Detection of ICAA and ICAD genes of staphylococcus aureus isolated in cases of bovine mastitis in the republic of Serbia
2017
Authors
Suvajdžić, BrankoTeodorović, Vlado
Vasilev, Dragan
Karabasil, Nedjeljko
Dimitrijević, Mirjana
Đorđević, Jasna
Katić, Vera
Article (Published version)
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Show full item recordAbstract
Staphylococcus aureus (S.aureus) is the most common agent of contagious mastitis that causes serious health and economic problems. The ability to form biofilms is an important virulence factor of S.aureus for the establishment of persistent infections. This study is aimed to investigate the presence of icaA and icaD, two genes of importance for the biofilm formation in S. aureus bovine mastitis isolates. In order to isolate and identify S. aureus, 1555 milk samples were collected from 401 cows, located in different regions of the Republic of Serbia. Using the conventional microbiological methods 100 isolates were characterized as coagulase-positive staphylococci. After primary biochemical identification, the species confirmation of 44 S. aureus isolates was done using MALDI-TOF Mass Spectrometry and PCR technique, targeting the S. aureus-specific nuc gene. Among all investigated S. aureus isolates 25.0% harbored both icaA gene and icaD genes. The presence of icaD gene alone was confirm...ed in 40.9% of cases totaling icaD positive isolates to 65.9%. The remaining 34.1% of S. aureus isolates were negative for the presence of both genes. The results of the present study indicate the existence of potential biofilm-producer strains in different regions of the Republic of Serbia, both under intensive and semi-extensive cows breeding.
Keywords:
biofi lm / icaA gene / icaD gene / mastitis / Staphylococcus aureusSource:
Acta Veterinaria-Beograd, 2017, 67, 2, 168-177Publisher:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Funding / projects:
- Optimization of technological procedures and zootehnical resources on farms with the goal to upgrade sustainability of milk production (RS-MESTD-Technological Development (TD or TR)-31086)
DOI: 10.1515/acve-2017-0015
ISSN: 0567-8315
WoS: 000406311800003
Scopus: 2-s2.0-85021435152
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Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Suvajdžić, Branko AU - Teodorović, Vlado AU - Vasilev, Dragan AU - Karabasil, Nedjeljko AU - Dimitrijević, Mirjana AU - Đorđević, Jasna AU - Katić, Vera PY - 2017 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1476 AB - Staphylococcus aureus (S.aureus) is the most common agent of contagious mastitis that causes serious health and economic problems. The ability to form biofilms is an important virulence factor of S.aureus for the establishment of persistent infections. This study is aimed to investigate the presence of icaA and icaD, two genes of importance for the biofilm formation in S. aureus bovine mastitis isolates. In order to isolate and identify S. aureus, 1555 milk samples were collected from 401 cows, located in different regions of the Republic of Serbia. Using the conventional microbiological methods 100 isolates were characterized as coagulase-positive staphylococci. After primary biochemical identification, the species confirmation of 44 S. aureus isolates was done using MALDI-TOF Mass Spectrometry and PCR technique, targeting the S. aureus-specific nuc gene. Among all investigated S. aureus isolates 25.0% harbored both icaA gene and icaD genes. The presence of icaD gene alone was confirmed in 40.9% of cases totaling icaD positive isolates to 65.9%. The remaining 34.1% of S. aureus isolates were negative for the presence of both genes. The results of the present study indicate the existence of potential biofilm-producer strains in different regions of the Republic of Serbia, both under intensive and semi-extensive cows breeding. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Detection of ICAA and ICAD genes of staphylococcus aureus isolated in cases of bovine mastitis in the republic of Serbia VL - 67 IS - 2 SP - 168 EP - 177 DO - 10.1515/acve-2017-0015 ER -
@article{ author = "Suvajdžić, Branko and Teodorović, Vlado and Vasilev, Dragan and Karabasil, Nedjeljko and Dimitrijević, Mirjana and Đorđević, Jasna and Katić, Vera", year = "2017", abstract = "Staphylococcus aureus (S.aureus) is the most common agent of contagious mastitis that causes serious health and economic problems. The ability to form biofilms is an important virulence factor of S.aureus for the establishment of persistent infections. This study is aimed to investigate the presence of icaA and icaD, two genes of importance for the biofilm formation in S. aureus bovine mastitis isolates. In order to isolate and identify S. aureus, 1555 milk samples were collected from 401 cows, located in different regions of the Republic of Serbia. Using the conventional microbiological methods 100 isolates were characterized as coagulase-positive staphylococci. After primary biochemical identification, the species confirmation of 44 S. aureus isolates was done using MALDI-TOF Mass Spectrometry and PCR technique, targeting the S. aureus-specific nuc gene. Among all investigated S. aureus isolates 25.0% harbored both icaA gene and icaD genes. The presence of icaD gene alone was confirmed in 40.9% of cases totaling icaD positive isolates to 65.9%. The remaining 34.1% of S. aureus isolates were negative for the presence of both genes. The results of the present study indicate the existence of potential biofilm-producer strains in different regions of the Republic of Serbia, both under intensive and semi-extensive cows breeding.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Detection of ICAA and ICAD genes of staphylococcus aureus isolated in cases of bovine mastitis in the republic of Serbia", volume = "67", number = "2", pages = "168-177", doi = "10.1515/acve-2017-0015" }
Suvajdžić, B., Teodorović, V., Vasilev, D., Karabasil, N., Dimitrijević, M., Đorđević, J.,& Katić, V.. (2017). Detection of ICAA and ICAD genes of staphylococcus aureus isolated in cases of bovine mastitis in the republic of Serbia. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(2), 168-177. https://doi.org/10.1515/acve-2017-0015
Suvajdžić B, Teodorović V, Vasilev D, Karabasil N, Dimitrijević M, Đorđević J, Katić V. Detection of ICAA and ICAD genes of staphylococcus aureus isolated in cases of bovine mastitis in the republic of Serbia. in Acta Veterinaria-Beograd. 2017;67(2):168-177. doi:10.1515/acve-2017-0015 .
Suvajdžić, Branko, Teodorović, Vlado, Vasilev, Dragan, Karabasil, Nedjeljko, Dimitrijević, Mirjana, Đorđević, Jasna, Katić, Vera, "Detection of ICAA and ICAD genes of staphylococcus aureus isolated in cases of bovine mastitis in the republic of Serbia" in Acta Veterinaria-Beograd, 67, no. 2 (2017):168-177, https://doi.org/10.1515/acve-2017-0015 . .