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dc.creatorStanišić, Ljubodrag
dc.creatorDimitrijević, Vladimir
dc.creatorSimeunović, Predrag
dc.creatorGlavinić, Uroš
dc.creatorJovanović, Biljana
dc.creatorStevanović, Jevrosima
dc.creatorStanimirović, Zoran
dc.date.accessioned2020-06-03T14:14:39Z
dc.date.available2020-06-03T14:14:39Z
dc.date.issued2017
dc.identifier.issn0534-0012
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1474
dc.description.abstractThe aim of this study was to assess a panel of 17 microsatellites for parentage verification and individual identification in the endangered Balkan donkey breed. Allele frequencies for 17 microsatellite loci (AHT4, AHT5, ASB2, ASB17, ASB23, CA425, HTG4, HTG6, HTG7, HTG10, HMS1, HMS2, HMS3, HMS6, HMS7, LEX3 and VHL20) were determined in a 77 unrelated Balkan donkeys. Three loci (ASB2, HMS1 and ASB17) proved to be unsuitable and had been excluded from the investigation. Analysis of the remaining 14 loci revealed varied levels of polymorphism (three to 12 alleles), while the total number of observed alleles was 118 with an average of 8.42 per locus. Average values of observed heterozygosity and polymorphic information content (PIC) were 0.712 and 0.650, respectively. Twelve out of 14 microsatellite markers were highly informative with PIC values higher than 0.5. Only four loci were in HWE (HMS2, HMS6, HMS7 and HTG6). The obtained value of combined power of exclusion 0.9999) confirms usefulness of this microsatellite panel for parentage verification, while the value of combined power of discrimination of 0.9941 clearly approves the reliability of the panel for individual identification in Balkan donkeys.en
dc.publisherDruštvo genetičara Srbije, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46002/RS//
dc.rightsopenAccess
dc.sourceGenetika
dc.subjectallele frequencyen
dc.subjectBalkan donkeyen
dc.subjectequine microsatellitesen
dc.subjectparentageen
dc.titleAssessment of 17 microsatellite loci for their use in parentage verification and individual identification in the Balkan donkey breeden
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractГлавинић, Урош; Стевановић, Јевросима; Јовановић, Биљана; Димитријевић, Владимир; Симеуновић, Предраг; Станимировић, Зоран; Станишић, Љубодраг;
dc.citation.volume49
dc.citation.issue1
dc.citation.spage21
dc.citation.epage30
dc.citation.other49(1): 21-30
dc.citation.rankM23
dc.identifier.wos000405684200002
dc.identifier.doi10.2298/GENSR1701021S
dc.identifier.scopus2-s2.0-85026224551
dc.identifier.fulltexthttp://veterinar.vet.bg.ac.rs/bitstream/id/434/1473.pdf
dc.identifier.rcubconv_2272
dc.type.versionpublishedVersion


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