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dc.creatorRadaković, Milena
dc.creatorDavitkov, Darko
dc.creatorBorozan, Sunčica
dc.creatorStojanović, S.
dc.creatorStevanović, Jevrosima
dc.creatorKrstić, Vanja
dc.creatorStanimirović, Zoran
dc.date.accessioned2020-06-03T14:10:54Z
dc.date.available2020-06-03T14:10:54Z
dc.date.issued2016
dc.identifier.issn1090-0233
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1421
dc.description.abstractThe aim of this study was to determine the concentrations of oxidative stress parameters and DNA damage in horses infected by Theileria equi. Initial screening of 110 horses with duplex PCR enabled the selection of 30 infected horses with T. equi and 30 free of infection (control). Specimens from the 60 horses were further analysed by determining the following oxidative stress parameters: extent of haemolysis (EH), plasma free haemoglobin (PHb), catalase (CAT), Cu,Zn superoxide dismutase (SOD1), paraoxonase (PON1), nitrite (NO2-), total nitrate and nitrite (NOx), malondialdehyde (MDA) and free thiol groups (-SH). In addition, relative distribution of lactate dehydrogenase (LDH1-LDH5) activity and the DNA-damaging effects of T. equi infection were evaluated. Compared to control horses, horses infected with T. equi had significantly higher SOD1 activities (P < 0.05) and PHb (P < 0.01), NO2- (P < 0.001), NOx (P < 0.05) and MDA concentrations (P < 0.001), and significantly-lower EH (P < 0.001), CAT (P < 0.01) and PON1 (P < 0.001) activities, and thiol group concentrations (P < 0.05). The comet assay demonstrated significantly increased DNA damage in T. equi infected cells compared to non-infected cells (P < 0.001). Infected horses had significantly increased LDH5 isoenzyme activities (P < 0.05). There was higher production of ROS/RNS in T. equi-infected horses, which resulted in changes in osmotic fragility, damage to lipids, proteins and DNA, haemolysis and hepatocellular damage. Oxidative stress in horses naturally infected with T equi could contribute to the pathogenesis of the infection.en
dc.publisherElsevier Sci Ltd, Oxford
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46002/RS//
dc.rightsrestrictedAccess
dc.sourceVeterinary Journal
dc.subjectDNA damageen
dc.subjectHorsesen
dc.subjectDuplex PCRen
dc.subjectOxidative stressen
dc.subjectTheileria equien
dc.titleOxidative stress and DNA damage in horses naturally infected with Theileria equien
dc.typearticle
dc.rights.licenseARR
dcterms.abstractБорозан, Сунчица; Стевановић, Јевросима; Крстић, Вања; Давитков, Дарко; Станимировић, Зоран; Стојановић, С.; Радаковић, Милена;
dc.citation.volume217
dc.citation.spage112
dc.citation.epage118
dc.citation.other217: 112-118
dc.citation.rankM21
dc.identifier.wos000388779400021
dc.identifier.doi10.1016/j.tvjl.2016.10.003
dc.identifier.pmid27810201
dc.identifier.scopus2-s2.0-84994017790
dc.identifier.rcubconv_2225
dc.type.versionpublishedVersion


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