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dc.creatorStojić, Milica
dc.creatorFratrić, Natalija
dc.creatorKovacić, Marijana
dc.creatorIlić, Vesna
dc.creatorGvozdić, Dragan
dc.creatorSavić, Olivera
dc.creatorDoković, Radojica
dc.creatorValčić, Olivera
dc.date.accessioned2020-06-03T14:17:52Z
dc.date.available2020-06-03T14:17:52Z
dc.date.issued2017
dc.identifier.issn0567-8315
dc.identifier.urihttps://vet-erinar.vet.bg.ac.rs/handle/123456789/1519
dc.description.abstractFailure of passive transfer (FPT) of immunoglobulins (IgG) is associated with increased morbidity and mortality of calves. In this study we evaluated the digital Brix refractometer usefulness for the assessment of FPT. A number of 16 colostrum samples from the first milking (2-3h post-partum) of Holstein-Friesian dairy heifers and 29 blood sera of 3-6 days old calves were analyzed with a digital Brix refractometer. Total proteins were determined by the biuret reaction. Colostral IgG were determined by radial immunodiffusion (RID), and colostral whey and blood serum. globulin (composed almost entirely of IgG) were determined by agarose protein gel electrophoresis (APE) and densitometry. Colostral % Brix score was 25.5 +/- 3.4%. Concentrations of colostrum IgG and colostral whey. globulin were 130 +/- 33 g/L and 100 +/- 24 g/L respectively. The concentration of total proteins in colostral whey was 134 +/- 30 g/L. The correlations between Brix values and the concentrations of IgG determined with RID and the concentrations of. globulin determined with APE were positive and highly significant (P<0.001 and P<0.01). The concentration of serum proteins of new-born calves was 57.75 +/- 11.8 g/L, the concentration of. globulin was 14.4 +/- 7.8 g/L, and the Brix score was 8.6 +/- 1.0%. FPT (serum. globulin<10 g/L) was detected in 34.5% (10/29) calves. Brix score correlated with the concentration of blood serum. globulins in all examined calves. The results have confirmed that digital Brix refractometry allows the producers to use this technique in order to estimate colostral and calf serum IgG, thereby monitoring both colostrum quality and success of passive transfer.en
dc.publisherUniverzitet u Beogradu - Fakultet veterinarske medicine, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46002/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31050/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS//
dc.rightsopenAccess
dc.sourceActa Veterinaria-Beograd
dc.subjectagarose gel protein electrophoresisen
dc.subjectbrix refractometeren
dc.subjectcolostrum IgGen
dc.subjectfailure of passive transferen
dc.subjectnew-born calf sera IgGen
dc.subjectradial immunodiffusionen
dc.titleBrix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transferen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractФратрић, Наталија; Илић, Весна; Стојић, Милица; Ковацић, Маријана; Гвоздић, Драган; Савић, Оливера; Валчић, Оливера; Доковић, Радојица;
dc.citation.volume67
dc.citation.issue4
dc.citation.spage508
dc.citation.epage524
dc.citation.other67(4): 508-524
dc.citation.rankM23
dc.identifier.wos000419653200005
dc.identifier.doi10.1515/acve-2017-0041
dc.identifier.scopus2-s2.0-85041206212
dc.identifier.fulltexthttps://vet-erinar.vet.bg.ac.rs/bitstream/id/480/1518.pdf
dc.type.versionpublishedVersion


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