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dc.creatorColić, M
dc.creatorĆupić, Vitomir
dc.creatorPavicić, L
dc.creatorVučević, Dragana
dc.creatorVaragić, Vladislav M.
dc.date.accessioned2020-06-03T12:40:58Z
dc.date.available2020-06-03T12:40:58Z
dc.date.issued2000
dc.identifier.issn0379-0355
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/137
dc.description.abstractThe effect of xylazine, an alpha (2)-adrenergic agonist, on proliferation of rat thymocytes in vivo and in vitro was examined. It was found that the agonist administered to rats in vivo at doses of 2.5 mg/kg and 5 mg/kg stimulated thymocyte proliferation to suboptimal (0.625 mug/ml) concentrations of concanavalin A (Con A). A similar effect was confirmed in vitro when lower concentrations of xylazine (5 muM) were added to cultures of thymic cells from intact animals in the presence of both suboptimal and optimal (2.5 mug/ml) Con A concentrations. Higher doses in vivo (25 mg/kg) and in vitro (50 muM, 100 muM and 250 muM) significantly inhibited proliferation of thymocytes to Con A. The phenomenon was followed by a decrease in interleukin-2 (IL-2) production (in vivo and in vitro) and down-regulation of IL-2 receptor alpha (IL-2R alpha) expression (in vitro). The exogenous IL-2 completely restored the inhibitory effect of xylazine in vivo was observed. Stimulatory effect of xylazine on proliferation of thymocytes was probably mediated through alpha (2)-adrenoreceptors since it was blocked by yohimbine, an alpha (2)-adrenoreceptor antagonist. It seems that the pathways involved in inhibition of thymocyte proliferation by xylazine are more complex because the xylazine-suppressed thymocyte proliferation was potentiated by yohimine.en
dc.publisherProus Science, Sau-Thomson Reuters, Barcelona
dc.rightsrestrictedAccess
dc.sourceMethods and Findings in Experimental and Clinical Pharmacology
dc.subjectalpha(2)-adrenergic agonisten
dc.subjectthymocytesen
dc.subjectproliferationen
dc.subjectinterleukin-2 productionen
dc.subjectIL-2R alpha expressionen
dc.titleXylazine, an alpha(2)-adrenergic agonist, modulates proliferation of rat thymocytes in vivo and in vitroen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractВучевић, Драгана; Павицић, Л; Цолић, М; Варагић, Владислав М.; Ћупић, Витомир;
dc.citation.volume22
dc.citation.issue7
dc.citation.spage557
dc.citation.epage562
dc.citation.other22(7): 557-562
dc.citation.rankM23
dc.identifier.wos000166132700004
dc.identifier.pmid11196343
dc.identifier.rcubconv_1362
dc.type.versionpublishedVersion


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