Combined effects of electromagnetic field and low-level laser increase proliferation and alter the morphology of human adipose tissue-derived mesenchymal stem cells
Само за регистроване кориснике
2017
Аутори
Nurković, JasminZaletel, Ivan
Nurković, Selmina
Hajrović, Sefcet
Mustafić, Fahrudin
Isma, Jovan
Jurišić-Skevin, Aleksandra
Grbović, Vesna
Kovačević-Filipović, Milica
Dolićanin, Zana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In recent years, electromagnetic field (EMF) and low-level laser (LLL) have been found to affect various biological processes, the growth and proliferation of cells, and especially that of stem cells. The aim of this study was to investigate the effects of EMF and LLL on proliferation of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) and thus to examine the impact of these therapeutic physical modalities on stem cell engraftment. hAT-MSCs were isolated from subcutaneous adipose tissue of six persons ranging in age from 21 to 56 years. EMF was applied for a period of 7 days, once a day for 30 min, via a magnetic cushion surface at a frequency of 50 Hz and an intensity of 3 mT. LLL was applied also for 7 days, once a day for 5 min, at radiation energies of 3 J/cm(2), with a wavelength of 808 nm, power output of 200 mW, and power density of 0.2 W/cm(2). Nonexposed cells (control) were cultivated under the same culture conditions. Seven days after treatment, the cells were ...examined for cell viability, proliferation, and morphology. We found that after 7 days, the number of EMF-treated hAT-MSCs was significantly higher than the number of the untreated cells, LLL-treated hAT-MSCs were more numerous than EMF-treated cells, and hAT-MSCs that were treated with the combination of EMF and LLL were the most numerous. EMF and/or LLL treatment did not significantly affect hAT-MSC viability by itself. Changes in cell morphology were also observed, in terms of an increase in cell surface area and fractal dimension in hAT-MSCs treated with EMF and the combination of EMF and LLL. In conclusion, EMF and/or LLL treatment accelerated the proliferation of hAT-MSCs without compromising their viability, and therefore, they may be used in stem cell tissue engineering.
Кључне речи:
Mesenchymal stem cells / Electromagnetic field / Low-level laser / Treatment / Therapeutic application / Fractal analysisИзвор:
Lasers in Medical Science, 2017, 32, 1, 151-160Издавач:
- Springer London Ltd, London
Финансирање / пројекти:
- Антиоксидативна заштита и потенцијали за диференцијацију и регенерацију мезенхималних матичних ћелија из различитих ткива током процеса старења (RS-MESTD-Basic Research (BR or ON)-175061)
DOI: 10.1007/s10103-016-2097-2
ISSN: 0268-8921
PubMed: 27770222
WoS: 000392081900019
Scopus: 2-s2.0-84992029084
Колекције
Институција/група
Fakultet veterinarske medicineTY - JOUR AU - Nurković, Jasmin AU - Zaletel, Ivan AU - Nurković, Selmina AU - Hajrović, Sefcet AU - Mustafić, Fahrudin AU - Isma, Jovan AU - Jurišić-Skevin, Aleksandra AU - Grbović, Vesna AU - Kovačević-Filipović, Milica AU - Dolićanin, Zana PY - 2017 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1495 AB - In recent years, electromagnetic field (EMF) and low-level laser (LLL) have been found to affect various biological processes, the growth and proliferation of cells, and especially that of stem cells. The aim of this study was to investigate the effects of EMF and LLL on proliferation of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) and thus to examine the impact of these therapeutic physical modalities on stem cell engraftment. hAT-MSCs were isolated from subcutaneous adipose tissue of six persons ranging in age from 21 to 56 years. EMF was applied for a period of 7 days, once a day for 30 min, via a magnetic cushion surface at a frequency of 50 Hz and an intensity of 3 mT. LLL was applied also for 7 days, once a day for 5 min, at radiation energies of 3 J/cm(2), with a wavelength of 808 nm, power output of 200 mW, and power density of 0.2 W/cm(2). Nonexposed cells (control) were cultivated under the same culture conditions. Seven days after treatment, the cells were examined for cell viability, proliferation, and morphology. We found that after 7 days, the number of EMF-treated hAT-MSCs was significantly higher than the number of the untreated cells, LLL-treated hAT-MSCs were more numerous than EMF-treated cells, and hAT-MSCs that were treated with the combination of EMF and