Analysis of reproductive parameters after intrauterine insemination of sows with semen stored for different time periods
Analiza reproduktivnih parametara intrauterino osemenjenih krmača u zavisnosti od vremena čuvanja razređenog semena
2017
Преузимање 🢃
Аутори
Vakanjac, SlobodankaMagaš, Vladimir
Babić, Vladan
Maletić, Milan
Đurić, Spomenka
Apić, Jelena
Mirilović, Milorad
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Introduction. Artificial insemination of sows with cooled semen has become a routine practice in the swine industry and has almost completely ruled out natural mating. The storage time of the cooled semen and preservation of its qualitative parameters are of the upmost importance and depend partly on the semen extender used. The aim of this study was to determine the quality of cooled semen during storage in a thermobox with a commercial extender by assessment of sperm motility, cytomorphology, and assessment of reproductive parameters in sows after intrauterine insemination. Materials and Methods. The semen was preserved with Duragen® (Magapor, Spain) extender and stored at 17±1°C. A total of 110 sows were included in the study and randomly divided into four groups: K1 (n=25) , K2 (n=25), K3 (n=30), and K4 (n=30). The sows were inseminated twice with semen (>1x107/ml spermatozoa) which had been stored for 1 day (S1), 3 days (S3), 5 days (S5), or 7 days (S7). Pregnancy was diagnosed by... imaging ultrasound. Results and Conclusions. The best results were obtained when the sows were inseminated with semen stored for one day. However, there were no statistically significant differences in the number and vitality of newborn piglets when semen stored for 3 or 5 days was used. Intrauterine insemination resulted in a satisfactory number of farrowing's and piglets despite the fact that a relatively low number of spermatozoa was determined in each dose used. The use of semen with a higher dilution rate and its intrauterine application ensured a larger number of obtained doses from one ejaculate and indicates this would lead to a more profitable use of boars.
Uvod. Veštačko osemenjavanje svinja rashlađenim semenom je postala rutinska praksa u industrijskoj proizvodnji svinja, pa je u razvijenim zemljama skoro potpuno zamenilo prirodno parenje. U zavisnosti od vrste upotrebljenog razređivača, dužina čuvanja rashlađenog semena i očuvanje njegovih kvalitativnih parametara je od izuzetne važnosti. Cilj istraživanja je analiza kvaliteta rashlađenog semena tokom njegovog skladištenja u termoboksu pri 17±1°C. Materijal i metode. Seme je konzervirano upotrebom razređivača Duragen® (Magapor, Španija) i skladišteno pri temperaturi od 17±1°C. U ogled je uključeno 110 krmača, podeljenih u četiri grupe (K1, K3, K5, K7). Krmače su osemenjivane dvokratno semenom skladištenim 1 dan (S1), 3 dana (S3), 5 dana (S5) i 7 dana (S7). Ultrazvučnim pregledom je vršena dijagnostika graviditeta. Kvalitet semena je ocenjivan ispitivanjem pokretljivosti spermatozoida i citomorfološkim pregledom i praćenjem reproduktivnih parametara kod intrauterino osemenjenih krmača. ...Rezultati i zaključak. Najbolji rezultat je postignut osemenjavanjem krmača semenom starim jedan dan. Međutim, nije bilo značajne razlike u broju i vitalnosti oprašene prasadi korišćenjem rashlađenog semena starog 3 i 5 dana. Intrauterinim osemenjavanjem su dobijeni zadovoljavajući rezultati (procenat prašenja i veličina legla) sa manjim brojem spermatozoida u inseminacionoj dozi. Upotrebom semena sa većim razređenjem i njegovim intrauterinim deponovanjem, omogućeno je dobijanje većeg broja inseminacionih doza semena od jednog ejakulata i rentabilnije korišćenje nerastova u reprodukciji.
