Progesterone concentration, pregnancy and calving rate in simmental dairy cows after oestrus synchronisation and HCG treatment during the early luteal phase
2017
Authors
Suluburić, AdamMilanović, Svetlana
Vranješ-Đurić, Sanja
Jovanović, Ivan B.
Barna, Tomislav
Stojić, Milica
Fratrić, Natalija
Szenci, Otto
Gvozdić, Dragan
Article (Published version)
Metadata
Show full item recordAbstract
Early embryonic development may be negatively affected by insufficient progesterone (P4) production. Therefore, the aim of our study was to increase P4 by gonadotropin-releasing hormone (GnRH) and/or human chorionic gonadotropin (hCG) treatments after inducing oestrus by prostaglandin (PG) treatment. Lactating Simmental dairy cows (n = 110), between 1 to 5 lactations, with an average milk production of 6,500 l/305 days, at 40-80 days postpartum were used and grouped as follows: (1) PG + GnRH treatment at AI (GnRH group), (2) PG + hCG treatment at day 7 after AI (hCG group), (3) PG + GnRH at AI + hCG treatment at day 7 after AI (GnRH/hCG group), and (4) spontaneous oestrus (C: control group). All animals were double inseminated (at the time of oestrus detection and 12 +/- 2 h thereafter). Blood serum and milk samples were collected at the day of observed oestrus (day 0), and 14, 21 and 28 days after AI. Serum P4 was determined using a commercial radioimmunoassay (RIA) test (INEP, Zemun)..., and milk P4 was determined using enzyme-linked immunoassay (ELISA) test (NIV Novi Sad). Pregnancy status was confirmed by ultrasonography between days 28 and 35 after AI. Differences of serum or milk P4 medians, pregnancy (and calving) rate were determined using Dunns Multiple Comparison Tests and Z test, respectively. Serum P4 medians were significantly higher at days 14, 21 and 28 after AI in the hCG-treated animals, indicating increased luteal activity, with a similar tendency in whole milk P4 values. Treatment with hCG during the early luteal phase significantly contributed to the maintenance of gestation at days 28-35 after AI, and also increased the calving rate in Simmental dairy cows.
Keywords:
Progesterone / oestrus synchronisation / pregnancy rate / calving rate / Simmental dairy cowsSource:
Acta Veterinaria Hungarica, 2017, 65, 3, 446-458Publisher:
- Akademiai Kiado Rt, Budapest
Funding / projects:
- Biotechnology in the regulation of productive and reproductive status and health in dairy cows (RS-MESTD-Technological Development (TD or TR)-31050)
- Improvement and development of hygienic and technological procedures in production of animal originating foodstuffs with the aim of producing high-quality and safe products competetive on the global market (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46009)
- Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46002)
DOI: 10.1556/004.2017.042
ISSN: 0236-6290
PubMed: 28956489
WoS: 000411907900012
Scopus: 2-s2.0-85030564648
Collections
Institution/Community
Fakultet veterinarske medicineTY - JOUR AU - Suluburić, Adam AU - Milanović, Svetlana AU - Vranješ-Đurić, Sanja AU - Jovanović, Ivan B. AU - Barna, Tomislav AU - Stojić, Milica AU - Fratrić, Natalija AU - Szenci, Otto AU - Gvozdić, Dragan PY - 2017 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1486 AB - Early embryonic development may be negatively affected by insufficient progesterone (P4) production. Therefore, the aim of our study was to increase P4 by gonadotropin-releasing hormone (GnRH) and/or human chorionic gonadotropin (hCG) treatments after inducing oestrus by prostaglandin (PG) treatment. Lactating Simmental dairy cows (n = 110), between 1 to 5 lactations, with an average milk production of 6,500 l/305 days, at 40-80 days postpartum were used and grouped as follows: (1) PG + GnRH treatment at AI (GnRH group), (2) PG + hCG treatment at day 7 after AI (hCG group), (3) PG + GnRH at AI + hCG treatment at day 7 after AI (GnRH/hCG group), and (4) spontaneous oestrus (C: control group). All animals were double inseminated (at the time of oestrus detection and 12 +/- 2 h thereafter). Blood serum and milk samples were collected at the day of observed oestrus (day 0), and 14, 21 and 28 days after AI. Serum P4 was determined using a commercial radioimmunoassay (RIA) test (INEP, Zemun), and milk P4 was determined using enzyme-linked immunoassay (ELISA) test (NIV Novi Sad). Pregnancy