LLL were the most numerous. EMF and/or LLL treatment did not significantly affect hAT-MSC viability by itself. Changes in cell morphology were also observed, in terms of an increase in cell surface area and fractal dimension in hAT-MSCs treated with EMF and the combination of EMF and LLL. In conclusion, EMF and/or LLL treatment accelerated the proliferation of hAT-MSCs without compromising their viability, and therefore, they may be used in stem cell tissue engineering. PB - Springer London Ltd, London T2 - Lasers in Medical Science T1 - Combined effects of electromagnetic field and low-level laser increase proliferation and alter the morphology of human adipose tissue-derived mesenchymal stem cells VL - 32 IS - 1 SP - 151 EP - 160 DO - 10.1007/s10103-016-2097-2 ER -
@article{ author = "Nurković, Jasmin and Zaletel, Ivan and Nurković, Selmina and Hajrović, Sefcet and Mustafić, Fahrudin and Isma, Jovan and Jurišić-Skevin, Aleksandra and Grbović, Vesna and Kovačević-Filipović, Milica and Dolićanin, Zana", year = "2017", abstract = "In recent years, electromagnetic field (EMF) and low-level laser (LLL) have been found to affect various biological processes, the growth and proliferation of cells, and especially that of stem cells. The aim of this study was to investigate the effects of EMF and LLL on proliferation of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) and thus to examine the impact of these therapeutic physical modalities on stem cell engraftment. hAT-MSCs were isolated from subcutaneous adipose tissue of six persons ranging in age from 21 to 56 years. EMF was applied for a period of 7 days, once a day for 30 min, via a magnetic cushion surface at a frequency of 50 Hz and an intensity of 3 mT. LLL was applied also for 7 days, once a day for 5 min, at radiation energies of 3 J/cm(2), with a wavelength of 808 nm, power output of 200 mW, and power density of 0.2 W/cm(2). Nonexposed cells (control) were cultivated under the same culture conditions. Seven days after treatment, the cells were examined for cell viability, proliferation, and morphology. We found that after 7 days, the number of EMF-treated hAT-MSCs was significantly higher than the number of the untreated cells, LLL-treated hAT-MSCs were more numerous than EMF-treated cells, and hAT-MSCs that were treated with the combination of EMF and LLL were the most numerous. EMF and/or LLL treatment did not significantly affect hAT-MSC viability by itself. Changes in cell morphology were also observed, in terms of an increase in cell surface area and fractal dimension in hAT-MSCs treated with EMF and the combination of EMF and LLL. In conclusion, EMF and/or LLL treatment accelerated the proliferation of hAT-MSCs without compromising their viability, and therefore, they may be used in stem cell tissue engineering.", publisher = "Springer London Ltd, London", journal = "Lasers in Medical Science", title = "Combined effects of electromagnetic field and low-level laser increase proliferation and alter the morphology of human adipose tissue-derived mesenchymal stem cells", volume = "32", number = "1", pages = "151-160", doi = "10.1007/s10103-016-2097-2" }
Nurković, J., Zaletel, I., Nurković, S., Hajrović, S., Mustafić, F., Isma, J., Jurišić-Skevin, A., Grbović, V., Kovačević-Filipović, M.,& Dolićanin, Z.. (2017). Combined effects of electromagnetic field and low-level laser increase proliferation and alter the morphology of human adipose tissue-derived mesenchymal stem cells. in Lasers in Medical Science Springer London Ltd, London., 32(1), 151-160. https://doi.org/10.1007/s10103-016-2097-2
Nurković J, Zaletel I, Nurković S, Hajrović S, Mustafić F, Isma J, Jurišić-Skevin A, Grbović V, Kovačević-Filipović M, Dolićanin Z. Combined effects of electromagnetic field and low-level laser increase proliferation and alter the morphology of human adipose tissue-derived mesenchymal stem cells. in Lasers in Medical Science. 2017;32(1):151-160. doi:10.1007/s10103-016-2097-2 .
Nurković, Jasmin, Zaletel, Ivan, Nurković, Selmina, Hajrović, Sefcet, Mustafić, Fahrudin, Isma, Jovan, Jurišić-Skevin, Aleksandra, Grbović, Vesna, Kovačević-Filipović, Milica, Dolićanin, Zana, "Combined effects of electromagnetic field and low-level laser increase proliferation and alter the morphology of human adipose tissue-derived mesenchymal stem cells" in Lasers in Medical Science, 32, no. 1 (2017):151-160, https://doi.org/10.1007/s10103-016-2097-2 . .