Кључне речи:
boar / cooled semen / intrauterine insemination / piglets / nerast / rashlađeno seme / intrauterino osemenjavanje / prasadИзвор:
Veterinarski Glasnik, 2017, 71, 2, 98-106Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Финансирање / пројекти:
- Молекуларно-генетичка и екофизиолошка истраживања у заштити аутохтоних анималних генетичких ресурса, очувању добробити, здравља и репродукције гајених животиња и производњи безбедне хране (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Vakanjac, Slobodanka AU - Magaš, Vladimir AU - Babić, Vladan AU - Maletić, Milan AU - Đurić, Spomenka AU - Apić, Jelena AU - Mirilović, Milorad PY - 2017 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1444 AB - Introduction. Artificial insemination of sows with cooled semen has become a routine practice in the swine industry and has almost completely ruled out natural mating. The storage time of the cooled semen and preservation of its qualitative parameters are of the upmost importance and depend partly on the semen extender used. The aim of this study was to determine the quality of cooled semen during storage in a thermobox with a commercial extender by assessment of sperm motility, cytomorphology, and assessment of reproductive parameters in sows after intrauterine insemination. Materials and Methods. The semen was preserved with Duragen® (Magapor, Spain) extender and stored at 17±1°C. A total of 110 sows were included in the study and randomly divided into four groups: K1 (n=25) , K2 (n=25), K3 (n=30), and K4 (n=30). The sows were inseminated twice with semen (>1x107/ml spermatozoa) which had been stored for 1 day (S1), 3 days (S3), 5 days (S5), or 7 days (S7). Pregnancy was diagnosed by imaging ultrasound. Results and Conclusions. The best results were obtained when the sows were inseminated with semen stored for one day. However, there were no statistically significant differences in the number and vitality of newborn piglets when semen stored for 3 or 5 days was used. Intrauterine insemination resulted in a satisfactory number of farrowing's and piglets despite the fact that a relatively low number of spermatozoa was determined in each dose used. The use of semen with a higher dilution rate and its intrauterine application ensured a larger number of obtained doses from one ejaculate and indicates this would lead to a more profitable use of boars. AB - Uvod. Veštačko osemenjavanje svinja rashlađenim semenom je postala rutinska praksa u industrijskoj proizvodnji svinja, pa je u razvijenim zemljama skoro potpuno zamenilo prirodno parenje. U zavisnosti od vrste upotrebljenog razređivača, dužina čuvanja rashlađenog semena i očuvanje njegovih kvalitativnih parametara je od izuzetne važnosti. Cilj istraživanja je analiza kvaliteta rashlađenog semena tokom njegovog skladištenja u termoboksu pri 17±1°C. Materijal i metode. Seme je konzervirano upotrebom razređivača Duragen® (Magapor, Španija) i skladišteno pri temperaturi od 17±1°C. U ogled je uključeno 110 krmača, podeljenih u četiri grupe (K1, K3, K5, K7). Krmače su osemenjivane dvokratno semenom skladištenim 1 dan (S1), 3 dana (S3), 5 dana (S5) i 7 dana (S7). Ultrazvučnim pregledom je vršena dijagnostika graviditeta. Kvalitet semena je ocenjivan ispitivanjem pokretljivosti spermatozoida i citomorfološkim pregledom i praćenjem reproduktivnih parametara kod intrauterino osemenjenih krmača. Rezultati i zaključak. Najbolji rezultat je postignut osemenjavanjem krmača semenom starim jedan dan. Međutim, nije bilo značajne razlike u broju i vitalnosti oprašene prasadi korišćenjem rashlađenog semena starog 3 i 5 dana. Intrauterinim osemenjavanjem su dobijeni zadovoljavajući rezultati (procenat prašenja i veličina legla) sa manjim brojem spermatozoida u inseminacionoj dozi. Upotrebom semena sa većim razređenjem i njegovim intrauterinim deponovanjem, omogućeno je dobijanje većeg broja inseminacionih doza semena od jednog ejakulata i rentabilnije korišćenje nerastova u reprodukciji. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Veterinarski Glasnik T1 - Analysis of reproductive parameters after intrauterine insemination of sows with semen stored for different time periods T1 - Analiza reproduktivnih parametara intrauterino osemenjenih krmača u zavisnosti od vremena čuvanja razređenog semena VL - 71 IS - 2 SP - 98 EP - 106 DO - 10.2298/VETGL1702098V ER -
