, Darko AU - Vučićević, Miloš AU - Stevanović, Jevrosima AU - Krstić, Vanja AU - Tomanović, Snežana AU - Glavinić, Uroš AU - Stanimirović, Zoran PY - 2015 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1293 AB - Canine babesiosis is a frequent and clinically significant tick-borne disease. Sixty symptomatic dogs with clinical findings compatible with babesiosis were included in this study conducted in Serbia. After clinical examination, blood samples were taken for microscopic examination, complete blood count (CBC), Canine SNAP 4Dx Test, DNA analyses and sequencing. The main clinical signs included apathy, anorexia, fever, brown/red discoloration of urine, pale mucous membranes, icterus, splenomegaly, and vomiting. The main clinicopathological findings in Babesia infections were a slight to severe thrombocytopenia and a mild to very severe normocytic normochromic anaemia. Microscopic evaluation revealed 58 positive samples with the presence of large and small intraerythrocytic piroplasms in 57 and 1 sample(s), respectively. No co-infections were found using SNAP test. Two Babesia species, B. canis (58/60) and B. gibsoni (2/60), were differentiated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Species identification was further confirmed by sequencing PCR products of B. gibsoni samples and six randomly selected B. canis samples. All dogs were treated with imidocarb dipropionate (6.6 mg/kg of body weight), given intramuscularly twice at an interval of 14 days. This report presents the first molecular evidence of the occurrence of B. gibsoni and B. canis, confirmed by DNA sequencing, in sick dogs from Serbia. PB - Akademiai Kiado Zrt, Budapest T2 - Acta Veterinaria Hungarica T1 - Clinical babesiosis and molecular identification of babesia canis and babesia gibsoni infections in dogs from Serbia VL - 63 IS - 2 SP - 199 EP - 208 DO - 10.1556/AVet.2015.017 ER -
@article{ author = "Davitkov, Darko and Vučićević, Miloš and Stevanović, Jevrosima and Krstić, Vanja and Tomanović, Snežana and Glavinić, Uroš and Stanimirović, Zoran", year = "2015", abstract = "Canine babesiosis is a frequent and clinically significant tick-borne disease. Sixty symptomatic dogs with clinical findings compatible with babesiosis were included in this study conducted in Serbia. After clinical examination, blood samples were taken for microscopic examination, complete blood count (CBC), Canine SNAP 4Dx Test, DNA analyses and sequencing. The main clinical signs included apathy, anorexia, fever, brown/red discoloration of urine, pale mucous membranes, icterus, splenomegaly, and vomiting. The main clinicopathological findings in Babesia infections were a slight to severe thrombocytopenia and a mild to very severe normocytic normochromic anaemia. Microscopic evaluation revealed 58 positive samples with the presence of large and small intraerythrocytic piroplasms in 57 and 1 sample(s), respectively. No co-infections were found using SNAP test. Two Babesia species, B. canis (58/60) and B. gibsoni (2/60), were differentiated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Species identification was further confirmed by sequencing PCR products of B. gibsoni samples and six randomly selected B. canis samples. All dogs were treated with imidocarb dipropionate (6.6 mg/kg of body weight), given intramuscularly twice at an interval of 14 days. This report presents the first molecular evidence of the occurrence of B. gibsoni and B. canis, confirmed by DNA sequencing, in sick dogs from Serbia.", publisher = "Akademiai Kiado Zrt, Budapest", journal = "Acta Veterinaria Hungarica", title = "Clinical babesiosis and molecular identification of babesia canis and babesia gibsoni infections in dogs from Serbia", volume = "63", number = "2", pages = "199-208", doi = "10.1556/AVet.2015.017" }
Davitkov, D., Vučićević, M., Stevanović, J., Krstić, V., Tomanović, S., Glavinić, U.,& Stanimirović, Z.. (2015). Clinical babesiosis and molecular identification of babesia canis and babesia gibsoni infections in dogs from Serbia. in Acta Veterinaria Hungarica Akademiai Kiado Zrt, Budapest., 63(2), 199-208. https://doi.org/10.1556/AVet.2015.017
Davitkov D, Vučićević M, Stevanović J, Krstić V, Tomanović S, Glavinić U, Stanimirović Z. Clinical babesiosis and molecular identification of babesia canis and babesia gibsoni infections in dogs from Serbia. in Acta Veterinaria Hungarica. 2015;63(2):199-208. doi:10.1556/AVet.2015.017 .
Davitkov, Darko, Vučićević, Miloš, Stevanović, Jevrosima, Krstić, Vanja, Tomanović, Snežana, Glavinić, Uroš, Stanimirović, Zoran, "Clinical babesiosis and molecular identification of babesia canis and babesia gibsoni infections in dogs from Serbia" in Acta Veterinaria Hungarica, 63, no. 2 (2015):199-208, https://doi.org/10.1556/AVet.2015.017 . .