Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats
2017
Authors
Danilović-Luković, JelenaKorac, Aleksandra
Milošević, Ivan
Lužajić, Tijana
Milanović, Zorana
Kovačević-Filipović, Milica
Radovanović, Anita
Article (Published version)
Metadata
Show full item recordAbstract
Induced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle assembly and premature follicular atresia with ovary failure. This work investigates the influence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on the number and morphology of oogonia/oocytes in newborn rat pups with light and transmission electron microscopy. Expression of apoptosis and autophagy markers in oogonia/oocytes were examined using immunohistochemistry. Hypothyroid newborn pups had a decreased number of mitotic and resting oogonia, while the number of altered oogonia/oocytes was increased. Ultrastructural observations revealed the increased presence of degenerated pachytene oocytes (Z-cells) and oogonia/oocytes undergoing autophagy, apoptosis and combined apoptosis and autophagy, in this group. The most abundant altered oogonia/oocytes in the hypothyroid group were those with morphological features of advanced autophagy and Z-cells. The percentage of TUNEL (terminal... deoxynucleotidyl transferase dUTP nick end labeling) positive oogonia/ oocytes was significantly lower in the hypothyroid group. No significant difference was recorded in the expression of caspase-3, ATG7 and LC3 possibly reflecting that these proteins were not involved in the oogonia/ oocyte alteration process during prenatal rat hypothyroidism. The obtained results indicate that developmental hypothyroidism in the offspring enhances the number of Z-cells and oogonia/ oocytes altered with the advanced process of autophagy.
Keywords:
ATG7 / caspase-3 / LC3 / meiosis prophase I / secondary necrosisSource:
Acta Veterinaria-Beograd, 2017, 67, 1, 92-106Publisher:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Funding / projects:
- Antioxidative defense, differentiation and regeneration potential of tissue specific mesenchymal stem cells during ageing (RS-MESTD-Basic Research (BR or ON)-175061)
DOI: 10.1515/acve-2017-0009
ISSN: 0567-8315
WoS: 000401672900009
Scopus: 2-s2.0-85016747573
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Fakultet veterinarske medicineTY - JOUR AU - Danilović-Luković, Jelena AU - Korac, Aleksandra AU - Milošević, Ivan AU - Lužajić, Tijana AU - Milanović, Zorana AU - Kovačević-Filipović, Milica AU - Radovanović, Anita PY - 2017 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1477 AB - Induced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle assembly and premature follicular atresia with ovary failure. This work investigates the influence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on the number and morphology of oogonia/oocytes in newborn rat pups with light and transmission electron microscopy. Expression of apoptosis and autophagy markers in oogonia/oocytes were examined using immunohistochemistry. Hypothyroid newborn pups had a decreased number of mitotic and resting oogonia, while the number of altered oogonia/oocytes was increased. Ultrastructural observations revealed the increased presence of degenerated pachytene oocytes (Z-cells) and oogonia/oocytes undergoing autophagy, apoptosis and combined apoptosis and autophagy, in this group. The most abundant altered oogonia/oocytes in the hypothyroid group were those with morphological features of advanced autophagy and Z-cells. The percentage of TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) positive oogonia/ oocytes was significantly lower in the hypothyroid group. No significant difference was recorded in the expression of caspase-3, ATG7 and LC3 possibly reflecting that these proteins were not involved in the oogonia/ oocyte alteration process during prenatal rat hypothyroidism. The obtained results indicate that developmental hypothyroidism in the offspring enhances the number of Z-cells and oogonia/ oocytes altered with the advanced process of autophagy. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta Veterinaria-Beograd T1 - Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats VL - 67 IS - 1 SP - 92 EP - 106 DO - 10.1515/acve-2017-0009 ER -
@article{ author = "Danilović-Luković, Jelena and Korac, Aleksandra and Milošević, Ivan and Lužajić, Tijana and Milanović, Zorana and Kovačević-Filipović, Milica and Radovanović, Anita", year = "2017", abstract = "Induced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle assembly and premature follicular atresia with ovary failure. This work investigates the influence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on the number and morphology of oogonia/oocytes in newborn rat pups with light and transmission electron microscopy. Expression of apoptosis and autophagy markers in oogonia/oocytes were examined using immunohistochemistry. Hypothyroid newborn pups had a decreased number of mitotic and resting oogonia, while the number of altered oogonia/oocytes was increased. Ultrastructural observations revealed the increased presence of degenerated pachytene oocytes (Z-cells) and oogonia/oocytes undergoing autophagy, apoptosis and combined apoptosis and autophagy, in this group. The most abundant altered oogonia/oocytes in the hypothyroid group were those with morphological features of advanced autophagy and Z-cells. The percentage of TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) positive oogonia/ oocytes was significantly lower in the hypothyroid group. No significant difference was recorded in the expression of caspase-3, ATG7 and LC3 possibly reflecting that these proteins were not involved in the oogonia/ oocyte alteration process during prenatal rat hypothyroidism. The obtained results indicate that developmental hypothyroidism in the offspring enhances the number of Z-cells and oogonia/ oocytes altered with the advanced process of autophagy.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta Veterinaria-Beograd", title = "Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats", volume = "67", number = "1", pages = "92-106", doi = "10.1515/acve-2017-0009" }
Danilović-Luković, J., Korac, A., Milošević, I., Lužajić, T., Milanović, Z., Kovačević-Filipović, M.,& Radovanović, A.. (2017). Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats. in Acta Veterinaria-Beograd Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(1), 92-106. https://doi.org/10.1515/acve-2017-0009
Danilović-Luković J, Korac A, Milošević I, Lužajić T, Milanović Z, Kovačević-Filipović M, Radovanović A. Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats. in Acta Veterinaria-Beograd. 2017;67(1):92-106. doi:10.1515/acve-2017-0009 .
Danilović-Luković, Jelena, Korac, Aleksandra, Milošević, Ivan, Lužajić, Tijana, Milanović, Zorana, Kovačević-Filipović, Milica, Radovanović, Anita, "Z-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn rats" in Acta Veterinaria-Beograd, 67, no. 1 (2017):92-106, https://doi.org/10.1515/acve-2017-0009 . .