Detection and analysis of new psittacine beak and feather disease virus (PBFDv) nucleotide sequences
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2017
Authors
Vučićević, MilošVučićević, Ivana
Davitkov, Darko
Davitkov, Dajana
Stevanović, Jevrosima
Resanović, Radmila
Stanimirović, Zoran
Article (Published version)
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Show full item recordAbstract
Psittacine beak and feather disease (PBFD) affects a large number of Psittaciformes species. In this study, five White Cockatoo parrots (Cacatua alba) with clinical signs of PBFD were examined. After euthanasia, a full necropsy of parrots was performed and organs with macroscopic changes were sampled for routine histopathological evaluation. To confirm the presence of psittacine beak and feather disease virus (PBFDv), feather samples were analyzed with the PCR method. Sequence analysis of the obtained PCR products indicated their close relationship (99%) to other PBFDv isolates. Six variable nucleotide sites were discovered, two missense and four silent mutations. This paper presents the evidence of new PBFDv sequence in Cockatoo species.
Keywords:
PBFD / PCR diagnostic / nucleotide sequence / Cacatua albaSource:
Journal of The Hellenic Veterinary Medical Society, 2017, 68, 4, 653-660Publisher:
- Hellenic Veterinary Medical Soc, Athens
Funding / projects:
- Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
DOI: 10.12681/jhvms.16070
ISSN: 1792-2720
WoS: 000431139300022
Scopus: 2-s2.0-85043455810
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Fakultet veterinarske medicineTY - JOUR AU - Vučićević, Miloš AU - Vučićević, Ivana AU - Davitkov, Darko AU - Davitkov, Dajana AU - Stevanović, Jevrosima AU - Resanović, Radmila AU - Stanimirović, Zoran PY - 2017 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1433 AB - Psittacine beak and feather disease (PBFD) affects a large number of Psittaciformes species. In this study, five White Cockatoo parrots (Cacatua alba) with clinical signs of PBFD were examined. After euthanasia, a full necropsy of parrots was performed and organs with macroscopic changes were sampled for routine histopathological evaluation. To confirm the presence of psittacine beak and feather disease virus (PBFDv), feather samples were analyzed with the PCR method. Sequence analysis of the obtained PCR products indicated their close relationship (99%) to other PBFDv isolates. Six variable nucleotide sites were discovered, two missense and four silent mutations. This paper presents the evidence of new PBFDv sequence in Cockatoo species. PB - Hellenic Veterinary Medical Soc, Athens T2 - Journal of The Hellenic Veterinary Medical Society T1 - Detection and analysis of new psittacine beak and feather disease virus (PBFDv) nucleotide sequences VL - 68 IS - 4 SP - 653 EP - 660 DO - 10.12681/jhvms.16070 ER -
@article{ author = "Vučićević, Miloš and Vučićević, Ivana and Davitkov, Darko and Davitkov, Dajana and Stevanović, Jevrosima and Resanović, Radmila and Stanimirović, Zoran", year = "2017", abstract = "Psittacine beak and feather disease (PBFD) affects a large number of Psittaciformes species. In this study, five White Cockatoo parrots (Cacatua alba) with clinical signs of PBFD were examined. After euthanasia, a full necropsy of parrots was performed and organs with macroscopic changes were sampled for routine histopathological evaluation. To confirm the presence of psittacine beak and feather disease virus (PBFDv), feather samples were analyzed with the PCR method. Sequence analysis of the obtained PCR products indicated their close relationship (99%) to other PBFDv isolates. Six variable nucleotide sites were discovered, two missense and four silent mutations. This paper presents the evidence of new PBFDv sequence in Cockatoo species.", publisher = "Hellenic Veterinary Medical Soc, Athens", journal = "Journal of The Hellenic Veterinary Medical Society", title = "Detection and analysis of new psittacine beak and feather disease virus (PBFDv) nucleotide sequences", volume = "68", number = "4", pages = "653-660", doi = "10.12681/jhvms.16070" }
Vučićević, M., Vučićević, I., Davitkov, D., Davitkov, D., Stevanović, J., Resanović, R.,& Stanimirović, Z.. (2017). Detection and analysis of new psittacine beak and feather disease virus (PBFDv) nucleotide sequences. in Journal of The Hellenic Veterinary Medical Society Hellenic Veterinary Medical Soc, Athens., 68(4), 653-660. https://doi.org/10.12681/jhvms.16070
Vučićević M, Vučićević I, Davitkov D, Davitkov D, Stevanović J, Resanović R, Stanimirović Z. Detection and analysis of new psittacine beak and feather disease virus (PBFDv) nucleotide sequences. in Journal of The Hellenic Veterinary Medical Society. 2017;68(4):653-660. doi:10.12681/jhvms.16070 .
Vučićević, Miloš, Vučićević, Ivana, Davitkov, Darko, Davitkov, Dajana, Stevanović, Jevrosima, Resanović, Radmila, Stanimirović, Zoran, "Detection and analysis of new psittacine beak and feather disease virus (PBFDv) nucleotide sequences" in Journal of The Hellenic Veterinary Medical Society, 68, no. 4 (2017):653-660, https://doi.org/10.12681/jhvms.16070 . .