Assessment of 17 microsatellite loci for their use in parentage verification and individual identification in the Balkan donkey breed
2017
Преузимање 🢃
Аутори
Stanišić, LjubodragDimitrijević, Vladimir
Simeunović, Predrag
Glavinić, Uroš
Jovanović, Biljana
Stevanović, Jevrosima
Stanimirović, Zoran
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of this study was to assess a panel of 17 microsatellites for parentage verification and individual identification in the endangered Balkan donkey breed. Allele frequencies for 17 microsatellite loci (AHT4, AHT5, ASB2, ASB17, ASB23, CA425, HTG4, HTG6, HTG7, HTG10, HMS1, HMS2, HMS3, HMS6, HMS7, LEX3 and VHL20) were determined in a 77 unrelated Balkan donkeys. Three loci (ASB2, HMS1 and ASB17) proved to be unsuitable and had been excluded from the investigation. Analysis of the remaining 14 loci revealed varied levels of polymorphism (three to 12 alleles), while the total number of observed alleles was 118 with an average of 8.42 per locus. Average values of observed heterozygosity and polymorphic information content (PIC) were 0.712 and 0.650, respectively. Twelve out of 14 microsatellite markers were highly informative with PIC values higher than 0.5. Only four loci were in HWE (HMS2, HMS6, HMS7 and HTG6). The obtained value of combined power of exclusion 0.9999) confirms usefu...lness of this microsatellite panel for parentage verification, while the value of combined power of discrimination of 0.9941 clearly approves the reliability of the panel for individual identification in Balkan donkeys.
Кључне речи:
allele frequency / Balkan donkey / equine microsatellites / parentageИзвор:
Genetika, 2017, 49, 1, 21-30Издавач:
- Društvo genetičara Srbije, Beograd
Финансирање / пројекти:
- Молекуларно-генетичка и екофизиолошка истраживања у заштити аутохтоних анималних генетичких ресурса, очувању добробити, здравља и репродукције гајених животиња и производњи безбедне хране (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
DOI: 10.2298/GENSR1701021S
ISSN: 0534-0012
WoS: 000405684200002
Scopus: 2-s2.0-85026224551
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Stanišić, Ljubodrag AU - Dimitrijević, Vladimir AU - Simeunović, Predrag AU - Glavinić, Uroš AU - Jovanović, Biljana AU - Stevanović, Jevrosima AU - Stanimirović, Zoran PY - 2017 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1474 AB - The aim of this study was to assess a panel of 17 microsatellites for parentage verification and individual identification in the endangered Balkan donkey breed. Allele frequencies for 17 microsatellite loci (AHT4, AHT5, ASB2, ASB17, ASB23, CA425, HTG4, HTG6, HTG7, HTG10, HMS1, HMS2, HMS3, HMS6, HMS7, LEX3 and VHL20) were determined in a 77 unrelated Balkan donkeys. Three loci (ASB2, HMS1 and ASB17) proved to be unsuitable and had been excluded from the investigation. Analysis of the remaining 14 loci revealed varied levels of polymorphism (three to 12 alleles), while the total number of observed alleles was 118 with an average of 8.42 per locus. Average values of observed heterozygosity and polymorphic information content (PIC) were 0.712 and 0.650, respectively. Twelve out of 14 microsatellite markers were highly informative with PIC values higher than 0.5. Only four loci were in HWE (HMS2, HMS6, HMS7 and HTG6). The obtained value of combined power of exclusion 0.9999) confirms usefulness of this microsatellite panel for parentage verification, while the value of combined power of discrimination of 0.9941 clearly approves the reliability of the panel for individual identification in Balkan donkeys. PB - Društvo genetičara Srbije, Beograd T2 - Genetika T1 - Assessment of 17 microsatellite loci for their use in parentage verification and individual identification in the Balkan donkey breed VL - 49 IS - 1 SP - 21 EP - 30 DO - 10.2298/GENSR1701021S ER -
@article{ author = "Stanišić, Ljubodrag and Dimitrijević, Vladimir and Simeunović, Predrag and Glavinić, Uroš and Jovanović, Biljana and Stevanović, Jevrosima and Stanimirović, Zoran", year = "2017", abstract = "The aim of this study was to assess a panel of 17 microsatellites for parentage verification and individual identification in the endangered Balkan donkey breed. Allele frequencies for 17 microsatellite loci (AHT4, AHT5, ASB2, ASB17, ASB23, CA425, HTG4, HTG6, HTG7, HTG10, HMS1, HMS2, HMS3, HMS6, HMS7, LEX3 and VHL20) were determined in a 77 unrelated Balkan donkeys. Three loci (ASB2, HMS1 and ASB17) proved to be unsuitable and had been excluded from the investigation. Analysis of the remaining 14 loci revealed varied levels of polymorphism (three to 12 alleles), while the total number of observed alleles was 118 with an average of 8.42 per locus. Average values of observed heterozygosity and polymorphic information content (PIC) were 0.712 and 0.650, respectively. Twelve out of 14 microsatellite markers were highly informative with PIC values higher than 0.5. Only four loci were in HWE (HMS2, HMS6, HMS7 and HTG6). The obtained value of combined power of exclusion 0.9999) confirms usefulness of this microsatellite panel for parentage verification, while the value of combined power of discrimination of 0.9941 clearly approves the reliability of the panel for individual identification in Balkan donkeys.", publisher = "Društvo genetičara Srbije, Beograd", journal = "Genetika", title = "Assessment of 17 microsatellite loci for their use in parentage verification and individual identification in the Balkan donkey breed", volume = "49", number = "1", pages = "21-30", doi = "10.2298/GENSR1701021S" }
Stanišić, L., Dimitrijević, V., Simeunović, P., Glavinić, U., Jovanović, B., Stevanović, J.,& Stanimirović, Z.. (2017). Assessment of 17 microsatellite loci for their use in parentage verification and individual identification in the Balkan donkey breed. in Genetika Društvo genetičara Srbije, Beograd., 49(1), 21-30. https://doi.org/10.2298/GENSR1701021S
Stanišić L, Dimitrijević V, Simeunović P, Glavinić U, Jovanović B, Stevanović J, Stanimirović Z. Assessment of 17 microsatellite loci for their use in parentage verification and individual identification in the Balkan donkey breed. in Genetika. 2017;49(1):21-30. doi:10.2298/GENSR1701021S .
Stanišić, Ljubodrag, Dimitrijević, Vladimir, Simeunović, Predrag, Glavinić, Uroš, Jovanović, Biljana, Stevanović, Jevrosima, Stanimirović, Zoran, "Assessment of 17 microsatellite loci for their use in parentage verification and individual identification in the Balkan donkey breed" in Genetika, 49, no. 1 (2017):21-30, https://doi.org/10.2298/GENSR1701021S . .