Molecular identification of Prototheca zopfii genotype 2 mastitis isolates and their influence on the milk somatic cell count [Molekularna identifikacija izolata Prototheca zopfi i genotip 2 izdvojenih kod mastitisa krava i njihov utjecaj na broj somatskih stanica u mlijeku
2017
Autori
Suvajdžić, BrankoVasilev, Dragan
Karabasil, Nedjeljko
Vucurović, Ivan
Čobanović, Nikola
Babić, Milijana
Katić, Vera
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Algae from the genus Prototheca are the only plant-like microorganisms which can cause infl ammation and alterations in the mammary gland. Prototheca mastitis is usually recognized as a chronic and symptomless disease with reduced milk production and a very high somatic cell count. Molecular identifi cation of Prototheca spp. is helpful for the differentiation of pathogenic from non-pathogenic strains which are probably milk contaminants. Genotype-specifi c PCR assays, based on the 18S rDNA gene sequences, have recently been developed to differentiate three genotypes of Prototheca zopfi i, of which Prototheca zopfi i genotype 3 was reclassifi ed in a new species: Prototheca blaschkeae. P. zopfi i genotype 2 is characterized as the main causative agent of Prototheca mastitis that leads to signifi cant economic losses in primary milk production. The purpose of this study was to give a molecular characterization of Prototheca strains isolated in cases of subclinical and clinical mastitis,... as well as to determine the infl uence of these pathogenic algae on the milk somatic cell count. After microbiological examination, algae from the genus Prototheca were isolated in pure cultures from 1.8% of all tested milk samples, and all 13 (100%) isolates were determined as Prototheca zopfi i genotype 2 by a genotype-specifi c PCR. This study has provided the fi rst molecular identifi cation of Prototheca zopfi i genotype 2 in the Republic of Serbia. In the case of subclinical Prototheca mastitis, the somatic cell count was 4,175,244 ± 1,233,685/mL of milk. A distinctly higher somatic cell count (P lt 0.05) was found in the quarters infected by Prototheca zopfi i genotype 2 than in the quarters infected by Staphylococcus aureus, which is the most common mastitis causative agent worldwide. The results from this study support previous observations that P. zopfi i genotype 2 is the main causative agent of Prototheca mastitis which leads to a signifi cant increase in the somatic cell count in the milk.
Ključne reči:
Prototheca mastitis / Prototheca zopfii genotype 2 / genotype-specific PCR / somatic cell countIzvor:
Veterinarski Arhiv, 2017, 87, 3, 249-258Izdavač:
- Univ Zagreb Vet Faculty, Zagreb
Finansiranje / projekti:
DOI: 10.24099/vet.arhiv.151219
ISSN: 0372-5480
WoS: 000403577000002
Scopus: 2-s2.0-85019154220
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Suvajdžić, Branko AU - Vasilev, Dragan AU - Karabasil, Nedjeljko AU - Vucurović, Ivan AU - Čobanović, Nikola AU - Babić, Milijana AU - Katić, Vera PY - 2017 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1467 AB - Algae from the genus Prototheca are the only plant-like microorganisms which can cause infl ammation and alterations in the mammary gland. Prototheca mastitis is usually recognized as a chronic and symptomless disease with reduced milk production and a very high somatic cell count. Molecular identifi cation of Prototheca spp. is helpful for the differentiation of pathogenic from non-pathogenic strains which are probably milk contaminants. Genotype-specifi c PCR assays, based on the 18S rDNA gene sequences, have recently been developed to differentiate three genotypes of Prototheca zopfi i, of which Prototheca zopfi i genotype 3 was reclassifi ed in a new species: Prototheca blaschkeae. P. zopfi i genotype 2 is characterized as the main causative agent of Prototheca mastitis that leads to signifi cant economic losses in primary milk production. The purpose of this study was to give a molecular characterization of Prototheca strains isolated in cases of subclinical and clinical mastitis, as well as to determine the infl uence of these pathogenic algae on the milk somatic cell count. After microbiological examination, algae from the genus Prototheca were isolated in pure cultures from 1.8% of all tested milk samples, and all 13 (100%) isolates were determined as Prototheca zopfi i genotype 2 by a genotype-specifi c PCR. This study has provided the fi rst molecular identifi cation of Prototheca zopfi i genotype 2 in the Republic of Serbia. In the case of subclinical Prototheca mastitis, the somatic cell count was 4,175,244 ± 1,233,685/mL of milk. A distinctly higher somatic cell count (P lt 0.05) was found in the quarters infected by Prototheca zopfi i genotype 2 than in the quarters infected by Staphylococcus aureus, which is the most common mastitis causative agent worldwide. The results from this study support previous observations that P. zopfi i genotype 2 is the main causative agent of Prototheca mastitis which leads to a signifi cant increase in the somatic cell count in the milk. PB - Univ Zagreb Vet Faculty, Zagreb T2 - Veterinarski Arhiv T1 - Molecular identification of Prototheca zopfii genotype 2 mastitis isolates and their influence on the milk somatic cell count [Molekularna identifikacija izolata Prototheca zopfi i genotip 2 izdvojenih kod mastitisa krava i njihov utjecaj na broj somatskih stanica u mlijeku VL - 87 IS - 3 SP - 249 EP - 258 DO - 10.24099/vet.arhiv.151219 ER -
