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Prisustvo sojeva stafilokoka rezistentnih na meticilin kod studenata Fakulteta veterinarske medicine Univerziteta u Beogradu

dc.creatorRađenović, Milan
dc.creatorAšanin, Jelena
dc.creatorAksentijević, Ksenija
dc.creatorMišić, Dušan
dc.date.accessioned2020-06-03T14:04:07Z
dc.date.available2020-06-03T14:04:07Z
dc.date.issued2016
dc.identifier.issn1820-9955
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1325
dc.description.abstractResistance to methicillin in staphylococci is considered to be one of the most dangerous forms of bacterial resistances to antibiotics. Methicillinresistant staphylococci (MRS) are zoonotic agents which cause local and systemic infections in humans and animals, often with a fatal outcome due to the absence of adequate antibiotic therapy. People colonized with strains of MRS are asymptomatic carriers and reservoirs of these strains in human populations. The aim of this research was to determine the prevalence of strains of MRS among clinically healthy students of the Faculty of Veterinary Medicine in Belgrade. The study was conducted on 100 volunteers: 62 males and 38 females. Given that staphylococci are expected to be found in the highest percentage in the nose and on the armpit skin, the swabs were taken from these regions of each person. Blood agar was innoculated immediately on taking the swabs After the incubation and isolation, the staphylococci were identified to species level. Their susceptibility to methicillin was tested in a disk-diffusion test with cefoxitin. All strains which were found to be resistant to cefoxitin were investigated for the presence of mecA gene with PCR. Staphylococci were isolated in 146 out of the 200 swabs taken: there were 79 nose swabs and 67 axillar swabs positive for these bacteria. Seventeen isolates were resistant to cefoxitin and the presence of the mecA gene was confirmed in seven, four of which were taken from the nose and three from the axillary region. The results of this research show that, being 6%, the prevalence of mecA-positive staphylococci in the population of clinically healthy students of veterinary medicine is significant. The percentage of methicillin-resistant staphylococci was higher in nose than in the axillar region of the students.en
dc.description.abstractRezistencija na meticilin kod stafilokoka smatra se jednim od najopasnijih oblika rezistencije bakterija na antibiotike. Sojevi meticilin rezistentnih stafilokoka (MRS) pripadaju zoonotskim agensima i uzročnici su lokalnih i sistemskih infekcija kod ljudi i životinja, često sa fatalnim ishodom zbog nedostatka efikasne terapije. Ljudi kolonizovani sojevima MRS su asimptomatski nosioci i predstavljaju rezervoare ovih sojeva u humanoj populaciji. Cilj ovog istraživanja je bio da se utvrdi prevalencija izolata MRS kod klinički zdravih studenata Fakulteta veterinarske medicine u Beogradu. U istraživanje je bilo uključeno 100 studenata - dobrovoljaca, 62 muškog i 38 ženskog pola. Brisevi su uzimani iz nosa i pazuha zato što su stafilokoke u najvećem procentu zastupljene u tim regijama. Brisevi su odmah zasejavani na krvni agar. Nakon inkubacije i izolacije, izvršena je identifikacija stafilokoka do vrste. Ispitivanje osetljivosti stafilokoka na meticilin izvedeno je primenom disk difuzione metode sa cefoksitinom. Svi sojevi rezistentni na cefoksitin ispitani su na prisustvo mecA gena metodom lančane reakcije polimeraze (PCR). Od ukupno 200 uzetih briseva izolovano je 146 izolata stafilokoka, 79 iz briseva nosa i 67 iz briseva pazušne regije. Kod 17 izolata ustanovljena je rezistencija na cefoksitin, a kod 7 je utvrđeno prisustvo mecA gena. Četiri mecA pozitivna izolata su poticala iz briseva nosa, a 3 sa kože pazušne regije. Utvrđena prevalencija meticilin-rezistentnih stafilokoka kod klinički zdravih studenata Fakulteta veterinarske medicine od 6%, procenjena je kao značajna. Veća učestalost stafilokoka rezistentnih na meticilin ustanovljena je na sluzokoži nosa u odnosu na kožu pazuha.sr
dc.publisherNaučni institut za veterinarstvo "Novi Sad", Novi Sad
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31079/RS//
dc.rightsopenAccess
dc.sourceArhiv veterinarske medicine
dc.subjectstaphylococcien
dc.subjectmethicillin resistanceen
dc.subjectprevalenceen
dc.subjectasymptomatic carriersen
dc.subjectstafilokokesr
dc.subjectrezistencija na meticilinsr
dc.subjectprevalencijasr
dc.subjectasimptomatski nosiocisr
dc.titleInvestigation of presence of methicillin resistant staphylococci in students of the Faculty of Veterinary Medicine at the University of Belgradeen
dc.titlePrisustvo sojeva stafilokoka rezistentnih na meticilin kod studenata Fakulteta veterinarske medicine Univerziteta u Beogradusr
dc.typearticle
dc.rights.licenseARR
dcterms.abstractРађеновић, Милан; Aксентијевић, Ксенија; Мишић, Душан; Aшанин, Јелена; Присуство сојева стафилокока резистентних на метицилин код студената Факултета ветеринарске медицине Универзитета у Београду; Присуство сојева стафилокока резистентних на метицилин код студената Факултета ветеринарске медицине Универзитета у Београду;
dc.citation.volume9
dc.citation.issue2
dc.citation.spage17
dc.citation.epage28
dc.citation.other9(2): 17-28
dc.citation.rankM51
dc.identifier.fulltexthttp://veterinar.vet.bg.ac.rs/bitstream/id/291/1324.pdf
dc.identifier.rcubconv_1215
dc.type.versionpublishedVersion


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