Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product
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AuthorsŽugić, Ana R.
Lunter, Dominique Jasmin
Tadić, Vanja M.
Article (Published version)
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Topical treatment of skin infections is often limited by drawbacks related to both antimicrobial agents and their vehicles. In addition, considering the growing promotion of natural therapeutic products, our objective was to develop and evaluate naturally-based emulsion system, as prospective topical formulation for skin infections-treatment. Therefore, alkyl polyglucoside surfactants were used for stabilization of a vehicle serving as potential carrier for supercritical CO2-extract of Usnea barbata, lichen with well-documented antimicrobial activity, incorporated using two protocols and three concentrations. Comprehensive physicochemical characterization suggested possible involvement of extracts particles in stabilization of the investigated system. Raman spectral imaging served as the key method in disclosing extracts particles potential to participate in the microstructure of the tested emulsion system via three mechanisms: (1) particle-particle aggregation, (2) adsorption at the o...il-water interface and (3) hydrophobic particle-surfactant interactions. Stated extract-vehicle interaction proved to be correlated to the preparation procedure and extract concentration on one hand and to affect the physicochemical and biopharmaceutical features of investigated system, on the other hand. Thereafter, formulation with the best preliminary stability and liberation profile was selected for further efficiency and in vivo skin irritation potential evaluation, implying pertinent in vitro antimicrobial activity against G+ bacteria and overall satisfying preliminary safety profile.
Keywords:Antimicrobial activity / biopharmaceutical characterization / colloidal structure / extract-vehicle interaction / physicochemical stability / skin performance
Source:Pharmaceutical Development and Technology, 2016, 21, 5, 563-575
- Taylor & Francis Ltd, Abingdon
- German Academic Exchange Service Grant [A/11/84475]
- Functional physiologically active plant materials with additional values for application in pharmaceutical and food industry (RS-45017)
- Development of micro- and nanosystems as carriers for drugs with anti-inflammatory effect and methods for their characterization (RS-34031)