@article{ author = "Suvajdžić, Branko and Vasilev, Dragan and Karabasil, Nedjeljko and Vucurović, Ivan and Čobanović, Nikola and Babić, Milijana and Katić, Vera", year = "2017", abstract = "Algae from the genus Prototheca are the only plant-like microorganisms which can cause infl ammation and alterations in the mammary gland. Prototheca mastitis is usually recognized as a chronic and symptomless disease with reduced milk production and a very high somatic cell count. Molecular identifi cation of Prototheca spp. is helpful for the differentiation of pathogenic from non-pathogenic strains which are probably milk contaminants. Genotype-specifi c PCR assays, based on the 18S rDNA gene sequences, have recently been developed to differentiate three genotypes of Prototheca zopfi i, of which Prototheca zopfi i genotype 3 was reclassifi ed in a new species: Prototheca blaschkeae. P. zopfi i genotype 2 is characterized as the main causative agent of Prototheca mastitis that leads to signifi cant economic losses in primary milk production. The purpose of this study was to give a molecular characterization of Prototheca strains isolated in cases of subclinical and clinical mastitis, as well as to determine the infl uence of these pathogenic algae on the milk somatic cell count. After microbiological examination, algae from the genus Prototheca were isolated in pure cultures from 1.8% of all tested milk samples, and all 13 (100%) isolates were determined as Prototheca zopfi i genotype 2 by a genotype-specifi c PCR. This study has provided the fi rst molecular identifi cation of Prototheca zopfi i genotype 2 in the Republic of Serbia. In the case of subclinical Prototheca mastitis, the somatic cell count was 4,175,244 ± 1,233,685/mL of milk. A distinctly higher somatic cell count (P lt 0.05) was found in the quarters infected by Prototheca zopfi i genotype 2 than in the quarters infected by Staphylococcus aureus, which is the most common mastitis causative agent worldwide. The results from this study support previous observations that P. zopfi i genotype 2 is the main causative agent of Prototheca mastitis which leads to a signifi cant increase in the somatic cell count in the milk.", publisher = "Univ Zagreb Vet Faculty, Zagreb", journal = "Veterinarski Arhiv", title = "Molecular identification of Prototheca zopfii genotype 2 mastitis isolates and their influence on the milk somatic cell count [Molekularna identifikacija izolata Prototheca zopfi i genotip 2 izdvojenih kod mastitisa krava i njihov utjecaj na broj somatskih stanica u mlijeku", volume = "87", number = "3", pages = "249-258", doi = "10.24099/vet.arhiv.151219" }
Suvajdžić, B., Vasilev, D., Karabasil, N., Vucurović, I., Čobanović, N., Babić, M.,& Katić, V.. (2017). Molecular identification of Prototheca zopfii genotype 2 mastitis isolates and their influence on the milk somatic cell count [Molekularna identifikacija izolata Prototheca zopfi i genotip 2 izdvojenih kod mastitisa krava i njihov utjecaj na broj somatskih stanica u mlijeku. in Veterinarski Arhiv Univ Zagreb Vet Faculty, Zagreb., 87(3), 249-258. https://doi.org/10.24099/vet.arhiv.151219
Suvajdžić B, Vasilev D, Karabasil N, Vucurović I, Čobanović N, Babić M, Katić V. Molecular identification of Prototheca zopfii genotype 2 mastitis isolates and their influence on the milk somatic cell count [Molekularna identifikacija izolata Prototheca zopfi i genotip 2 izdvojenih kod mastitisa krava i njihov utjecaj na broj somatskih stanica u mlijeku. in Veterinarski Arhiv. 2017;87(3):249-258. doi:10.24099/vet.arhiv.151219 .
Suvajdžić, Branko, Vasilev, Dragan, Karabasil, Nedjeljko, Vucurović, Ivan, Čobanović, Nikola, Babić, Milijana, Katić, Vera, "Molecular identification of Prototheca zopfii genotype 2 mastitis isolates and their influence on the milk somatic cell count [Molekularna identifikacija izolata Prototheca zopfi i genotip 2 izdvojenih kod mastitisa krava i njihov utjecaj na broj somatskih stanica u mlijeku" in Veterinarski Arhiv, 87, no. 3 (2017):249-258, https://doi.org/10.24099/vet.arhiv.151219 . .