Show simple item record

dc.creatorTrailović, Saša
dc.creatorZurovac-Sapundžić, Zorana
dc.creatorGruborović, S.
dc.creatorMarjanović, Đorđe
dc.creatorNedeljković-Trailović, Jelena
dc.date.accessioned2020-06-03T14:11:11Z
dc.date.available2020-06-03T14:11:11Z
dc.date.issued2016
dc.identifier.issn0022-149X
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1425
dc.description.abstractThe aim of this study was to determine the role in contractions of postsynaptic nicotinic acetylcholine (nACh) and gamma- aminobutyric acid (GABA) receptors, in the bag region of Ascaris suum muscle cells, as well as the role of synaptic receptors between interneurons and motor neurons in the dorsal and ventral nerve cord. We have measured the isometric contractions of isolated segments of A. suum, with or without the nerve cord (dorsal or ventral). Contractions were caused by increasing concentrations of ACh or by electrical field stimulation (EFS). Based on our results, the presence of the nerve cord is essential for the contractile effects of ACh. The EC50 value of ACh for innervated muscle strips was 10.88 mu M. Unlike intact (innervated) preparations, there was no contraction of the muscle flaps when the nerve cord was mechanically removed. Furthermore, continuous EFS produced stable contractions of innervated muscle strips, but they are not sensitive to mecamylamine (100 mu M). However, GABA (30 mu M) significantly inhibited the EFS- induced contractions. EFS with the same characteristics did not cause muscle contractions of denervated muscle strips, but EFS with a wider pulse induced the increasing of tone and irregular contractions. These contractions were completely insensitive to the effect of GABA. The EC50 for ACh did not differ between the dorsal and ventral segments (9.83 mu M and 9.45 mu M), while GABA exhibited features of competitive and noncompetitive antagonists, regardless of whether it acted on the dorsal or ventral segments of A. suum. It is obvious that drugs will be more effective if they act on both the synaptic and extrasynaptic nACh and GABA receptors.en
dc.publisherCambridge Univ Press, Cambridge
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31087/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Helminthology
dc.titlePresynaptic and postsynaptic regulation of muscle contractions in the ascarid nematode Ascaris suum: a target for drug actionen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractМарјановић, Ђорђе; Траиловић, Саша; Зуровац-Сапунджић, Зорана; Недељковић-Траиловић, Јелена; Груборовић, С.;
dc.citation.volume90
dc.citation.issue6
dc.citation.spage698
dc.citation.epage705
dc.citation.other90(6): 698-705
dc.citation.rankM22
dc.identifier.wos000387970900009
dc.identifier.doi10.1017/S0022149X15000978
dc.identifier.pmid26610818
dc.identifier.scopus2-s2.0-84948680131
dc.identifier.rcubconv_2217
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record