Examination of the influence of different adjuvants on the immunogenicity of vaccines against parainfluenza 3 virus in cattle

Abstract

The objective of our research was to examine the influence of different adjuvants on immunogenicity of vaccine against parainfluenza 3 virus in cattle. In the research there was used the commercial vaccine Respi-ol against PI3 virus in cattle, produced by VZS from Subotica as well as the experimental vaccine against the same virus prepared with completed adjuvant Emulsigen. The research was performed on two experimental groups of 12 calves each. The animals were immunized twice, in the interval of 21 days. The third group of 6 calves was a control. The results of the research showed that a smaller amount of adjuvent Emulsigen per vaccine dose provided a satisfactory height of titre of virus neu­tralizing antibodies in the blood serum samples of vaccinated calves in regard to prescribed greater amount of standard oil adjuvant contained in commercial Respi-ol vaccine.

Cilj naših istraživanja je bilo ispitivanje uticaja različitih adjuvanasa na imunogenost vakcine protiv parainfluence virusa 3 kod goveda. U ispitivanjima je korišćena komercijalna vakcina Respi-ol protiv virusa PI3 goveda, proizvođača VZS iz Subotice i eksperimentalna vakcina protiv navedenog virusa pripremljena sa gotovim adjuvansom Emulsigen-om. Ispitivanja su vršena na dve ogledne grupe teladi od po 12 životinja koje su dvokratno imunizovane u razmaku od 21 dan. Treća grupa teladi od ukupno 6 životinja je služila kao kontrola u ispitivanjima. Rezultati izvršenih ispitivanja su pokazali da je manja količina adjuvansa Emulsigen-a po dozi vakcine omogućavala postizanje zadovoljavajuće visine titra virus neutralizujućih antitela u uzorcima krvnog seruma vakcinisanih teladi u poređenju sa propisanom, većom količinom standardnog uljnog adjuvansa sadržanog u komercijalnoj vakcini Respi-ol.