status was confirmed by ultrasonography between days 28 and 35 after AI. Differences of serum or milk P4 medians, pregnancy (and calving) rate were determined using Dunns Multiple Comparison Tests and Z test, respectively. Serum P4 medians were significantly higher at days 14, 21 and 28 after AI in the hCG-treated animals, indicating increased luteal activity, with a similar tendency in whole milk P4 values. Treatment with hCG during the early luteal phase significantly contributed to the maintenance of gestation at days 28-35 after AI, and also increased the calving rate in Simmental dairy cows. PB - Akademiai Kiado Rt, Budapest T2 - Acta Veterinaria Hungarica T1 - Progesterone concentration, pregnancy and calving rate in simmental dairy cows after oestrus synchronisation and HCG treatment during the early luteal phase VL - 65 IS - 3 SP - 446 EP - 458 DO - 10.1556/004.2017.042 ER -
@article{ author = "Suluburić, Adam and Milanović, Svetlana and Vranješ-Đurić, Sanja and Jovanović, Ivan B. and Barna, Tomislav and Stojić, Milica and Fratrić, Natalija and Szenci, Otto and Gvozdić, Dragan", year = "2017", abstract = "Early embryonic development may be negatively affected by insufficient progesterone (P4) production. Therefore, the aim of our study was to increase P4 by gonadotropin-releasing hormone (GnRH) and/or human chorionic gonadotropin (hCG) treatments after inducing oestrus by prostaglandin (PG) treatment. Lactating Simmental dairy cows (n = 110), between 1 to 5 lactations, with an average milk production of 6,500 l/305 days, at 40-80 days postpartum were used and grouped as follows: (1) PG + GnRH treatment at AI (GnRH group), (2) PG + hCG treatment at day 7 after AI (hCG group), (3) PG + GnRH at AI + hCG treatment at day 7 after AI (GnRH/hCG group), and (4) spontaneous oestrus (C: control group). All animals were double inseminated (at the time of oestrus detection and 12 +/- 2 h thereafter). Blood serum and milk samples were collected at the day of observed oestrus (day 0), and 14, 21 and 28 days after AI. Serum P4 was determined using a commercial radioimmunoassay (RIA) test (INEP, Zemun), and milk P4 was determined using enzyme-linked immunoassay (ELISA) test (NIV Novi Sad). Pregnancy status was confirmed by ultrasonography between days 28 and 35 after AI. Differences of serum or milk P4 medians, pregnancy (and calving) rate were determined using Dunns Multiple Comparison Tests and Z test, respectively. Serum P4 medians were significantly higher at days 14, 21 and 28 after AI in the hCG-treated animals, indicating increased luteal activity, with a similar tendency in whole milk P4 values. Treatment with hCG during the early luteal phase significantly contributed to the maintenance of gestation at days 28-35 after AI, and also increased the calving rate in Simmental dairy cows.", publisher = "Akademiai Kiado Rt, Budapest", journal = "Acta Veterinaria Hungarica", title = "Progesterone concentration, pregnancy and calving rate in simmental dairy cows after oestrus synchronisation and HCG treatment during the early luteal phase", volume = "65", number = "3", pages = "446-458", doi = "10.1556/004.2017.042" }
Suluburić, A., Milanović, S., Vranješ-Đurić, S., Jovanović, I. B., Barna, T., Stojić, M., Fratrić, N., Szenci, O.,& Gvozdić, D.. (2017). Progesterone concentration, pregnancy and calving rate in simmental dairy cows after oestrus synchronisation and HCG treatment during the early luteal phase. in Acta Veterinaria Hungarica Akademiai Kiado Rt, Budapest., 65(3), 446-458. https://doi.org/10.1556/004.2017.042
Suluburić A, Milanović S, Vranješ-Đurić S, Jovanović IB, Barna T, Stojić M, Fratrić N, Szenci O, Gvozdić D. Progesterone concentration, pregnancy and calving rate in simmental dairy cows after oestrus synchronisation and HCG treatment during the early luteal phase. in Acta Veterinaria Hungarica. 2017;65(3):446-458. doi:10.1556/004.2017.042 .
Suluburić, Adam, Milanović, Svetlana, Vranješ-Đurić, Sanja, Jovanović, Ivan B., Barna, Tomislav, Stojić, Milica, Fratrić, Natalija, Szenci, Otto, Gvozdić, Dragan, "Progesterone concentration, pregnancy and calving rate in simmental dairy cows after oestrus synchronisation and HCG treatment during the early luteal phase" in Acta Veterinaria Hungarica, 65, no. 3 (2017):446-458, https://doi.org/10.1556/004.2017.042 . .