The application of molecular methods in the diagnostics of infection of swine caused by porcine Circovirus 2

Abstract

Porcine circovirus 2 (PCV2) belongs to the family Circoviridae, genus Circovirus. Infection of swine caused by this virus is manifested in several different clinical forms, leading to significant economic losses in swine production worldwide. For this reason, prompt and precise diagnostics of this swine infection is of great importance. For this purpose today there are used molecular methods of virological diagnostics such as polymerase chain reaction (PCR), and real-time method PCR, that is direct sequencing method by Sanger.

Svinjski cirkovirus 2 (PCV2) pripada familiji Circoviridae i rodu Circovirus. Infekcija svinja izazvana ovim virusom se ispoljava u više različitih kliničkih formi dovodeći do značajnih ekonomskih gubitaka u proizvodnji svinja širom sveta. Iz navedenog razloga brza i precizna dijagnostika ove infekcije svinja je od izuzetnog značaja. U te svrhe se danas koriste molekularne metode virusološke dijagnostike kao što su lančana reakcija polimeraze (PCR), zatim metoda real-time PCR, odnosno metoda dierktnog sekvenciranja po Sanger-u.