Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression
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2016
Authors
Prodanović, RadišaKorićanac, Goran
Vujanac, Ivan
Đorđević, Ana
Pantelić, Marija
Romić, Snjezana
Stanimirović, Zoran
Kirovski, Danijela
Article (Published version)
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We investigated the hypothesis that obesity in dairy cows enhanced expression of proteins involved in hepatic fatty acid uptake and metabolism. Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups based on their body condition score (BCS) as optimal (3.25 <= BCS <= 3.5) and high (4.0 <= BCS <= 425). Intravenous glucose tolerance test (GTT) and liver biopsies were carried out at day 10 before calving. Blood samples were collected before (basal) and after glucose infusion, and glucose, insulin and non-esterified fatty acid (NEFA) levels were determined at each sample point. In addition, beta-hydroxybutyrate and triglycerides levels were measured in the basal samples. The liver biopsies were analyzed for total lipid content and protein expression of insulin receptor beta (IR beta), fatty acid translocase (FAT/CD36) and sterol regulatory element-binding protein-1 (SREBP-1). Basal glucose and insulin were higher in high-BCS cows, which coincided with higher circulating t...riglycerides and hepatic lipid content. Clearance rate and AUC for NEFA during GTT were higher in optimal-BCS cows. The development of insulin resistance and fatty liver in obese cows was paralleled by increased hepatic expression of the IR beta, CD36 and SREBP-1. These results suggest that increased expression of hepatic CD36 and SREBP-1 is relevant in the obesity-driven lipid accumulation in the liver of dairy cows during late gestation.
Keywords:
Dairy cows / Fatty liver / CD36 / Sterol regulatory element-binding protein-1Source:
Research in Veterinary Science, 2016, 107, 16-19Publisher:
- Elsevier Sci Ltd, Oxford
Funding / projects:
- Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
- Role of steroid hormones in neuroendocrine adaptation to stress and pathophysiology of metabolic syndrome - molecular mechanisms and clinical implications (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41009)
DOI: 10.1016/j.rvsc.2016.04.007
ISSN: 0034-5288
PubMed: 27473969
WoS: 000381541600003
Scopus: 2-s2.0-84969165526
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Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Prodanović, Radiša AU - Korićanac, Goran AU - Vujanac, Ivan AU - Đorđević, Ana AU - Pantelić, Marija AU - Romić, Snjezana AU - Stanimirović, Zoran AU - Kirovski, Danijela PY - 2016 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1396 AB - We investigated the hypothesis that obesity in dairy cows enhanced expression of proteins involved in hepatic fatty acid uptake and metabolism. Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups based on their body condition score (BCS) as optimal (3.25 <= BCS <= 3.5) and high (4.0 <= BCS <= 425). Intravenous glucose tolerance test (GTT) and liver biopsies were carried out at day 10 before calving. Blood samples were collected before (basal) and after glucose infusion, and glucose, insulin and non-esterified fatty acid (NEFA) levels were determined at each sample point. In addition, beta-hydroxybutyrate and triglycerides levels were measured in the basal samples. The liver biopsies were analyzed for total lipid content and protein expression of insulin receptor beta (IR beta), fatty acid translocase (FAT/CD36) and sterol regulatory element-binding protein-1 (SREBP-1). Basal glucose and insulin were higher in high-BCS cows, which coincided with higher circulating triglycerides and hepatic lipid content. Clearance rate and AUC for NEFA during GTT were higher in optimal-BCS cows. The development of insulin resistance and fatty liver in obese cows was paralleled by increased hepatic expression of the IR beta, CD36 and SREBP-1. These results suggest that increased expression of hepatic CD36 and SREBP-1 is relevant in the obesity-driven lipid accumulation in the liver of dairy cows during late gestation. PB - Elsevier Sci Ltd, Oxford T2 - Research in Veterinary Science T1 - Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression VL - 107 SP - 16 EP - 19 DO - 10.1016/j.rvsc.2016.04.007 ER -
@article{ author = "Prodanović, Radiša and Korićanac, Goran and Vujanac, Ivan and Đorđević, Ana and Pantelić, Marija and Romić, Snjezana and Stanimirović, Zoran and Kirovski, Danijela", year = "2016", abstract = "We investigated the hypothesis that obesity in dairy cows enhanced expression of proteins involved in hepatic fatty acid uptake and metabolism. Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups based on their body condition score (BCS) as optimal (3.25 <= BCS <= 3.5) and high (4.0 <= BCS <= 425). Intravenous glucose tolerance test (GTT) and liver biopsies were carried out at day 10 before calving. Blood samples were collected before (basal) and after glucose infusion, and glucose, insulin and non-esterified fatty acid (NEFA) levels were determined at each sample point. In addition, beta-hydroxybutyrate and triglycerides levels were measured in the basal samples. The liver biopsies were analyzed for total lipid content and protein expression of insulin receptor beta (IR beta), fatty acid translocase (FAT/CD36) and sterol regulatory element-binding protein-1 (SREBP-1). Basal glucose and insulin were higher in high-BCS cows, which coincided with higher circulating triglycerides and hepatic lipid content. Clearance rate and AUC for NEFA during GTT were higher in optimal-BCS cows. The development of insulin resistance and fatty liver in obese cows was paralleled by increased hepatic expression of the IR beta, CD36 and SREBP-1. These results suggest that increased expression of hepatic CD36 and SREBP-1 is relevant in the obesity-driven lipid accumulation in the liver of dairy cows during late gestation.", publisher = "Elsevier Sci Ltd, Oxford", journal = "Research in Veterinary Science", title = "Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression", volume = "107", pages = "16-19", doi = "10.1016/j.rvsc.2016.04.007" }
Prodanović, R., Korićanac, G., Vujanac, I., Đorđević, A., Pantelić, M., Romić, S., Stanimirović, Z.,& Kirovski, D.. (2016). Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression. in Research in Veterinary Science Elsevier Sci Ltd, Oxford., 107, 16-19. https://doi.org/10.1016/j.rvsc.2016.04.007
Prodanović R, Korićanac G, Vujanac I, Đorđević A, Pantelić M, Romić S, Stanimirović Z, Kirovski D. Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression. in Research in Veterinary Science. 2016;107:16-19. doi:10.1016/j.rvsc.2016.04.007 .
Prodanović, Radiša, Korićanac, Goran, Vujanac, Ivan, Đorđević, Ana, Pantelić, Marija, Romić, Snjezana, Stanimirović, Zoran, Kirovski, Danijela, "Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression" in Research in Veterinary Science, 107 (2016):16-19, https://doi.org/10.1016/j.rvsc.2016.04.007 . .