L-arginine-NO system participates in the analgesic effect of flunixin meglumine in the rat
Učešće L-arginin-NO sistema u analgetičkom efektu fluniksin meglumina kod pacova
Abstract
This study investigated whether the L-arginine-NO system participates in the analgesic effect of flunixin meglumine in the rat. Hyperalgesia was induced by intraplantar (i.pl.) administration of carrageenan (500 μg) into the rat's hind paw. Electronic von Frey apparatus was used to determine paw withdrawal threshold induced by pressure as the painful stimulus, measured in grams (g). Flunixin meglumine (FM; 0.09-0.1 mg/kg; s.c.) and NG-nitro-L-arginine methyl ester (L-NAME; 10 mg/kg; i.p.), given separately as a pre-treatment, i.e. 15 min before i.pl. injection of carrageenan, produced a significant antinociception. When FM (0.09 mg/kg) and a sub-effective dose of L-NAME (5 mg/kg) were co-administered, the antinociceptive effect was significantly increased in comparison with the effect of FM alone. L-arginine (L-ARG;10 mg/kg; i.p.) itself did not produce significant effect on carrageenan-induced hyperalgesia, but significantly reduced the antinociceptive effects of both FM and FM + L-NA...ME combination. The inhibition of the production of NO might be involved in the mechanism of the analgesic effect of FM.
Ova studija ispituje da li L-arginin-NO sistem učestvuju u analgetskom dejstvu fluniksin meglumina kod pacova. Hiperalgezija je izazvana intraplantarnom (i.pl.) aplikacijom karagenina (500 μg) u zadnju šapu pacova. Elektronski von Frey aparat se koristi za određivanje praga osetljivosti šape izazvane pritiskom kao bolnim stimulusom, i meri se u gramima (g). Fluniksin meglumin (FM; 0,09-0,1 mg/kg, s.c.) i NG-nitro-L-arginin metil estar (L-NAME; 10 mg/kg, i.p.), dati odvojeno u pretretmanu, tj. 15 min pre i.pl. aplikacije karagenina, prouzrokuju značajnu antinocicepciju. Kada se FM (0,09 mg/ kg) i L-NAME u sub-efektivnoj dozi (5 mg/kg) primene zajedno, antinociceptivni efekat se značajno povećava u odnosu na efekat primene samog FM. L-arginin (10 mg/ kg; i.p.) sam ne deluje na hiperalgeziju izazvanu karageninom, ali značajno smanjuje antinociceptivno dejstvo FM i kombinacije FM + L-NAME. Inhibicija produkcije NO-a može biti uključena u mehanizam analgetičkog efekta FM.
Keywords:
Flunixin meglumine / L-NAME / L-arginine / carrageenan / hyperalgesiaSource:
Acta Veterinaria-Beograd, 2016, 66, 1, 103-114Publisher:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
DOI: 10.1515/acve-2016-0008
ISSN: 0567-8315
WoS: 000377780400008
Scopus: 2-s2.0-84962909560
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Fakultet veterinarske medicineTY - JOUR AU - Milovanović, Mirjana AU - Vučković, Sonja AU - Prostran, Milica AU - Trailović, Saša AU - Jovanović, Milan PY - 2016 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1364 AB - This study investigated whether the L-arginine-NO system participates in the analgesic effect of flunixin meglumine in the rat. Hyperalgesia was induced by intraplantar (i.pl.) administration of carrageenan (500 μg) into the rat's hind paw. Electronic von Frey apparatus was used to determine paw withdrawal threshold induced by pressure as the painful stimulus, measured in grams (g). Flunixin meglumine (FM; 0.09-0.1 mg/kg; s.c.) and NG-nitro-L-arginine methyl ester (L-NAME; 10 mg/kg; i.p.), given separately as a pre-treatment, i.e. 15 min before i.pl. injection of carrageenan, produced a significant antinociception. When FM (0.09 mg/kg) and a sub-effective dose of L-NAME (5 mg/kg) were co-administered, the antinociceptive effect was significantly increased in comparison with the effect of FM alone. L-arginine (L-ARG;10 mg/kg; i.p.) itself did not produce significant effect on carrageenan-induced hyperalgesia, but significantly reduced the antinociceptive effects of both FM and FM + L-NAME combination. The inhibition of the production of NO might be involved in the mechanism of the analgesic effect of FM. AB - Ova studija ispituje da li L-arginin-NO sistem učestvuju u analgetskom dejstvu fluniksin meglumina kod pacova. Hiperalgezija je izazvana intraplantarnom (i.pl.) aplikacijom karagenina (500 μg) u zadnju šapu pacova. Elektronski von Frey aparat se koristi za određivanje praga osetljivosti šape izazvane pritiskom kao bolnim stimulusom, i