@article{ author = "Vakanjac, Slobodanka and Magaš, Vladimir and Babić, Vladan and Maletić, Milan and Đurić, Spomenka and Apić, Jelena and Mirilović, Milorad", year = "2017", abstract = "Introduction. Artificial insemination of sows with cooled semen has become a routine practice in the swine industry and has almost completely ruled out natural mating. The storage time of the cooled semen and preservation of its qualitative parameters are of the upmost importance and depend partly on the semen extender used. The aim of this study was to determine the quality of cooled semen during storage in a thermobox with a commercial extender by assessment of sperm motility, cytomorphology, and assessment of reproductive parameters in sows after intrauterine insemination. Materials and Methods. The semen was preserved with Duragen® (Magapor, Spain) extender and stored at 17±1°C. A total of 110 sows were included in the study and randomly divided into four groups: K1 (n=25) , K2 (n=25), K3 (n=30), and K4 (n=30). The sows were inseminated twice with semen (>1x107/ml spermatozoa) which had been stored for 1 day (S1), 3 days (S3), 5 days (S5), or 7 days (S7). Pregnancy was diagnosed by imaging ultrasound. Results and Conclusions. The best results were obtained when the sows were inseminated with semen stored for one day. However, there were no statistically significant differences in the number and vitality of newborn piglets when semen stored for 3 or 5 days was used. Intrauterine insemination resulted in a satisfactory number of farrowing's and piglets despite the fact that a relatively low number of spermatozoa was determined in each dose used. The use of semen with a higher dilution rate and its intrauterine application ensured a larger number of obtained doses from one ejaculate and indicates this would lead to a more profitable use of boars., Uvod. Veštačko osemenjavanje svinja rashlađenim semenom je postala rutinska praksa u industrijskoj proizvodnji svinja, pa je u razvijenim zemljama skoro potpuno zamenilo prirodno parenje. U zavisnosti od vrste upotrebljenog razređivača, dužina čuvanja rashlađenog semena i očuvanje njegovih kvalitativnih parametara je od izuzetne važnosti. Cilj istraživanja je analiza kvaliteta rashlađenog semena tokom njegovog skladištenja u termoboksu pri 17±1°C. Materijal i metode. Seme je konzervirano upotrebom razređivača Duragen® (Magapor, Španija) i skladišteno pri temperaturi od 17±1°C. U ogled je uključeno 110 krmača, podeljenih u četiri grupe (K1, K3, K5, K7). Krmače su osemenjivane dvokratno semenom skladištenim 1 dan (S1), 3 dana (S3), 5 dana (S5) i 7 dana (S7). Ultrazvučnim pregledom je vršena dijagnostika graviditeta. Kvalitet semena je ocenjivan ispitivanjem pokretljivosti spermatozoida i citomorfološkim pregledom i praćenjem reproduktivnih parametara kod intrauterino osemenjenih krmača. Rezultati i zaključak. Najbolji rezultat je postignut osemenjavanjem krmača semenom starim jedan dan. Međutim, nije bilo značajne razlike u broju i vitalnosti oprašene prasadi korišćenjem rashlađenog semena starog 3 i 5 dana. Intrauterinim osemenjavanjem su dobijeni zadovoljavajući rezultati (procenat prašenja i veličina legla) sa manjim brojem spermatozoida u inseminacionoj dozi. Upotrebom semena sa većim razređenjem i njegovim intrauterinim deponovanjem, omogućeno je dobijanje većeg broja inseminacionih doza semena od jednog ejakulata i rentabilnije korišćenje nerastova u reprodukciji.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Veterinarski Glasnik", title = "Analysis of reproductive parameters after intrauterine insemination of sows with semen stored for different time periods, Analiza reproduktivnih parametara intrauterino osemenjenih krmača u zavisnosti od vremena čuvanja razređenog semena", volume = "71", number = "2", pages = "98-106", doi = "10.2298/VETGL1702098V" }
Vakanjac, S., Magaš, V., Babić, V., Maletić, M., Đurić, S., Apić, J.,& Mirilović, M.. (2017). Analysis of reproductive parameters after intrauterine insemination of sows with semen stored for different time periods. in Veterinarski Glasnik Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 71(2), 98-106. https://doi.org/10.2298/VETGL1702098V
Vakanjac S, Magaš V, Babić V, Maletić M, Đurić S, Apić J, Mirilović M. Analysis of reproductive parameters after intrauterine insemination of sows with semen stored for different time periods. in Veterinarski Glasnik. 2017;71(2):98-106. doi:10.2298/VETGL1702098V .
Vakanjac, Slobodanka, Magaš, Vladimir, Babić, Vladan, Maletić, Milan, Đurić, Spomenka, Apić, Jelena, Mirilović, Milorad, "Analysis of reproductive parameters after intrauterine insemination of sows with semen stored for different time periods" in Veterinarski Glasnik, 71, no. 2 (2017):98-106, https://doi.org/10.2298/VETGL1702098V . .