Calcium-dependent antibacterial activity of donkey s milk against Salmonella
Samo za registrovane korisnike
2017
Autori
Sarić, LjubišaPezo, Lato
Sarić, Bojana
Plavsić, Dragana
Jovanov, Pavle
Karabasil, Nedjeljko
Gubić, Jasmina
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The aim of this study was to examine the antibacterial activity of raw donkeys milk (DM) against Salmonella Enteritidis and Salmonella Typhimurium as well as to determine the dependence of this antibacterial activity on calcium, lysozyme and lactoferrin content. Antibacterial assays were conducted in DM artificially contaminated with these Salmonella serotypes and then incubated at 38 degrees C for 8 hours. Calcium concentration was found to have a strong influence on the antibacterial activity of the contaminated DM samples supplemented with CaCl2 and EDTA. The antibacterial activity of DM against the tested Salmonella strains was observed to be strongly calcium dependent, with the addition of CaCl2 to DM samples improving its antibacterial potential against both pathogens. Salmonella Enteritidis appeared to be less sensitive to the antimicrobial agents in DM than S. Typhimurium. One explanation is the calcium-dependant antibacterial activity of DM is attributable to the calcium-bindi...ng ability of its lysozyme. Lysozyme may be the main antibacterial agent, most probably via a nonenzymatic mode of action against tested Salmonella strains.
Ključne reči:
Donkey'smilk / Antibacterial activity / Salmonella / CalciumIzvor:
Annals of Microbiology, 2017, 67, 2, 185-194Izdavač:
- Springer, New York
Finansiranje / projekti:
- Funkcionalni proizvodi na bazi žita namenjeni osobama sa metaboličkim poremećajima (RS-MESTD-Technological Development (TD or TR)-31029)
DOI: 10.1007/s13213-016-1250-2
ISSN: 1590-4261
WoS: 000394088500005
Scopus: 2-s2.0-85007417003
Kolekcije
Institucija/grupa
Fakultet veterinarske medicineTY - JOUR AU - Sarić, Ljubiša AU - Pezo, Lato AU - Sarić, Bojana AU - Plavsić, Dragana AU - Jovanov, Pavle AU - Karabasil, Nedjeljko AU - Gubić, Jasmina PY - 2017 UR - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1499 AB - The aim of this study was to examine the antibacterial activity of raw donkeys milk (DM) against Salmonella Enteritidis and Salmonella Typhimurium as well as to determine the dependence of this antibacterial activity on calcium, lysozyme and lactoferrin content. Antibacterial assays were conducted in DM artificially contaminated with these Salmonella serotypes and then incubated at 38 degrees C for 8 hours. Calcium concentration was found to have a strong influence on the antibacterial activity of the contaminated DM samples supplemented with CaCl2 and EDTA. The antibacterial activity of DM against the tested Salmonella strains was observed to be strongly calcium dependent, with the addition of CaCl2 to DM samples improving its antibacterial potential against both pathogens. Salmonella Enteritidis appeared to be less sensitive to the antimicrobial agents in DM than S. Typhimurium. One explanation is the calcium-dependant antibacterial activity of DM is attributable to the calcium-binding ability of its lysozyme. Lysozyme may be the main antibacterial agent, most probably via a nonenzymatic mode of action against tested Salmonella strains. PB - Springer, New York T2 - Annals of Microbiology T1 - Calcium-dependent antibacterial activity of donkey s milk against Salmonella VL - 67 IS - 2 SP - 185 EP - 194 DO - 10.1007/s13213-016-1250-2 ER -
@article{ author = "Sarić, Ljubiša and Pezo, Lato and Sarić, Bojana and Plavsić, Dragana and Jovanov, Pavle and Karabasil, Nedjeljko and Gubić, Jasmina", year = "2017", abstract = "The aim of this study was to examine the antibacterial activity of raw donkeys milk (DM) against Salmonella Enteritidis and Salmonella Typhimurium as well as to determine the dependence of this antibacterial activity on calcium, lysozyme and lactoferrin content. Antibacterial assays were conducted in DM artificially contaminated with these Salmonella serotypes and then incubated at 38 degrees C for 8 hours. Calcium concentration was found to have a strong influence on the antibacterial activity of the contaminated DM samples supplemented with CaCl2 and EDTA. The antibacterial activity of DM against the tested Salmonella strains was observed to be strongly calcium dependent, with the addition of CaCl2 to DM samples improving its antibacterial potential against both pathogens. Salmonella Enteritidis appeared to be less sensitive to the antimicrobial agents in DM than S. Typhimurium. One explanation is the calcium-dependant antibacterial activity of DM is attributable to the calcium-binding ability of its lysozyme. Lysozyme may be the main antibacterial agent, most probably via a nonenzymatic mode of action against tested Salmonella strains.", publisher = "Springer, New York", journal = "Annals of Microbiology", title = "Calcium-dependent antibacterial activity of donkey s milk against Salmonella", volume = "67", number = "2", pages = "185-194", doi = "10.1007/s13213-016-1250-2" }
Sarić, L., Pezo, L., Sarić, B., Plavsić, D., Jovanov, P., Karabasil, N.,& Gubić, J.. (2017). Calcium-dependent antibacterial activity of donkey s milk against Salmonella. in Annals of Microbiology Springer, New York., 67(2), 185-194. https://doi.org/10.1007/s13213-016-1250-2
Sarić L, Pezo L, Sarić B, Plavsić D, Jovanov P, Karabasil N, Gubić J. Calcium-dependent antibacterial activity of donkey s milk against Salmonella. in Annals of Microbiology. 2017;67(2):185-194. doi:10.1007/s13213-016-1250-2 .
Sarić, Ljubiša, Pezo, Lato, Sarić, Bojana, Plavsić, Dragana, Jovanov, Pavle, Karabasil, Nedjeljko, Gubić, Jasmina, "Calcium-dependent antibacterial activity of donkey s milk against Salmonella" in Annals of Microbiology, 67, no. 2 (2017):185-194, https://doi.org/10.1007/s13213-016-1250-2 . .