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dc.creatorOzvegy, Jozsef
dc.creatorMarinković, Darko
dc.creatorVučićević, Miloš
dc.creatorGajić, Bojan
dc.creatorStevanović, Jevrosima
dc.creatorKrnjaić, Dejan
dc.creatorAleksić-Kovačević, Sanja
dc.date.accessioned2020-06-03T14:02:59Z
dc.date.available2020-06-03T14:02:59Z
dc.date.issued2015
dc.identifier.issn0567-8315
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1309
dc.description.abstractBlood smears stained with Diff Quick are the initial tool for cytological diagnosis of Haemogregarina spp. However, the development of sensitive and specific molecular methods enabled the detection and identification of parasites in the sample and to clarify the evolutionary relationships of adeleorinid parasites within the Apicomplexa. The current study was attempted in order to perform cytological investigation and molecular identification of the hemoparasites in thirty European pond turtles (Emys orbicularis) from the quarantine section at Belgrade Zoo, which have been found in poor health condition with massive skin hemorrhages, based on intraerythrocytic parasitic forms on hematological smears and 18S rDNA sequence, respectively. Different life cycle stages of the Haemogregarina sp. were noticed within the erythrocytes in the peripheral blood. Biochemical analysis indicated lower values of AST and iron in most of the infected turtles while hematological analysis showed a changed hematocrit value, a decrease in the number of red blood cells and low hemoglobin levels. Amplifications of the 18S rDNA sequence of Haemogregarina were detected in 30/30 (100%) turtles with clinical symptoms. The identity of PCR products was confirmed by direct DNA sequencing. Future research concerning H. stepanowi in Serbia should be applied to its definitive host-the leech.en
dc.publisherUniverzitet u Beogradu - Fakultet veterinarske medicine, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46002/RS//
dc.rightsopenAccess
dc.sourceActa veterinaria - Beograd
dc.subject18S rDNAen
dc.subjectEmys orbicularisen
dc.subjectHaemogregarina stepanowien
dc.subjectSerbiaen
dc.subjectcytological investigationen
dc.titleCytological and molecular identification of haemogregarina stepanowi in blood samples of the European pond turtle (emys orbicularis) from quarantine at Belgrade zooen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractГајић, Бојан; Озвегy, Јозсеф; Маринковић, Дарко; Aлексић-Ковачевић, Сања; Вучићевић, Милош; Стевановић, Јевросима; Крњаић, Дејан;
dc.citation.volume65
dc.citation.issue4
dc.citation.spage443
dc.citation.epage453
dc.citation.other65(4): 443-453
dc.citation.rankM22
dc.identifier.wos000367467000001
dc.identifier.doi10.1515/acve-2015-0037
dc.identifier.scopus2-s2.0-84955457760
dc.identifier.fulltexthttp://veterinar.vet.bg.ac.rs/bitstream/id/274/1308.pdf
dc.identifier.rcubconv_2121
dc.type.versionpublishedVersion


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