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dc.creatorDanilović-Luković, Jelena
dc.creatorKorac, Aleksandra
dc.creatorMilošević, Ivan
dc.creatorLužajić, Tijana
dc.creatorMilanović, Zorana
dc.creatorKovačević-Filipović, Milica
dc.creatorRadovanović, Anita
dc.date.accessioned2020-06-03T14:14:52Z
dc.date.available2020-06-03T14:14:52Z
dc.date.issued2017
dc.identifier.issn0567-8315
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1477
dc.description.abstractInduced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle assembly and premature follicular atresia with ovary failure. This work investigates the influence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on the number and morphology of oogonia/oocytes in newborn rat pups with light and transmission electron microscopy. Expression of apoptosis and autophagy markers in oogonia/oocytes were examined using immunohistochemistry. Hypothyroid newborn pups had a decreased number of mitotic and resting oogonia, while the number of altered oogonia/oocytes was increased. Ultrastructural observations revealed the increased presence of degenerated pachytene oocytes (Z-cells) and oogonia/oocytes undergoing autophagy, apoptosis and combined apoptosis and autophagy, in this group. The most abundant altered oogonia/oocytes in the hypothyroid group were those with morphological features of advanced autophagy and Z-cells. The percentage of TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) positive oogonia/ oocytes was significantly lower in the hypothyroid group. No significant difference was recorded in the expression of caspase-3, ATG7 and LC3 possibly reflecting that these proteins were not involved in the oogonia/ oocyte alteration process during prenatal rat hypothyroidism. The obtained results indicate that developmental hypothyroidism in the offspring enhances the number of Z-cells and oogonia/ oocytes altered with the advanced process of autophagy.en
dc.publisherUniverzitet u Beogradu - Fakultet veterinarske medicine, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175061/RS//
dc.rightsopenAccess
dc.sourceActa veterinaria - Beograd
dc.subjectATG7en
dc.subjectcaspase-3en
dc.subjectLC3en
dc.subjectmeiosis prophase Ien
dc.subjectsecondary necrosisen
dc.titleZ-cells and oogonia/oocytes in the advanced process of autophagy are the dominant altered cells in the ovaries of hypothyroid newborn ratsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractРадовановић, Aнита; Милошевић, Иван; Лужајић, Тијана; Милановић, Зорана; Даниловић-Луковић, Јелена; Ковачевић-Филиповић, Милица; Корац, Aлександра;
dc.citation.volume67
dc.citation.issue1
dc.citation.spage92
dc.citation.epage106
dc.citation.other67(1): 92-106
dc.citation.rankM23
dc.identifier.wos000401672900009
dc.identifier.doi10.1515/acve-2017-0009
dc.identifier.scopus2-s2.0-85016747573
dc.identifier.fulltexthttp://veterinar.vet.bg.ac.rs/bitstream/id/437/1476.pdf
dc.identifier.rcubconv_2257
dc.type.versionpublishedVersion


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