Evaluation of cytotoxic and genotoxic effects of permethrin using in vitro micronucleus test

Abstract

The objectives of this investigation were to evaluate the effects of the synthetic pyrethroid insecticide permethrin on mitotic activity and appearance of micronuclei in cultured human lymphocytes. In the standard cytokinesis-blocked micronucleus test in vitro protocol, four experimental concentrations were used: 1.2, 3, 6 and 12 mg/ml. The concentration of 6 mg/ml was calculated to correspond to the LD50 dose obtained in rats. The cytokinesis block proliferation index (CBPI), as a parameter of cell cycle kinetics, was decreased at all concentrations of permethrin except the lowest one. The analysis of micronucleus frequencies, however, point to the absence of genotoxic effects.

Cilj ovih istraživanj bio je da se izvrši evaluacija efekata sintetičkog piretroidnog insekticida permetrina analizom mitotske aktivnosti i pojave mikronukleusa u kulturama humanih limfocita. U standardnom in vitro mikronukleus testu upotrebljene su četiri eksperimentalne koncentracije permetrina: 1.2, 3, 6 i 12 mg/ml. Koncentracija od 6 mg/ml proračunata je da odgovara LD50 dozi kod pacova. Vrednosti proliferacionog indeksa pri blokiranoj citokinezi (CBPI) kao pokazatelja ćelijske kinetike, smanjene su pri svim koncentracijama permetrina osim najniže. Analize frekvenci mikronukleusa, međutim, ukazuju na odsustvo genotoksičnih efekata.