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dc.creatorBuac, Marijana
dc.creatorMojsilović, Slavko
dc.creatorMišić, Dušan
dc.creatorVuković, Dejan
dc.creatorSavić, Olivera
dc.creatorValčić, Olivera
dc.creatorMarković, Dragana
dc.creatorGvozdić, Dragan
dc.creatorIlić, Vesna
dc.creatorFratrić, Natalija
dc.date.accessioned2020-06-03T14:09:38Z
dc.date.available2020-06-03T14:09:38Z
dc.date.issued2016
dc.identifier.issn0034-5288
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1403
dc.description.abstractIn this work we studied if circulating immune complexes (CIC) of calves with bronchopneumonia have the capacity to modulate function of peripheral blood leukocytes of healthy cattle. CIC of three month old calves (6 healthy and 6 diseased) were isolated by PEG precipitation. Peripheral blood mononuclear cells (MNCs) and granulocytes from healthy calves and cows were the CIC responder cells in in vitro tests. The most remarkable increase of adhesiveness to polystyrene and ROS synthesis (assessed by NBT test) was detected in cows granulocytes stimulated with CIC of diseased calves. Results of MTT test showed that CIC of both healthy and diseased calves reduced granulocytes viability. The strongest effect of inhibition of cows granulocytes resulted from CIC of diseased calves. CIC only moderately reduced spontaneous viability of calves MNCs. Again, the strongest effect of CIC isolated from diseased calves was observed. In contrast to the low impact of CIC on non-stimulated cells, their inhibitory effect on viability of mitogen stimulated MNCs was very strong. With CFSE assay we showed that both types of CIC stimulated spontaneous, but inhibited mitogen induced proliferation of calves MNCs. Propidium iodide staining reviled that CIC increased apoptosis/necrosis of both non-stimulated and mitogen stimulated MNCs. CIC of both healthy and diseased calves modulated the function of peripheral blood MNCs and granulocytes, but a stronger effect of CIC of diseased calves was shown. The age of the donors (calves or cows) of the responder cells, and the activation state of these cells, were also of influence.en
dc.publisherElsevier Sci Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46002/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175062/RS//
dc.rightsrestrictedAccess
dc.sourceResearch in Veterinary Science
dc.subjectCalf bronchopneumoniaen
dc.subjectCirculating immune complexesen
dc.subjectPeripheral blood leukocytesen
dc.titleCirculating immune complexes of calves with bronchopneumonia modulate the function of peripheral blood leukocytes: In vitro evaluationen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractФратрић, Наталија; Савић, Оливера; Илић, Весна; Гвоздић, Драган; Мишић, Душан; Валчић, Оливера; Мојсиловић, Славко; Марковић, Драгана; Буац, Маријана; Вуковић, Дејан;
dc.citation.volume106
dc.citation.spage135
dc.citation.epage142
dc.citation.other106: 135-142
dc.citation.rankM22
dc.identifier.wos000377923400024
dc.identifier.doi10.1016/j.rvsc.2016.04.002
dc.identifier.pmid27234551
dc.identifier.scopus2-s2.0-84963699389
dc.identifier.rcubconv_2161
dc.type.versionpublishedVersion


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