Evaluation of cytotoxic and genotoxic effects of permethrin using in vitro micronucleus test
Evaluacija citotoksičnih i genotoksičnih efekata permetrina upotrebom in vitro mikronukleus testa
Апстракт
The objectives of this investigation were to evaluate the effects of the synthetic pyrethroid insecticide permethrin on mitotic activity and appearance of micronuclei in cultured human lymphocytes. In the standard cytokinesis-blocked micronucleus test in vitro protocol, four experimental concentrations were used: 1.2, 3, 6 and 12 mg/ml. The concentration of 6 mg/ml was calculated to correspond to the LD50 dose obtained in rats. The cytokinesis block proliferation index (CBPI), as a parameter of cell cycle kinetics, was decreased at all concentrations of permethrin except the lowest one. The analysis of micronucleus frequencies, however, point to the absence of genotoxic effects.
Cilj ovih istraživanj bio je da se izvrši evaluacija efekata sintetičkog piretroidnog insekticida permetrina analizom mitotske aktivnosti i pojave mikronukleusa u kulturama humanih limfocita. U standardnom in vitro mikronukleus testu upotrebljene su četiri eksperimentalne koncentracije permetrina: 1.2, 3, 6 i 12 mg/ml. Koncentracija od 6 mg/ml proračunata je da odgovara LD50 dozi kod pacova. Vrednosti proliferacionog indeksa pri blokiranoj citokinezi (CBPI) kao pokazatelja ćelijske kinetike, smanjene su pri svim koncentracijama permetrina osim najniže. Analize frekvenci mikronukleusa, međutim, ukazuju na odsustvo genotoksičnih efekata.
Кључне речи:
permethrin / lymphocyte / cell proliferation / micronucleusИзвор:
Acta Veterinaria-Beograd, 2000, 50, 4, 263-270Издавач:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Đelić, Ninoslav AU - Đelić, Dijana PY - 2000 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/145 AB - The objectives of this investigation were to evaluate the effects of the synthetic pyrethroid insecticide permethrin on mitotic activity and appearance of micronuclei in cultured human lymphocytes. In the standard cytokinesis-blocked micronucleus test in vitro protocol, four experimental concentrations were used: 1.2, 3, 6 and 12 mg/ml. The concentration of 6 mg/ml was calculated to correspond to the LD50 dose obtained in rats. The cytokinesis block proliferation index (CBPI), as a parameter of cell cycle kinetics, was decreased at all concentrations of permethrin except the lowest one. The analysis of micronucleus frequencies, however, point to the absence of genotoxic effects. AB - Cilj ovih istraživanj bio je da se izvrši evaluacija efekata sintetičkog piretroidnog insekticida permetrina analizom mitotske aktivnosti i pojave mikronukleusa u kulturama humanih limfocita. U standardnom in vitro mikronukleus testu upotrebljene su četiri eksperimentalne koncentracije permetrina: 1.2, 3, 6 i 12 mg/ml. Koncentracija od 6 mg/ml proračunata je da odgovara LD50 dozi kod pacova. Vrednosti proliferacionog indeksa pri blokiranoj citokinezi (CBPI) kao pokazatelja ćelijske kinetike, smanjene su pri svim koncentracijama permetrina osim najniže. Analize frekvenci mikronukleusa, međutim, ukazuju na odsustvo genotoksičnih efekata. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Evaluation of cytotoxic and genotoxic effects of permethrin using in vitro micronucleus test T1 - Evaluacija citotoksičnih i genotoksičnih efekata permetrina upotrebom in vitro mikronukleus testa VL - 50 IS - 4 SP - 263 EP - 270 UR - https://hdl.handle.net/21.15107/rcub_veterinar_145 ER -
@article{ author = "Đelić, Ninoslav and Đelić, Dijana", year = "2000", abstract = "The objectives of this investigation were to evaluate the effects of the synthetic pyrethroid insecticide permethrin on mitotic activity and appearance of micronuclei in cultured human lymphocytes. In the standard cytokinesis-blocked micronucleus test in vitro protocol, four experimental concentrations were used: 1.2, 3, 6 and 12 mg/ml. The concentration of 6 mg/ml was calculated to correspond to the LD50 dose obtained in rats. The cytokinesis block proliferation index (CBPI), as a parameter of cell cycle kinetics, was decreased at all concentrations of permethrin except the lowest one. The analysis of micronucleus frequencies, however, point to the absence of genotoxic effects., Cilj ovih istraživanj bio je da se izvrši evaluacija efekata sintetičkog piretroidnog insekticida permetrina analizom mitotske aktivnosti i pojave mikronukleusa u kulturama humanih limfocita. U standardnom in vitro mikronukleus testu upotrebljene su četiri eksperimentalne koncentracije permetrina: 1.2, 3, 6 i 12 mg/ml. Koncentracija od 6 mg/ml proračunata je da odgovara LD50 dozi kod pacova. Vrednosti proliferacionog indeksa pri blokiranoj citokinezi (CBPI) kao pokazatelja ćelijske kinetike, smanjene su pri svim koncentracijama permetrina osim najniže. Analize frekvenci mikronukleusa, međutim, ukazuju na odsustvo genotoksičnih efekata.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Evaluation of cytotoxic and genotoxic effects of permethrin using in vitro micronucleus test, Evaluacija citotoksičnih i genotoksičnih efekata permetrina upotrebom in vitro mikronukleus testa", volume = "50", number = "4", pages = "263-270", url = "https://hdl.handle.net/21.15107/rcub_veterinar_145" }
Đelić, N.,& Đelić, D.. (2000). Evaluation of cytotoxic and genotoxic effects of permethrin using in vitro micronucleus test. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 50(4), 263-270. https://hdl.handle.net/21.15107/rcub_veterinar_145
Đelić N, Đelić D. Evaluation of cytotoxic and genotoxic effects of permethrin using in vitro micronucleus test. in Acta Veterinaria-Beograd. 2000;50(4):263-270. https://hdl.handle.net/21.15107/rcub_veterinar_145 .
Đelić, Ninoslav, Đelić, Dijana, "Evaluation of cytotoxic and genotoxic effects of permethrin using in vitro micronucleus test" in Acta Veterinaria-Beograd, 50, no. 4 (2000):263-270, https://hdl.handle.net/21.15107/rcub_veterinar_145 .