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dc.creatorGajić, Bojan
dc.creatorStevanović, Jevrosima
dc.creatorRadulović, Željko
dc.creatorKulišić, Zoran
dc.creatorVejnović, Branislav
dc.creatorGlavinić, Uroš
dc.creatorStanimirović, Zoran
dc.date.accessioned2020-06-03T14:10:58Z
dc.date.available2020-06-03T14:10:58Z
dc.date.issued2016
dc.identifier.issn0168-8162
dc.identifier.urihttps://vet-erinar.vet.bg.ac.rs/handle/123456789/1422
dc.description.abstractIn the present study, amplification refractory mutation system (ARMS) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods were used for identification of recently described Serbia 1 (S1) and Peshter 1 (P1) mitochondrial haplotypes of Varroa destructor. Based on single nucleotide polymorphisms (SNPs) within cytochrome oxidase 1 (cox1) and cytochrome b (cytb) gene sequences, a total of 64 adult V. destructor females were analyzed from locations where the S1 and P1 haplotypes had been detected previously. Results of haplotype identification obtained by ARMS and PCR-RFLP methods were completely consistent with the sequencing data. Furthermore, in some analyzed samples the occurrence of site heteroplasmy at haplotype-defining sites was detected, as it was confirmed by double peaks in the sequence chromatograms. Neither mites with simultaneous nucleotide variability, nor those with combined SNP and heteroplasmy in cox1 and cytb were found. Given that this is the first occurrence of site heteroplasmy in V. destructor, the origin of this phenomenon and possible specific traits of heteroplasmic mites have yet to be determined.en
dc.publisherSpringer, Dordrecht
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46002/RS//
dc.rightsrestrictedAccess
dc.sourceExperimental and Applied Acarology
dc.subjectVarroa destructoren
dc.subjectHaplotypeen
dc.subjectmtDNA heteroplasmyen
dc.subjectARMSen
dc.subjectPCR-RFLPen
dc.titleHaplotype identification and detection of mitochondrial DNA heteroplasmy in Varroa destructor mites using ARMS and PCR-RFLP methodsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractГлавинић, Урош; Гајић, Бојан; Станимировић, Зоран; Вејновић, Бранислав; Кулишић, Зоран; Стевановић, Јевросима; Радуловић, Жељко;
dc.citation.volume70
dc.citation.issue3
dc.citation.spage287
dc.citation.epage297
dc.citation.other70(3): 287-297
dc.citation.rankM21
dc.identifier.wos000386075800003
dc.identifier.doi10.1007/s10493-016-0086-6
dc.identifier.pmid27631761
dc.identifier.scopus2-s2.0-84986267458
dc.type.versionpublishedVersion


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Приказ основних података о документу