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Ekspresija dejodinaza u placenti krava tretiranih sa propiltiouracilom (PTU) i aktivnost tireoidne osovine njihove teladi

dc.creatorKirovski, Danijela
dc.creatorDodovski, Petar
dc.creatorSavić, Đorđe
dc.creatorVujanac, Ivan
dc.creatorProdanović, Radiša
dc.creatorMirilović, Milorad
dc.creatorSladojević, Željko
dc.creatorĐorđević, Ana
dc.date.accessioned2020-06-03T14:07:01Z
dc.date.available2020-06-03T14:07:01Z
dc.date.issued2016
dc.identifier.issn0567-8315
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1366
dc.description.abstractThe aim of our study was to investigate if the thyroid axis of newborn calves is affected by prenatal application of propylthyouracil (PTU). The study included 20 late pregnant Holstein cows. One group (n=10) was treated with PTU (4 mg/kg of BW daily) from day 20 before expected calving until the day of calving. The other group (n=10) was non-treated. Placental samples of dams were obtained for measuring mRNA expression of iodothyronine deiodinases type I (D1), type II (D2) and type III (D3). After parturition calves were separated from the dams and included in the study. Blood samples were taken daily from each calf starting on the day of birth until day 7 of age. Blood T3, T4 and TSH concentrations were measured. PCR analysis of the placental tissue revealed an abundance of all three types of placental deiodinases in non-treated cows, and a significant elevation of mRNA levels for all three types of deiodinases after PTU treatment. Calves that originated from dams treated with PTU had significantly lower T3 and T4 and significantly higher TSH concentrations compared to non-treated calves during the first 2 days of life. Starting from day 4 until day 6 of life the opposite effect was observed meaning that calves prenatally exposed to PTU had significantly higher T3 and T4 and slightly lower TSH. Our study, for the first time, provides information related to iodothyronine deiodinases mRNA expression in bovine placenta, and confirm that PTU treatment of pregnant dams provokes depression of thyroid function in newborns during the first days of life.en
dc.description.abstractCilj ovog rada bio je da se ispita da li je tireoidna osovina novorođene teladi promenjena nakon prenatalne aplikacije propiltiouracila (PTU). U ogled je uključeno 20 krava holštajn rase u kasnom graviditetu. Prva grupa (n=10) je tretirana PTU (4 mg/kg telesne mase, dnevna doza) počevši od 20 dana pre očekivanog teljenja do dana teljenja. Druga grupa (n=10) nije tretirana. Uzorci placente krava uzimani su u cilju određivanja ekspresije iRNK dejodinaza tipa I (D1), tipa II (D2) i tipa III (D3). Nakon teljenja, telad su odvojena od majki i uključena u ispitivanja. Uzorci krvi su uzimani dnevno od svakog teleta počevši od dana rođenja do 7. dana života. U krvi su merene koncentracije T3, T4 i TSH. PCR analiza tkiva placente je pokazala zastupljenost sva tri tipa dejodinaza kod netretiranih krava, i značajno povećanje nivoa iRNK sva tri tipa dejodinaza nakon tretmana PTU. Telad koja su poticala od majki tretiranih PTU imala su značajno niže koncentracije T3 i T4 i značajno više koncentracije TSH u odnosu na telad poreklom od netretiranih majki, tokom prva dva dana života. U periodu od 4. do 6. dana života, utvrđen je suprotan efekat, što znači da su telad prenatalno izložena delovanju PTU imala značajno više koncentracije T3 i T4 i značajno niže koncentracije TSH u odnosu na netretiranu telad. Naša studija, po prvi put, daje informacije vezane za ekspresiju dejodinaza u bovinoj placenti i potvrđuje da tretman gravidnih majki sa PTU izaziva depresiju funkcije tireoidne osovine kod novorođenčadi tokom prva dva dana neonatalnog života.sr
dc.publisherUniverzitet u Beogradu - Fakultet veterinarske medicine, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46002/RS//
dc.rightsopenAccess
dc.sourceActa veterinaria - Beograd
dc.subjectcowsen
dc.subjectplacental deiodinasesen
dc.subjectneonateen
dc.subjectthyroid axisen
dc.subjectPTUen
dc.titlePlacental iodothyronine deiodinases expression in pregnant cows exposed to propylthiouracil (PTU) and thyroid axis activity of their calvesen
dc.titleEkspresija dejodinaza u placenti krava tretiranih sa propiltiouracilom (PTU) i aktivnost tireoidne osovine njihove teladisr
dc.typearticle
dc.rights.licenseARR
dcterms.abstractКировски, Данијела; Ђорђевић, Aна; Сладојевић, Жељко; Савић, Ђорђе; Мириловић, Милорад; Продановић, Радиша; Вујанац, Иван; Додовски, Петар; Експресија дејодиназа у плаценти крава третираних са пропилтиоурацилом (ПТУ) и активност тиреоидне осовине њихове телади; Експресија дејодиназа у плаценти крава третираних са пропилтиоурацилом (ПТУ) и активност тиреоидне осовине њихове телади;
dc.citation.volume66
dc.citation.issue1
dc.citation.spage61
dc.citation.epage75
dc.citation.other66(1): 61-75
dc.citation.rankM22
dc.identifier.wos000377780400005
dc.identifier.doi10.1515/acve-2016-0005
dc.identifier.scopus2-s2.0-84962787131
dc.identifier.fulltexthttp://veterinar.vet.bg.ac.rs/bitstream/id/332/1365.pdf
dc.identifier.rcubconv_842
dc.type.versionpublishedVersion


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