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dc.creatorNurković, Jasmin
dc.creatorZaletel, Ivan
dc.creatorNurković, Selmina
dc.creatorHajrović, Sefcet
dc.creatorMustafić, Fahrudin
dc.creatorIsma, Jovan
dc.creatorJurišić-Skevin, Aleksandra
dc.creatorGrbović, Vesna
dc.creatorKovačević-Filipović, Milica
dc.creatorDolićanin, Zana
dc.date.accessioned2020-06-03T14:16:10Z
dc.date.available2020-06-03T14:16:10Z
dc.date.issued2017
dc.identifier.issn0268-8921
dc.identifier.urihttp://vet-erinar.vet.bg.ac.rs/handle/123456789/1495
dc.description.abstractIn recent years, electromagnetic field (EMF) and low-level laser (LLL) have been found to affect various biological processes, the growth and proliferation of cells, and especially that of stem cells. The aim of this study was to investigate the effects of EMF and LLL on proliferation of human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) and thus to examine the impact of these therapeutic physical modalities on stem cell engraftment. hAT-MSCs were isolated from subcutaneous adipose tissue of six persons ranging in age from 21 to 56 years. EMF was applied for a period of 7 days, once a day for 30 min, via a magnetic cushion surface at a frequency of 50 Hz and an intensity of 3 mT. LLL was applied also for 7 days, once a day for 5 min, at radiation energies of 3 J/cm(2), with a wavelength of 808 nm, power output of 200 mW, and power density of 0.2 W/cm(2). Nonexposed cells (control) were cultivated under the same culture conditions. Seven days after treatment, the cells were examined for cell viability, proliferation, and morphology. We found that after 7 days, the number of EMF-treated hAT-MSCs was significantly higher than the number of the untreated cells, LLL-treated hAT-MSCs were more numerous than EMF-treated cells, and hAT-MSCs that were treated with the combination of EMF and LLL were the most numerous. EMF and/or LLL treatment did not significantly affect hAT-MSC viability by itself. Changes in cell morphology were also observed, in terms of an increase in cell surface area and fractal dimension in hAT-MSCs treated with EMF and the combination of EMF and LLL. In conclusion, EMF and/or LLL treatment accelerated the proliferation of hAT-MSCs without compromising their viability, and therefore, they may be used in stem cell tissue engineering.en
dc.publisherSpringer London Ltd, London
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175061/RS//
dc.rightsrestrictedAccess
dc.sourceLasers in Medical Science
dc.subjectMesenchymal stem cellsen
dc.subjectElectromagnetic fielden
dc.subjectLow-level laseren
dc.subjectTreatmenten
dc.subjectTherapeutic applicationen
dc.subjectFractal analysisen
dc.titleCombined effects of electromagnetic field and low-level laser increase proliferation and alter the morphology of human adipose tissue-derived mesenchymal stem cellsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractДолићанин, Зана; Нурковић, Јасмин; Ковачевић-Филиповић, Милица; Залетел, Иван; Грбовић, Весна; Јуришић-Скевин, Aлександра; Исма, Јован; Хајровић, Сефцет; Нурковић, Селмина; Мустафић, Фахрудин;
dc.citation.volume32
dc.citation.issue1
dc.citation.spage151
dc.citation.epage160
dc.citation.other32(1): 151-160
dc.citation.rankM22
dc.identifier.wos000392081900019
dc.identifier.doi10.1007/s10103-016-2097-2
dc.identifier.pmid27770222
dc.identifier.scopus2-s2.0-84992029084
dc.identifier.rcubconv_2230
dc.type.versionpublishedVersion


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