Molecular detection and prevalence of Theileria equi and Babesia caballi in horses of central Balkan
Samo za registrovane korisnike
2016
Autori
Davitkov, DarkoVučićević, Miloš
Stevanović, Jevrosima
Krstić, Vanja
Slijepcević, Dajana
Glavinić, Uroš
Stanimirović, Zoran
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Equine piroplasmosis is significant tick-borne disease with wide distribution. The prevalence of equine piroplasmosis in Serbia, Montenegro and Bosnia and Herzegovina is unknown. In aim to obtain a first insight into the prevalence we performed molecular epidemiological study which included 142 horses, on seven locations in these three countries. We first performed PCR for the detection of a 450bp long section of the 18S rRNA of piroplasma-specific region. For all positive samples we have done multiplex PCR for the species detection. Species determination was further confirmed by sequencing PCR products of 10 randomly selected Theileria equi and all Babesia caballi samples. The overall prevalence rates in analysed region for T. equi and B. caballi were 22.5% and 2.1%, respectively. Possible risk factors (such as location, age, sex and activity) associated with PCR positivity were evaluated. Marked differences were found in prevalence between geographic areas. There was no significant a...ssociation between positivity and age group. T. equi was more prevalent in females and farming horses. This is the first report on the molecular survey of T. equi and B. caballi in central Balkan. Further prevalence studies on definitive host and vectors in this region are necessary.
Ključne reči:
Equine piroplasmosis / horses / PCR / Serbia / Montenegro / Bosnia and HerzegovinaIzvor:
Acta Parasitologica, 2016, 61, 2, 337-342Izdavač:
- Springer International Publishing Ag, Cham
Finansiranje / projekti:
- Molekularno-genetička i ekofiziološka istraživanja u zaštiti autohtonih animalnih genetičkih resursa, očuvanju dobrobiti, zdravlja i reprodukcije gajenih životinja i proizvodnji bezbedne hrane (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
DOI: 10.1515/ap-2016-0044
ISSN: 1230-2821
PubMed: 27078657
WoS: 000374972400016
Scopus: 2-s2.0-84964858185
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Davitkov, Darko AU - Vučićević, Miloš AU - Stevanović, Jevrosima AU - Krstić, Vanja AU - Slijepcević, Dajana AU - Glavinić, Uroš AU - Stanimirović, Zoran PY - 2016 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1393 AB - Equine piroplasmosis is significant tick-borne disease with wide distribution. The prevalence of equine piroplasmosis in Serbia, Montenegro and Bosnia and Herzegovina is unknown. In aim to obtain a first insight into the prevalence we performed molecular epidemiological study which included 142 horses, on seven locations in these three countries. We first performed PCR for the detection of a 450bp long section of the 18S rRNA of piroplasma-specific region. For all positive samples we have done multiplex PCR for the species detection. Species determination was further confirmed by sequencing PCR products of 10 randomly selected Theileria equi and all Babesia caballi samples. The overall prevalence rates in analysed region for T. equi and B. caballi were 22.5% and 2.1%, respectively. Possible risk factors (such as location, age, sex and activity) associated with PCR positivity were evaluated. Marked differences were found in prevalence between geographic areas. There was no significant association between positivity and age group. T. equi was more prevalent in females and farming horses. This is the first report on the molecular survey of T. equi and B. caballi in central Balkan. Further prevalence studies on definitive host and vectors in this region are necessary. PB - Springer International Publishing Ag, Cham T2 - Acta Parasitologica T1 - Molecular detection and prevalence of Theileria equi and Babesia caballi in horses of central Balkan VL - 61 IS - 2 SP - 337 EP - 342 DO - 10.1515/ap-2016-0044 ER -
@article{ author = "Davitkov, Darko and Vučićević, Miloš and Stevanović, Jevrosima and Krstić, Vanja and Slijepcević, Dajana and Glavinić, Uroš and Stanimirović, Zoran", year = "2016", abstract = "Equine piroplasmosis is significant tick-borne disease with wide distribution. The prevalence of equine piroplasmosis in Serbia, Montenegro and Bosnia and Herzegovina is unknown. In aim to obtain a first insight into the prevalence we performed molecular epidemiological study which included 142 horses, on seven locations in these three countries. We first performed PCR for the detection of a 450bp long section of the 18S rRNA of piroplasma-specific region. For all positive samples we have done multiplex PCR for the species detection. Species determination was further confirmed by sequencing PCR products of 10 randomly selected Theileria equi and all Babesia caballi samples. The overall prevalence rates in analysed region for T. equi and B. caballi were 22.5% and 2.1%, respectively. Possible risk factors (such as location, age, sex and activity) associated with PCR positivity were evaluated. Marked differences were found in prevalence between geographic areas. There was no significant association between positivity and age group. T. equi was more prevalent in females and farming horses. This is the first report on the molecular survey of T. equi and B. caballi in central Balkan. Further prevalence studies on definitive host and vectors in this region are necessary.", publisher = "Springer International Publishing Ag, Cham", journal = "Acta Parasitologica", title = "Molecular detection and prevalence of Theileria equi and Babesia caballi in horses of central Balkan", volume = "61", number = "2", pages = "337-342", doi = "10.1515/ap-2016-0044" }
Davitkov, D., Vučićević, M., Stevanović, J., Krstić, V., Slijepcević, D., Glavinić, U.,& Stanimirović, Z.. (2016). Molecular detection and prevalence of Theileria equi and Babesia caballi in horses of central Balkan. in Acta Parasitologica Springer International Publishing Ag, Cham., 61(2), 337-342. https://doi.org/10.1515/ap-2016-0044
Davitkov D, Vučićević M, Stevanović J, Krstić V, Slijepcević D, Glavinić U, Stanimirović Z. Molecular detection and prevalence of Theileria equi and Babesia caballi in horses of central Balkan. in Acta Parasitologica. 2016;61(2):337-342. doi:10.1515/ap-2016-0044 .
Davitkov, Darko, Vučićević, Miloš, Stevanović, Jevrosima, Krstić, Vanja, Slijepcević, Dajana, Glavinić, Uroš, Stanimirović, Zoran, "Molecular detection and prevalence of Theileria equi and Babesia caballi in horses of central Balkan" in Acta Parasitologica, 61, no. 2 (2016):337-342, https://doi.org/10.1515/ap-2016-0044 . .