Show simple item record

dc.creatorJovanović, Sofija
dc.creatorBallantyne, Thomas
dc.creatorDu, Qingyou
dc.creatorBlagojević, Miloš
dc.creatorJovanović, Aleksandar
dc.date.accessioned2020-06-03T14:10:29Z
dc.date.available2020-06-03T14:10:29Z
dc.date.issued2016
dc.identifier.issn1357-2725
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1415
dc.description.abstractATP-sensitive K+ (K-ATP) channels were originally described in cardiomyocytes, where physiological levels of intracellular ATP keep them in a closed state. Structurally, these channels are composed of pore-forming inward rectifier, Kir6.1 or Kir6.2, and a regulatory, ATP-binding subunit, SUR1, SUR2A or SUR2B. SUR1 and Kir6.2 form pancreatic type of KATP channels, SUR2A and Kir6.2 form cardiac type of KATP channels, SUR2B and Kir6.1 form vascular smooth muscle type of KATP channels. The presence of SUR2B has been described in cardiomyocytes, but its functional significance and role has remained unknown. Pretreatment with phenylephrine (100 nM) for 24 h increased mRNA levels of SUR2B and Kir6.2, without affecting those levels of SUR1, SUR2A and Kir6.1 in embryonic heart H9c2 cells. Such increase was associated with increased K+ current through KATP channels and Kir6.2/SUR2B protein complexes as revealed by whole cell patch clamp electrophysiology and immunoprecipitation/Western blotting respectively. Pretreatment with phenylephrine (100 nM) generated a cellular phenotype that acquired resistance to chemical hypoxia induced by 2,4-dinitrophenol (DNP; 10 mM), which was accompanied by increased in K+ current in response to DNP (10 mM). Cytoprotection afforded by phenylephrine (100 nM) was abolished by infection of H9c2 cells with adenovirus containing Kir6.2AFA, a mutant form of Kir6.2 with largely reduced K+ conductance. Taking all together, the present findings demonstrate that the activation of alpha 1-adrenoceptors up-regulates SUR2B/Kir6.2 to confer cardioprotection. This is the first account of possible physiological role of SUR2B in cardiomyocytes.en
dc.publisherPergamon-Elsevier Science Ltd, Oxford
dc.relationBritish Heart FoundationBritish Heart Foundation [PG/11/106/29235, PG/15/28/31384]
dc.relationBritish Heart FoundationBritish Heart Foundation [PG/15/28/31384]
dc.rightsopenAccess
dc.sourceInternational Journal of Biochemistry & Cell Biology
dc.subjectSUR2Ben
dc.subjectCardioprotectionen
dc.subjectPreconditioningen
dc.subjectCardiomyocytesen
dc.subjectH9c2 cellsen
dc.titlePhenylephrine preconditioning in embryonic heart H9c2 cells is mediated by up-regulation of SUR2B/Kir6.2: A first evidence for functional role of SUR2B in sarcolemmal K-ATP channels and cardioprotectionen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЈовановић, Aлександар; Ду, Qингyоу; Баллантyне, Тхомас; Благојевић, Милош; Јовановић, Софија;
dc.citation.volume70
dc.citation.spage23
dc.citation.epage28
dc.citation.other70: 23-28
dc.citation.rankM22
dc.identifier.wos000368869500003
dc.identifier.doi10.1016/j.biocel.2015.10.029
dc.identifier.pmid26556311
dc.identifier.scopus2-s2.0-84947266195
dc.identifier.fulltexthttp://veterinar.vet.bg.ac.rs/bitstream/id/381/1414.pdf
dc.identifier.rcubconv_2129
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record