meri se u gramima (g). Fluniksin meglumin (FM; 0,09-0,1 mg/kg, s.c.) i NG-nitro-L-arginin metil estar (L-NAME; 10 mg/kg, i.p.), dati odvojeno u pretretmanu, tj. 15 min pre i.pl. aplikacije karagenina, prouzrokuju značajnu antinocicepciju. Kada se FM (0,09 mg/ kg) i L-NAME u sub-efektivnoj dozi (5 mg/kg) primene zajedno, antinociceptivni efekat se značajno povećava u odnosu na efekat primene samog FM. L-arginin (10 mg/ kg; i.p.) sam ne deluje na hiperalgeziju izazvanu karageninom, ali značajno smanjuje antinociceptivno dejstvo FM i kombinacije FM + L-NAME. Inhibicija produkcije NO-a može biti uključena u mehanizam analgetičkog efekta FM. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - L-arginine-NO system participates in the analgesic effect of flunixin meglumine in the rat T1 - Učešće L-arginin-NO sistema u analgetičkom efektu fluniksin meglumina kod pacova VL - 66 IS - 1 SP - 103 EP - 114 DO - 10.1515/acve-2016-0008 ER -
@article{ author = "Milovanović, Mirjana and Vučković, Sonja and Prostran, Milica and Trailović, Saša and Jovanović, Milan", year = "2016", abstract = "This study investigated whether the L-arginine-NO system participates in the analgesic effect of flunixin meglumine in the rat. Hyperalgesia was induced by intraplantar (i.pl.) administration of carrageenan (500 μg) into the rat's hind paw. Electronic von Frey apparatus was used to determine paw withdrawal threshold induced by pressure as the painful stimulus, measured in grams (g). Flunixin meglumine (FM; 0.09-0.1 mg/kg; s.c.) and NG-nitro-L-arginine methyl ester (L-NAME; 10 mg/kg; i.p.), given separately as a pre-treatment, i.e. 15 min before i.pl. injection of carrageenan, produced a significant antinociception. When FM (0.09 mg/kg) and a sub-effective dose of L-NAME (5 mg/kg) were co-administered, the antinociceptive effect was significantly increased in comparison with the effect of FM alone. L-arginine (L-ARG;10 mg/kg; i.p.) itself did not produce significant effect on carrageenan-induced hyperalgesia, but significantly reduced the antinociceptive effects of both FM and FM + L-NAME combination. The inhibition of the production of NO might be involved in the mechanism of the analgesic effect of FM., Ova studija ispituje da li L-arginin-NO sistem učestvuju u analgetskom dejstvu fluniksin meglumina kod pacova. Hiperalgezija je izazvana intraplantarnom (i.pl.) aplikacijom karagenina (500 μg) u zadnju šapu pacova. Elektronski von Frey aparat se koristi za određivanje praga osetljivosti šape izazvane pritiskom kao bolnim stimulusom, i meri se u gramima (g). Fluniksin meglumin (FM; 0,09-0,1 mg/kg, s.c.) i NG-nitro-L-arginin metil estar (L-NAME; 10 mg/kg, i.p.), dati odvojeno u pretretmanu, tj. 15 min pre i.pl. aplikacije karagenina, prouzrokuju značajnu antinocicepciju. Kada se FM (0,09 mg/ kg) i L-NAME u sub-efektivnoj dozi (5 mg/kg) primene zajedno, antinociceptivni efekat se značajno povećava u odnosu na efekat primene samog FM. L-arginin (10 mg/ kg; i.p.) sam ne deluje na hiperalgeziju izazvanu karageninom, ali značajno smanjuje antinociceptivno dejstvo FM i kombinacije FM + L-NAME. Inhibicija produkcije NO-a može biti uključena u mehanizam analgetičkog efekta FM.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "L-arginine-NO system participates in the analgesic effect of flunixin meglumine in the rat, Učešće L-arginin-NO sistema u analgetičkom efektu fluniksin meglumina kod pacova", volume = "66", number = "1", pages = "103-114", doi = "10.1515/acve-2016-0008" }
Milovanović, M., Vučković, S., Prostran, M., Trailović, S.,& Jovanović, M.. (2016). L-arginine-NO system participates in the analgesic effect of flunixin meglumine in the rat. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(1), 103-114. https://doi.org/10.1515/acve-2016-0008
Milovanović M, Vučković S, Prostran M, Trailović S, Jovanović M. L-arginine-NO system participates in the analgesic effect of flunixin meglumine in the rat. in Acta Veterinaria-Beograd. 2016;66(1):103-114. doi:10.1515/acve-2016-0008 .
Milovanović, Mirjana, Vučković, Sonja, Prostran, Milica, Trailović, Saša, Jovanović, Milan, "L-arginine-NO system participates in the analgesic effect of flunixin meglumine in the rat" in Acta Veterinaria-Beograd, 66, no. 1 (2016):103-114, https://doi.org/10.1515/acve-2016-0008 . .