Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product
Само за регистроване кориснике
2016
Аутори
Žugić, Ana R.Lunter, Dominique Jasmin
Daniels, Rolf
Pantelić, Ivana
Tasic-Kostov, Marija
Tadić, Vanja M.
Mišić, Dušan
Arsić, Ivana
Savić, Snežana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Topical treatment of skin infections is often limited by drawbacks related to both antimicrobial agents and their vehicles. In addition, considering the growing promotion of natural therapeutic products, our objective was to develop and evaluate naturally-based emulsion system, as prospective topical formulation for skin infections-treatment. Therefore, alkyl polyglucoside surfactants were used for stabilization of a vehicle serving as potential carrier for supercritical CO2-extract of Usnea barbata, lichen with well-documented antimicrobial activity, incorporated using two protocols and three concentrations. Comprehensive physicochemical characterization suggested possible involvement of extracts particles in stabilization of the investigated system. Raman spectral imaging served as the key method in disclosing extracts particles potential to participate in the microstructure of the tested emulsion system via three mechanisms: (1) particle-particle aggregation, (2) adsorption at the o...il-water interface and (3) hydrophobic particle-surfactant interactions. Stated extract-vehicle interaction proved to be correlated to the preparation procedure and extract concentration on one hand and to affect the physicochemical and biopharmaceutical features of investigated system, on the other hand. Thereafter, formulation with the best preliminary stability and liberation profile was selected for further efficiency and in vivo skin irritation potential evaluation, implying pertinent in vitro antimicrobial activity against G+ bacteria and overall satisfying preliminary safety profile.
Кључне речи:
Antimicrobial activity / biopharmaceutical characterization / colloidal structure / extract-vehicle interaction / physicochemical stability / skin performanceИзвор:
Pharmaceutical Development and Technology, 2016, 21, 5, 563-575Издавач:
- Taylor & Francis Ltd, Abingdon
Финансирање / пројекти:
- German Academic Exchange Service Grant [A/11/84475]
- Функционални физиолошки активни биљни материјали са додатом вредношћу за примену у фармацеутској и прехрамбеној индустрији (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-45017)
- Развој микро- и наносистема као носача за лекове са антиинфламаторним деловањем и метода за њихову карактеризацију (RS-MESTD-Technological Development (TD or TR)-34031)
DOI: 10.3109/10837450.2015.1026606
ISSN: 1083-7450
PubMed: 26135231
WoS: 000375462900006
Scopus: 2-s2.0-84969244162
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Žugić, Ana R. AU - Lunter, Dominique Jasmin AU - Daniels, Rolf AU - Pantelić, Ivana AU - Tasic-Kostov, Marija AU - Tadić, Vanja M. AU - Mišić, Dušan AU - Arsić, Ivana AU - Savić, Snežana PY - 2016 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1390 AB - Topical treatment of skin infections is often limited by drawbacks related to both antimicrobial agents and their vehicles. In addition, considering the growing promotion of natural therapeutic products, our objective was to develop and evaluate naturally-based emulsion system, as prospective topical formulation for skin infections-treatment. Therefore, alkyl polyglucoside surfactants were used for stabilization of a vehicle serving as potential carrier for supercritical CO2-extract of Usnea barbata, lichen with well-documented antimicrobial activity, incorporated using two protocols and three concentrations. Comprehensive physicochemical characterization suggested possible involvement of extracts particles in stabilization of the investigated system. Raman spectral imaging served as the key method in disclosing extracts particles potential to participate in the microstructure of the tested emulsion system via three mechanisms: (1) particle-particle aggregation, (2) adsorption at the oil-water interface and (3) hydrophobic particle-surfactant interactions. Stated extract-vehicle interaction proved to be correlated to the preparation procedure and extract concentration on one hand and to affect the physicochemical and biopharmaceutical features of investigated system, on the other hand. Thereafter, formulation with the best preliminary stability and liberation profile was selected for further efficiency and in vivo skin irritation potential evaluation, implying pertinent in vitro antimicrobial activity against G+ bacteria and overall satisfying preliminary safety profile. PB - Taylor & Francis Ltd, Abingdon T2 - Pharmaceutical Development and Technology T1 - Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product VL - 21 IS - 5 SP - 563 EP - 575 DO - 10.3109/10837450.2015.1026606 ER -
@article{ author = "Žugić, Ana R. and Lunter, Dominique Jasmin and Daniels, Rolf and Pantelić, Ivana and Tasic-Kostov, Marija and Tadić, Vanja M. and Mišić, Dušan and Arsić, Ivana and Savić, Snežana", year = "2016", abstract = "Topical treatment of skin infections is often limited by drawbacks related to both antimicrobial agents and their vehicles. In addition, considering the growing promotion of natural therapeutic products, our objective was to develop and evaluate naturally-based emulsion system, as prospective topical formulation for skin infections-treatment. Therefore, alkyl polyglucoside surfactants were used for stabilization of a vehicle serving as potential carrier for supercritical CO2-extract of Usnea barbata, lichen with well-documented antimicrobial activity, incorporated using two protocols and three concentrations. Comprehensive physicochemical characterization suggested possible involvement of extracts particles in stabilization of the investigated system. Raman spectral imaging served as the key method in disclosing extracts particles potential to participate in the microstructure of the tested emulsion system via three mechanisms: (1) particle-particle aggregation, (2) adsorption at the oil-water interface and (3) hydrophobic particle-surfactant interactions. Stated extract-vehicle interaction proved to be correlated to the preparation procedure and extract concentration on one hand and to affect the physicochemical and biopharmaceutical features of investigated system, on the other hand. Thereafter, formulation with the best preliminary stability and liberation profile was selected for further efficiency and in vivo skin irritation potential evaluation, implying pertinent in vitro antimicrobial activity against G+ bacteria and overall satisfying preliminary safety profile.", publisher = "Taylor & Francis Ltd, Abingdon", journal = "Pharmaceutical Development and Technology", title = "Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product", volume = "21", number = "5", pages = "563-575", doi = "10.3109/10837450.2015.1026606" }
Žugić, A. R., Lunter, D. J., Daniels, R., Pantelić, I., Tasic-Kostov, M., Tadić, V. M., Mišić, D., Arsić, I.,& Savić, S.. (2016). Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product. in Pharmaceutical Development and Technology Taylor & Francis Ltd, Abingdon., 21(5), 563-575. https://doi.org/10.3109/10837450.2015.1026606
Žugić AR, Lunter DJ, Daniels R, Pantelić I, Tasic-Kostov M, Tadić VM, Mišić D, Arsić I, Savić S. Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product. in Pharmaceutical Development and Technology. 2016;21(5):563-575. doi:10.3109/10837450.2015.1026606 .
Žugić, Ana R., Lunter, Dominique Jasmin, Daniels, Rolf, Pantelić, Ivana, Tasic-Kostov, Marija, Tadić, Vanja M., Mišić, Dušan, Arsić, Ivana, Savić, Snežana, "Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product" in Pharmaceutical Development and Technology, 21, no. 5 (2016):563-575, https://doi.org/10.3109/10837450.2015.1026606 . .